ID   RNY_AQUAE               Reviewed;         558 AA.
AC   O67622;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 132.
DE   RecName: Full=Ribonuclease Y {ECO:0000255|HAMAP-Rule:MF_00335};
DE            Short=RNase Y {ECO:0000255|HAMAP-Rule:MF_00335};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00335};
GN   Name=rny {ECO:0000255|HAMAP-Rule:MF_00335}; OrderedLocusNames=aq_1732;
OS   Aquifex aeolicus (strain VF5).
OC   Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex.
OX   NCBI_TaxID=224324;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=VF5;
RX   PubMed=9537320; DOI=10.1038/32831;
RA   Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA   Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA   Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT   "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.";
RL   Nature 392:353-358(1998).
CC   -!- FUNCTION: Endoribonuclease that initiates mRNA decay.
CC       {ECO:0000255|HAMAP-Rule:MF_00335}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_00335};
CC       Single-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_00335}.
CC   -!- SIMILARITY: Belongs to the RNase Y family. {ECO:0000255|HAMAP-
CC       Rule:MF_00335}.
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DR   EMBL; AE000657; AAC07588.1; -; Genomic_DNA.
DR   PIR; D70449; D70449.
DR   RefSeq; NP_214188.1; NC_000918.1.
DR   RefSeq; WP_010881125.1; NC_000918.1.
DR   AlphaFoldDB; O67622; -.
DR   SMR; O67622; -.
DR   STRING; 224324.aq_1732; -.
DR   PRIDE; O67622; -.
DR   EnsemblBacteria; AAC07588; AAC07588; aq_1732.
DR   KEGG; aae:aq_1732; -.
DR   PATRIC; fig|224324.8.peg.1335; -.
DR   eggNOG; COG1418; Bacteria.
DR   eggNOG; COG3599; Bacteria.
DR   HOGENOM; CLU_028328_1_0_0; -.
DR   InParanoid; O67622; -.
DR   OMA; PHAILGM; -.
DR   OrthoDB; 1012190at2; -.
DR   Proteomes; UP000000798; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004521; F:endoribonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006402; P:mRNA catabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd00077; HDc; 1.
DR   Gene3D; 3.30.1370.10; -; 1.
DR   HAMAP; MF_00335; RNase_Y; 1.
DR   InterPro; IPR003607; HD/PDEase_dom.
DR   InterPro; IPR006674; HD_domain.
DR   InterPro; IPR006675; HDIG_dom.
DR   InterPro; IPR004087; KH_dom.
DR   InterPro; IPR004088; KH_dom_type_1.
DR   InterPro; IPR036612; KH_dom_type_1_sf.
DR   InterPro; IPR017705; Ribonuclease_Y.
DR   InterPro; IPR022711; RNase_Y_N.
DR   PANTHER; PTHR12826:SF15; PTHR12826:SF15; 1.
DR   Pfam; PF01966; HD; 1.
DR   Pfam; PF00013; KH_1; 1.
DR   Pfam; PF12072; RNase_Y_N; 1.
DR   SMART; SM00471; HDc; 1.
DR   SMART; SM00322; KH; 1.
DR   SUPFAM; SSF54791; SSF54791; 1.
DR   TIGRFAMs; TIGR00277; HDIG; 1.
DR   TIGRFAMs; TIGR03319; RNase_Y; 1.
DR   PROSITE; PS51831; HD; 1.
DR   PROSITE; PS50084; KH_TYPE_1; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Endonuclease; Hydrolase; Membrane; Nuclease;
KW   Reference proteome; RNA-binding; Transmembrane; Transmembrane helix.
FT   CHAIN           1..558
FT                   /note="Ribonuclease Y"
FT                   /id="PRO_0000163763"
FT   TRANSMEM        3..23
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00335"
FT   DOMAIN          248..311
FT                   /note="KH"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00335"
FT   DOMAIN          374..467
FT                   /note="HD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01175"
SQ   SEQUENCE   558 AA;  64329 MW;  FCA1163ED060C2F1 CRC64;
     MDVLSILLIL VAVGVGIFVG RQFLGQKQAP APTYQPVPSP QILEEAKSKA EEIIKEAKEK
     AEVILKEAKE SAEKIVREAE EKAEKLIREA KEEVERIKEE VERRKKELKE REENVLAKER
     HLDRRWEALE KREEELLHRE RELKDFERSL ERWRDEIRHK EEELKHMKEE VEELKKKELE
     ELQRIAKLTL EEARQEIIKK VEEEAKKDAV KLMKVIEEDA KRRAEFEAKK IIATATQRLA
     PQIAVNYTTT TVELPSNEFK GRIIGREGRN IRTFEILTGV DLIIDDTPDI VTISSFDPLR
     REIAKEALQR LIADGRIHPA RIEEVVDEVK REFDEKIRKI GEETVYELDL HDINPGLYYY
     IGKLYFRTSY SQNVLLHSKE VAYIAGLMAE ELGLDAKLAR RAGLLHDIGK AISHELGGSH
     TDIGVELARK YGEPDAVINA IRAHHEEEPV RYPEVALVCA ADALSAARPG ARRETLEAYI
     RRLEKLEEIV KSFKGVANAY AVQAGREVRV IVNPEEISDE EAYLLSKEIP KKIEEELDFP
     GQIKVVVIRE TRHVEYAK