RNZN_WHEAT
ID RNZN_WHEAT Reviewed; 176 AA.
AC P60193;
DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT 16-JAN-2004, sequence version 1.
DT 03-AUG-2022, entry version 73.
DE RecName: Full=Nuclear ribonuclease Z;
DE Short=RNase Z;
DE EC=3.1.26.11;
DE AltName: Full=Zinc phosphodiesterase ELAC;
DE AltName: Full=tRNA 3 endonuclease;
DE AltName: Full=tRNase Z;
DE Flags: Fragment;
GN Name=ELAC;
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Anderson O.D., Chao S., Choi D.W., Close T.J., Fenton R.D., Han P.S.,
RA Hsia C.C., Kang Y., Lazo G.R., Miller R., Rausch C.J., Seaton C.L.,
RA Tong J.C.;
RT "The structure and function of the expressed portion of the wheat
RT genomes.";
RL Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP PARTIAL PROTEIN SEQUENCE, FUNCTION, AND HOMODIMERIZATION.
RX PubMed=12032089; DOI=10.1093/emboj/21.11.2769;
RA Schiffer S., Roesch S., Marchfelder A.;
RT "Assigning a function to a conserved group of proteins: the tRNA 3'
RT - processing enzymes.";
RL EMBO J. 21:2769-2777(2002).
CC -!- FUNCTION: Zinc phosphodiesterase, which displays some tRNA 3'-
CC processing endonuclease activity. Probably involved in tRNA maturation,
CC by removing a 3'-trailer from precursor tRNA.
CC {ECO:0000269|PubMed:12032089}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage of RNA, removing extra 3' nucleotides
CC from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy
CC group is left at the tRNA terminus and a 5'-phosphoryl group is left
CC at the trailer molecule.; EC=3.1.26.11;
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000305};
CC -!- SUBUNIT: Homodimer.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the RNase Z family. {ECO:0000305}.
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DR EMBL; BE403456; -; NOT_ANNOTATED_CDS; mRNA.
DR AlphaFoldDB; P60193; -.
DR SMR; P60193; -.
DR STRING; 4565.Traes_6DS_8CB5C0B1E.1; -.
DR PRIDE; P60193; -.
DR EnsemblPlants; TraesCAD_scaffold_031803_01G000400.1; TraesCAD_scaffold_031803_01G000400.1; TraesCAD_scaffold_031803_01G000400.
DR EnsemblPlants; TraesCLE_scaffold_110265_01G000200.1; TraesCLE_scaffold_110265_01G000200.1; TraesCLE_scaffold_110265_01G000200.
DR EnsemblPlants; TraesPAR_scaffold_030934_01G000200.1; TraesPAR_scaffold_030934_01G000200.1; TraesPAR_scaffold_030934_01G000200.
DR EnsemblPlants; TraesROB_scaffold_070922_01G000300.1; TraesROB_scaffold_070922_01G000300.1; TraesROB_scaffold_070922_01G000300.
DR EnsemblPlants; TraesWEE_scaffold_035956_01G000200.1; TraesWEE_scaffold_035956_01G000200.1; TraesWEE_scaffold_035956_01G000200.
DR Gramene; TraesCAD_scaffold_031803_01G000400.1; TraesCAD_scaffold_031803_01G000400.1; TraesCAD_scaffold_031803_01G000400.
DR Gramene; TraesCLE_scaffold_110265_01G000200.1; TraesCLE_scaffold_110265_01G000200.1; TraesCLE_scaffold_110265_01G000200.
DR Gramene; TraesPAR_scaffold_030934_01G000200.1; TraesPAR_scaffold_030934_01G000200.1; TraesPAR_scaffold_030934_01G000200.
DR Gramene; TraesROB_scaffold_070922_01G000300.1; TraesROB_scaffold_070922_01G000300.1; TraesROB_scaffold_070922_01G000300.
DR Gramene; TraesWEE_scaffold_035956_01G000200.1; TraesWEE_scaffold_035956_01G000200.1; TraesWEE_scaffold_035956_01G000200.
DR eggNOG; ENOG502QVD0; Eukaryota.
DR BRENDA; 3.1.26.11; 6500.
DR Proteomes; UP000019116; Unplaced.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0042781; F:3'-tRNA processing endoribonuclease activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 3.60.15.10; -; 1.
DR InterPro; IPR001279; Metallo-B-lactamas.
DR InterPro; IPR036866; RibonucZ/Hydroxyglut_hydro.
DR Pfam; PF12706; Lactamase_B_2; 1.
DR SUPFAM; SSF56281; SSF56281; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Endonuclease; Hydrolase; Metal-binding;
KW Nuclease; Nucleus; Reference proteome; tRNA processing; Zinc.
FT CHAIN <1..>176
FT /note="Nuclear ribonuclease Z"
FT /id="PRO_0000155835"
FT NON_TER 1
FT NON_TER 176
SQ SEQUENCE 176 AA; 20004 MW; E5FE4242101CC031 CRC64;
GKAKPRLEIE GYPVEGISIG GHETCVIFPT LSLAFDIGRC PQRAVAQDFL FISHAHLDHI
GGLPMYVATR GLYRLRPPTI FVPKYLRELV ERLFDVHRAM DQSELNHTLV PLDIGEEYEL
RRDLKVRAFK TYHTIPSQGY VIYSVKQKLK QDYLGLPGSE IKRLKLSGVE ITNTVT
