ID   RNY_LACAC               Reviewed;         543 AA.
AC   Q5FL81;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Ribonuclease Y {ECO:0000255|HAMAP-Rule:MF_00335};
DE            Short=RNase Y {ECO:0000255|HAMAP-Rule:MF_00335};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00335};
GN   Name=rny {ECO:0000255|HAMAP-Rule:MF_00335}; OrderedLocusNames=LBA0667;
OS   Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactobacillus.
OX   NCBI_TaxID=272621;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700396 / NCK56 / N2 / NCFM;
RX   PubMed=15671160; DOI=10.1073/pnas.0409188102;
RA   Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L.,
RA   McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S.,
RA   Hamrick A., Cano R., Klaenhammer T.R.;
RT   "Complete genome sequence of the probiotic lactic acid bacterium
RT   Lactobacillus acidophilus NCFM.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005).
CC   -!- FUNCTION: Endoribonuclease that initiates mRNA decay.
CC       {ECO:0000255|HAMAP-Rule:MF_00335}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_00335};
CC       Single-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_00335}.
CC   -!- SIMILARITY: Belongs to the RNase Y family. {ECO:0000255|HAMAP-
CC       Rule:MF_00335}.
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DR   EMBL; CP000033; AAV42543.1; -; Genomic_DNA.
DR   RefSeq; WP_011254219.1; NC_006814.3.
DR   RefSeq; YP_193574.1; NC_006814.3.
DR   AlphaFoldDB; Q5FL81; -.
DR   SMR; Q5FL81; -.
DR   STRING; 272621.LBA0667; -.
DR   PRIDE; Q5FL81; -.
DR   EnsemblBacteria; AAV42543; AAV42543; LBA0667.
DR   GeneID; 56942299; -.
DR   KEGG; lac:LBA0667; -.
DR   PATRIC; fig|272621.13.peg.637; -.
DR   eggNOG; COG1418; Bacteria.
DR   HOGENOM; CLU_028328_1_0_9; -.
DR   OMA; PHAILGM; -.
DR   BioCyc; LACI272621:G1G49-689-MON; -.
DR   Proteomes; UP000006381; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004521; F:endoribonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006402; P:mRNA catabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd00077; HDc; 1.
DR   Gene3D; 3.30.1370.10; -; 1.
DR   HAMAP; MF_00335; RNase_Y; 1.
DR   InterPro; IPR003607; HD/PDEase_dom.
DR   InterPro; IPR006674; HD_domain.
DR   InterPro; IPR006675; HDIG_dom.
DR   InterPro; IPR004087; KH_dom.
DR   InterPro; IPR004088; KH_dom_type_1.
DR   InterPro; IPR036612; KH_dom_type_1_sf.
DR   InterPro; IPR017705; Ribonuclease_Y.
DR   InterPro; IPR022711; RNase_Y_N.
DR   PANTHER; PTHR12826:SF15; PTHR12826:SF15; 1.
DR   Pfam; PF01966; HD; 1.
DR   Pfam; PF00013; KH_1; 1.
DR   Pfam; PF12072; RNase_Y_N; 1.
DR   SMART; SM00471; HDc; 1.
DR   SMART; SM00322; KH; 1.
DR   SUPFAM; SSF54791; SSF54791; 1.
DR   TIGRFAMs; TIGR00277; HDIG; 1.
DR   TIGRFAMs; TIGR03319; RNase_Y; 1.
DR   PROSITE; PS51831; HD; 1.
DR   PROSITE; PS50084; KH_TYPE_1; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Endonuclease; Hydrolase; Membrane; Nuclease;
KW   Reference proteome; RNA-binding; Transmembrane; Transmembrane helix.
FT   CHAIN           1..543
FT                   /note="Ribonuclease Y"
FT                   /id="PRO_0000344888"
FT   TRANSMEM        4..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00335"
FT   DOMAIN          233..296
FT                   /note="KH"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00335"
FT   DOMAIN          359..452
FT                   /note="HD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01175"
SQ   SEQUENCE   543 AA;  60616 MW;  D3F1C3EF20E99E49 CRC64;
     MNTIIMIPVA TAIVSLLVGT VTGYAIRKHS WEQKAQNAQN DADHILADAK AQVAAAEAEV
     NAQKQAAIAV KQSAENTKKE KILEAQEQIR DYRQKTEDEL NSKKDNLARQ ENRLQQREDT
     LDHKNSLLDE REAGLTEKED QLKQQNASLK AKLDEADELV GIRRQKLYDV AKLDKDQAKK
     IVLDQLSDEL VKERAEMIRN SNEEVMAKAD RYANQVIIDA IQSSAADTVA ETTVSVVDLP
     NEEMKGRIIG REGRNIRSFE ALTGIDLIID DTPKVVTLSG FDPIRREIAK RAMERLIKDG
     RIHPARIEEM VDKARKEVND DIYEAGESAL MELGIHRMNP ELVKTLGRLK YHTSYGQNVL
     SHSIEVGKLA GTMAAELGLD EKLAVRAGLL HDIGKAIDHD IEGSHVEIGV ELTRKYHEPD
     VVVNAIAAHH GDVPKLSFIA ELVVAADTIS SARPGARSES LENYIRRLTD LEKIAKSYQG
     VKQAYAIQAG REVRVMVEPD EISDDRTVIL ARDIRNQVEK ELDYPGNIKI TVIREKRVVA
     IAK