ID   RNY_CLOPE               Reviewed;         511 AA.
AC   Q8XJT1;
DT   20-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT   22-JUL-2008, sequence version 2.
DT   25-MAY-2022, entry version 106.
DE   RecName: Full=Ribonuclease Y {ECO:0000255|HAMAP-Rule:MF_00335};
DE            Short=RNase Y {ECO:0000255|HAMAP-Rule:MF_00335};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00335};
GN   Name=rny {ECO:0000255|HAMAP-Rule:MF_00335}; OrderedLocusNames=CPE1672;
OS   Clostridium perfringens (strain 13 / Type A).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=195102;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=13 / Type A;
RX   PubMed=11792842; DOI=10.1073/pnas.022493799;
RA   Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T.,
RA   Ogasawara N., Hattori M., Kuhara S., Hayashi H.;
RT   "Complete genome sequence of Clostridium perfringens, an anaerobic flesh-
RT   eater.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002).
CC   -!- FUNCTION: Endoribonuclease that initiates mRNA decay.
CC       {ECO:0000255|HAMAP-Rule:MF_00335}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_00335};
CC       Single-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_00335}.
CC   -!- SIMILARITY: Belongs to the RNase Y family. {ECO:0000255|HAMAP-
CC       Rule:MF_00335}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB81378.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; BA000016; BAB81378.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_003459753.1; NC_003366.1.
DR   AlphaFoldDB; Q8XJT1; -.
DR   STRING; 195102.gene:10490936; -.
DR   EnsemblBacteria; BAB81378; BAB81378; BAB81378.
DR   GeneID; 29570974; -.
DR   KEGG; cpe:CPE1672; -.
DR   HOGENOM; CLU_028328_1_0_9; -.
DR   OMA; PHAILGM; -.
DR   Proteomes; UP000000818; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004521; F:endoribonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006402; P:mRNA catabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd00077; HDc; 1.
DR   Gene3D; 3.30.1370.10; -; 1.
DR   HAMAP; MF_00335; RNase_Y; 1.
DR   InterPro; IPR003607; HD/PDEase_dom.
DR   InterPro; IPR006674; HD_domain.
DR   InterPro; IPR006675; HDIG_dom.
DR   InterPro; IPR004087; KH_dom.
DR   InterPro; IPR004088; KH_dom_type_1.
DR   InterPro; IPR036612; KH_dom_type_1_sf.
DR   InterPro; IPR017705; Ribonuclease_Y.
DR   InterPro; IPR022711; RNase_Y_N.
DR   PANTHER; PTHR12826:SF15; PTHR12826:SF15; 1.
DR   Pfam; PF01966; HD; 1.
DR   Pfam; PF00013; KH_1; 1.
DR   Pfam; PF12072; RNase_Y_N; 1.
DR   SMART; SM00471; HDc; 1.
DR   SMART; SM00322; KH; 1.
DR   SUPFAM; SSF54791; SSF54791; 1.
DR   TIGRFAMs; TIGR00277; HDIG; 1.
DR   TIGRFAMs; TIGR03319; RNase_Y; 1.
DR   PROSITE; PS51831; HD; 1.
DR   PROSITE; PS50084; KH_TYPE_1; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Endonuclease; Hydrolase; Membrane; Nuclease;
KW   Reference proteome; RNA-binding; Transmembrane; Transmembrane helix.
FT   CHAIN           1..511
FT                   /note="Ribonuclease Y"
FT                   /id="PRO_0000163771"
FT   TRANSMEM        3..23
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00335"
FT   DOMAIN          201..286
FT                   /note="KH"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00335"
FT   DOMAIN          327..420
FT                   /note="HD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01175"
SQ   SEQUENCE   511 AA;  57681 MW;  DF65E9021EC53EF6 CRC64;
     MVVGILIGII ILGVVGFIQY TLIQKASKNR VESLEKEASL TLEEAKREAE STKKEAILEA
     KEEVHKLRAD LDKETRDRRN EIQRFERRLI QREESLDKKG EMLEKREDSI NKKSIEIQEL
     EERVQNLYGE QRAELERISN LSSEDARTLL LDEVRREIKH ESAMLIKELE TKAKEEADKK
     SREIITTAIQ RCAADHVSET TVHVVALPND EMKGRIIGRE GRNIRTLETL TGVDLIIDDT
     PEAVILSSFD PIRREVARIA LEKLIVDGRI HPARIEEMVE RAIKDVENDI KEEGEQATFE
     TGVHGLHPEI IKLLGRLKYR TSYGQNVLKH SIEVSYLAGL MASELGLDVN LARRAGLLHD
     IGKGVDQEYE GPHAVIGGEL AKKYHESPAV VNAIAAHHGD TEMQTLEAVL VQAADAISAA
     RPGARRETLE AYIKRLEKLE EIATSYEGVE KSYAIQAGRE IRIMVKPDQV DDAGAIEMAR
     NIVKKIEEQL EYPGQIKINV IRETRAVDYA K