ID   RNY_ACHLI               Reviewed;         526 AA.
AC   A9NGL9;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-FEB-2008, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Ribonuclease Y {ECO:0000255|HAMAP-Rule:MF_00335};
DE            Short=RNase Y {ECO:0000255|HAMAP-Rule:MF_00335};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00335};
GN   Name=rny {ECO:0000255|HAMAP-Rule:MF_00335}; OrderedLocusNames=ACL_0886;
OS   Acholeplasma laidlawii (strain PG-8A).
OC   Bacteria; Tenericutes; Mollicutes; Acholeplasmatales; Acholeplasmataceae;
OC   Acholeplasma.
OX   NCBI_TaxID=441768;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PG-8A;
RX   PubMed=21784942; DOI=10.1128/jb.05059-11;
RA   Lazarev V.N., Levitskii S.A., Basovskii Y.I., Chukin M.M., Akopian T.A.,
RA   Vereshchagin V.V., Kostrjukova E.S., Kovaleva G.Y., Kazanov M.D.,
RA   Malko D.B., Vitreschak A.G., Sernova N.V., Gelfand M.S., Demina I.A.,
RA   Serebryakova M.V., Galyamina M.A., Vtyurin N.N., Rogov S.I., Alexeev D.G.,
RA   Ladygina V.G., Govorun V.M.;
RT   "Complete genome and proteome of Acholeplasma laidlawii.";
RL   J. Bacteriol. 193:4943-4953(2011).
CC   -!- FUNCTION: Endoribonuclease that initiates mRNA decay.
CC       {ECO:0000255|HAMAP-Rule:MF_00335}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_00335};
CC       Single-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_00335}.
CC   -!- SIMILARITY: Belongs to the RNase Y family. {ECO:0000255|HAMAP-
CC       Rule:MF_00335}.
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DR   EMBL; CP000896; ABX81499.1; -; Genomic_DNA.
DR   RefSeq; WP_012242830.1; NC_010163.1.
DR   AlphaFoldDB; A9NGL9; -.
DR   SMR; A9NGL9; -.
DR   STRING; 441768.ACL_0886; -.
DR   PRIDE; A9NGL9; -.
DR   EnsemblBacteria; ABX81499; ABX81499; ACL_0886.
DR   GeneID; 66293872; -.
DR   KEGG; acl:ACL_0886; -.
DR   eggNOG; COG1418; Bacteria.
DR   eggNOG; COG4372; Bacteria.
DR   HOGENOM; CLU_028328_1_0_14; -.
DR   OMA; PHAILGM; -.
DR   OrthoDB; 1012190at2; -.
DR   Proteomes; UP000008558; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004521; F:endoribonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006402; P:mRNA catabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd00077; HDc; 1.
DR   Gene3D; 3.30.1370.10; -; 1.
DR   HAMAP; MF_00335; RNase_Y; 1.
DR   InterPro; IPR003607; HD/PDEase_dom.
DR   InterPro; IPR006674; HD_domain.
DR   InterPro; IPR006675; HDIG_dom.
DR   InterPro; IPR004087; KH_dom.
DR   InterPro; IPR004088; KH_dom_type_1.
DR   InterPro; IPR036612; KH_dom_type_1_sf.
DR   InterPro; IPR017705; Ribonuclease_Y.
DR   InterPro; IPR022711; RNase_Y_N.
DR   PANTHER; PTHR12826:SF15; PTHR12826:SF15; 1.
DR   Pfam; PF01966; HD; 1.
DR   Pfam; PF00013; KH_1; 1.
DR   Pfam; PF12072; RNase_Y_N; 1.
DR   SMART; SM00471; HDc; 1.
DR   SMART; SM00322; KH; 1.
DR   SUPFAM; SSF54791; SSF54791; 1.
DR   TIGRFAMs; TIGR00277; HDIG; 1.
DR   TIGRFAMs; TIGR03319; RNase_Y; 1.
DR   PROSITE; PS51831; HD; 1.
DR   PROSITE; PS50084; KH_TYPE_1; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Endonuclease; Hydrolase; Membrane; Nuclease;
KW   Reference proteome; RNA-binding; Transmembrane; Transmembrane helix.
FT   CHAIN           1..526
FT                   /note="Ribonuclease Y"
FT                   /id="PRO_0000344806"
FT   TRANSMEM        10..30
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00335"
FT   DOMAIN          216..279
FT                   /note="KH"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00335"
FT   DOMAIN          342..435
FT                   /note="HD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01175"
SQ   SEQUENCE   526 AA;  59045 MW;  C21BC4FEF13162AF CRC64;
     MFNVDTPALI TFILLIVVGA LGGALVGYFI RVAQHEKSLR LAREEAERII EDGKKEADRT
     KREMVFEAKQ EILTLRKEFD EDIKDRRQIV MNLEEKATQR ENALNQRSQY LDKREIGLDA
     KEERHNERKE QLDIQYSKVE ELIKEQEEKL SSISALSREQ ARELIMAQVR DSISNEIAAY
     IRDEEDNAKS IAQNKSKEIL SLAMQKYAAE TTSERTVTVV EIPNEDMKGR IIGKEGRNIR
     SLEALTGVDL IIDDTPEAVV LSGFDPVRRE VAKRALTILV QDGRIHPGRI EEVVERARTE
     IDMFIREAGE EAVFKTGVGK VHPDIIKLLG RMTFRTSYGQ NVLKHSIEVA FLAGKLAAEI
     GENEMLARRA GLFHDIGKAI DHEVEGSHVS IGVELMSRYK EPKEVIDAIA SHHGDSEPES
     IIAVLVAAAD ALSAARPGAR SESMDSYMKR LTQLEEISND VTGVDKAYAI QAGREVRVMV
     LPDKVDDLGL INIARTIKEK IEAQMTYPGT IKVTVIREKR ATDVAK