ID   RNY_LACLA               Reviewed;         531 AA.
AC   Q9CEE2;
DT   20-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 110.
DE   RecName: Full=Ribonuclease Y {ECO:0000255|HAMAP-Rule:MF_00335};
DE            Short=RNase Y {ECO:0000255|HAMAP-Rule:MF_00335};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00335};
GN   Name=rny {ECO:0000255|HAMAP-Rule:MF_00335}; Synonyms=ytgF;
GN   OrderedLocusNames=LL1900; ORFNames=L150593;
OS   Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=272623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IL1403;
RX   PubMed=11337471; DOI=10.1101/gr.gr-1697r;
RA   Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J.,
RA   Ehrlich S.D., Sorokin A.;
RT   "The complete genome sequence of the lactic acid bacterium Lactococcus
RT   lactis ssp. lactis IL1403.";
RL   Genome Res. 11:731-753(2001).
CC   -!- FUNCTION: Endoribonuclease that initiates mRNA decay.
CC       {ECO:0000255|HAMAP-Rule:MF_00335}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_00335};
CC       Single-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_00335}.
CC   -!- SIMILARITY: Belongs to the RNase Y family. {ECO:0000255|HAMAP-
CC       Rule:MF_00335}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE005176; AAK05998.1; -; Genomic_DNA.
DR   PIR; D86862; D86862.
DR   RefSeq; NP_268057.1; NC_002662.1.
DR   RefSeq; WP_003131467.1; NC_002662.1.
DR   AlphaFoldDB; Q9CEE2; -.
DR   SMR; Q9CEE2; -.
DR   STRING; 272623.L150593; -.
DR   PaxDb; Q9CEE2; -.
DR   EnsemblBacteria; AAK05998; AAK05998; L150593.
DR   GeneID; 60355192; -.
DR   KEGG; lla:L150593; -.
DR   PATRIC; fig|272623.7.peg.2035; -.
DR   eggNOG; COG1418; Bacteria.
DR   HOGENOM; CLU_028328_1_0_9; -.
DR   OMA; PHAILGM; -.
DR   Proteomes; UP000002196; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004521; F:endoribonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006402; P:mRNA catabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd00077; HDc; 1.
DR   Gene3D; 3.30.1370.10; -; 1.
DR   HAMAP; MF_00335; RNase_Y; 1.
DR   InterPro; IPR003607; HD/PDEase_dom.
DR   InterPro; IPR006674; HD_domain.
DR   InterPro; IPR006675; HDIG_dom.
DR   InterPro; IPR004087; KH_dom.
DR   InterPro; IPR004088; KH_dom_type_1.
DR   InterPro; IPR036612; KH_dom_type_1_sf.
DR   InterPro; IPR017705; Ribonuclease_Y.
DR   InterPro; IPR022711; RNase_Y_N.
DR   PANTHER; PTHR12826:SF15; PTHR12826:SF15; 1.
DR   Pfam; PF01966; HD; 1.
DR   Pfam; PF00013; KH_1; 1.
DR   Pfam; PF12072; RNase_Y_N; 1.
DR   SMART; SM00471; HDc; 1.
DR   SMART; SM00322; KH; 1.
DR   SUPFAM; SSF54791; SSF54791; 1.
DR   TIGRFAMs; TIGR00277; HDIG; 1.
DR   TIGRFAMs; TIGR03319; RNase_Y; 1.
DR   PROSITE; PS51831; HD; 1.
DR   PROSITE; PS50084; KH_TYPE_1; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Endonuclease; Hydrolase; Membrane; Nuclease;
KW   Reference proteome; RNA-binding; Transmembrane; Transmembrane helix.
FT   CHAIN           1..531
FT                   /note="Ribonuclease Y"
FT                   /id="PRO_0000163776"
FT   TRANSMEM        3..23
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00335"
FT   DOMAIN          221..281
FT                   /note="KH"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00335"
FT   DOMAIN          347..440
FT                   /note="HD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01175"
SQ   SEQUENCE   531 AA;  60317 MW;  8C918FB4738857C2 CRC64;
     MDIISIVLLV LLVVLVVAFI FYAQNIKKNK QDAESLFNEA ENKANEVMAN AKREAESLKM
     EAEAFKKEAR YTLREEEQKQ RREIEDEFKQ ERQELKETEK RLKQREEILD RKDDTLTKKE
     ENLDSKEENL VRKTDTLSKR EEQLAHIEEK KRLELERISN LSTDDAREII LSETRDGLTK
     EMAQIIRQSE EKAQVEADKR AKNIISLAIQ RVSSDSVAEQ TVSVVNLPDD GMKGRIIGRE
     GRNIRTFEAL TGIDVIIDDT PEAVVLSGFD PIRREIARMT LEQLVQDGRI HPARIEELVE
     KNRKALDHKM REYGEQAAFE VGAHNLHPDL MKIMGRLHFR TSYGQNVLDH SVEVAHIAGN
     LAGEMGENVA LAKRAGFLHD IGKALDHEVE GSHVEIGTEL ARKYKENPIV INTIASHHGD
     TEPLSNIAVL VAAADALSAA RPGARRESIE NYIKRLKDLE AISTSFEGVE TAFALQAGRE
     IRVMVKPEKL TDDQIVILAR DVKNRIEDEM DYPGNIKITV IRETRAIDYA K