ID   RNY_BORAP               Reviewed;         509 AA.
AC   Q0SN03; G0ISE7;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=Ribonuclease Y {ECO:0000255|HAMAP-Rule:MF_00335};
DE            Short=RNase Y {ECO:0000255|HAMAP-Rule:MF_00335};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00335};
GN   Name=rny {ECO:0000255|HAMAP-Rule:MF_00335};
GN   OrderedLocusNames=BAPKO_0532, BafPKo_0520;
OS   Borreliella afzelii (strain PKo) (Borrelia afzelii).
OC   Bacteria; Spirochaetes; Spirochaetales; Borreliaceae; Borreliella.
OX   NCBI_TaxID=390236;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PKo;
RX   PubMed=16914037; DOI=10.1186/1471-2164-7-211;
RA   Gloeckner G., Schulte-Spechtel U., Schilhabel M., Felder M., Suehnel J.,
RA   Wilske B., Platzer M.;
RT   "Comparative genome analysis: selection pressure on the Borrelia vls
RT   cassettes is essential for infectivity.";
RL   BMC Genomics 7:211-211(2006).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PKo;
RX   PubMed=22123755; DOI=10.1128/jb.05951-11;
RA   Casjens S.R., Mongodin E.F., Qiu W.G., Dunn J.J., Luft B.J.,
RA   Fraser-Liggett C.M., Schutzer S.E.;
RT   "Whole-genome sequences of two Borrelia afzelii and two Borrelia garinii
RT   Lyme disease agent isolates.";
RL   J. Bacteriol. 193:6995-6996(2011).
CC   -!- FUNCTION: Endoribonuclease that initiates mRNA decay.
CC       {ECO:0000255|HAMAP-Rule:MF_00335}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_00335};
CC       Single-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_00335}.
CC   -!- SIMILARITY: Belongs to the RNase Y family. {ECO:0000255|HAMAP-
CC       Rule:MF_00335}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AEL69728.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; CP000395; ABH01775.1; -; Genomic_DNA.
DR   EMBL; CP002933; AEL69728.1; ALT_INIT; Genomic_DNA.
DR   AlphaFoldDB; Q0SN03; -.
DR   STRING; 390236.BafPKo_0520; -.
DR   EnsemblBacteria; AEL69728; AEL69728; BafPKo_0520.
DR   KEGG; baf:BAPKO_0532; -.
DR   KEGG; bafz:BafPKo_0520; -.
DR   PATRIC; fig|390236.22.peg.501; -.
DR   eggNOG; COG1418; Bacteria.
DR   HOGENOM; CLU_028328_1_0_12; -.
DR   OMA; PHAILGM; -.
DR   Proteomes; UP000005216; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004521; F:endoribonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006402; P:mRNA catabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd00077; HDc; 1.
DR   HAMAP; MF_00335; RNase_Y; 1.
DR   InterPro; IPR003607; HD/PDEase_dom.
DR   InterPro; IPR006674; HD_domain.
DR   InterPro; IPR006675; HDIG_dom.
DR   InterPro; IPR004087; KH_dom.
DR   InterPro; IPR004088; KH_dom_type_1.
DR   InterPro; IPR036612; KH_dom_type_1_sf.
DR   InterPro; IPR017705; Ribonuclease_Y.
DR   InterPro; IPR022711; RNase_Y_N.
DR   PANTHER; PTHR12826:SF15; PTHR12826:SF15; 1.
DR   Pfam; PF01966; HD; 1.
DR   Pfam; PF00013; KH_1; 1.
DR   Pfam; PF12072; RNase_Y_N; 1.
DR   SMART; SM00471; HDc; 1.
DR   SMART; SM00322; KH; 1.
DR   SUPFAM; SSF54791; SSF54791; 1.
DR   TIGRFAMs; TIGR00277; HDIG; 1.
DR   TIGRFAMs; TIGR03319; RNase_Y; 1.
DR   PROSITE; PS51831; HD; 1.
DR   PROSITE; PS50084; KH_TYPE_1; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Endonuclease; Hydrolase; Membrane; Nuclease; RNA-binding;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..509
FT                   /note="Ribonuclease Y"
FT                   /id="PRO_0000344829"
FT   TRANSMEM        3..23
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00335"
FT   DOMAIN          197..257
FT                   /note="KH"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00335"
FT   DOMAIN          323..418
FT                   /note="HD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01175"
SQ   SEQUENCE   509 AA;  57919 MW;  5EF929DC473F45B8 CRC64;
     MYIIFSSIFA GFILGFLIRV FLGRLSLLDL EKNLTKVRVE SQLEIENERR QIIANAKSQM
     LKEKNQQDRD IRDRKNEIVN LEKRLLQREE TLDKRISALD KQQSRVDFKI KEFEQKEKVI
     REKEADLVKR LENISGLTRE DARKIVIEKV EHESRRDAQV IINKSEQEAQ LLADKVAKDI
     LVSTMQRIVT EVSSEFTVAS VELPNDEMKG RIIGKEGRNI RALETLIGAD IIIDDTPEAV
     VISCFDPIRK ELAKRTLERL VTDGRIHPAR IEEVVYNVTN EINSIIQEEG EKVVFDLNIH
     GLDKRLIRGL GRLYFRSSYG QNVLSHSKET AIIGEILAKE MKLDPIVVKR ACLLHDIGKG
     MESISDNSEG HAITGAELAQ SCGESEIVVN AIAAHHNEVK PESLEAIVVQ IADAISASRP
     GARRESLNNY INRLKRLEDI AYSFEGVQKC YAIQAGREVR IIVDNALIND EKSILLARDI
     AKKIEAEMRY PGKIKVTIIR ETRVIEYAR