RO60_CAEEL
ID RO60_CAEEL Reviewed; 643 AA.
AC Q27274; Q17933;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=RNA-binding protein RO60 {ECO:0000250|UniProtKB:P10155};
DE AltName: Full=60 kDa SS-A/Ro ribonucleoprotein homolog {ECO:0000305};
GN Name=rop-1 {ECO:0000312|WormBase:C12D8.11};
GN ORFNames=C12D8.11 {ECO:0000312|WormBase:C12D8.11};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=8566782; DOI=10.1016/0378-1119(95)00695-8;
RA Labbe J.C., Jannatipour M., Rokeach L.A.;
RT "The Caenorhabditis elegans rop-1 gene encodes the homologue of the human
RT 60-kDa Ro autoantigen.";
RL Gene 167:227-231(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7489501;
RA Van Horn D.J., Eisenberg D., O'Brien C.A., Wolin S.L.;
RT "Caenorhabditis elegans embryos contain only one major species of Ro RNP.";
RL RNA 1:293-303(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- FUNCTION: RNA-binding protein that binds to misfolded non-coding RNAs,
CC pre-5S rRNA, and several small cytoplasmic RNA molecules known as Y
CC RNAs. {ECO:0000250|UniProtKB:P42700}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P10155}.
CC -!- DOMAIN: The horseshoe-shaped TROVE domain is built with 7 helical HEAT-
CC like repeats, and is closed by the VWFA-like domain giving rise to a
CC ring-shaped monomer. Single-stranded RNA is bound in the positively
CC charged central cavity (By similarity). {ECO:0000250|UniProtKB:P42700}.
CC -!- DOMAIN: The MIDAS-like motif in the VWFA-like domain binds divalent
CC metal cations. {ECO:0000250|UniProtKB:P42700}.
CC -!- SIMILARITY: Belongs to the Ro 60 kDa family. {ECO:0000305}.
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DR EMBL; U21487; AAA96949.1; -; Genomic_DNA.
DR EMBL; L41729; AAC41575.1; -; mRNA.
DR EMBL; Z73969; CAA98241.1; -; Genomic_DNA.
DR EMBL; AL021479; CAA98241.1; JOINED; Genomic_DNA.
DR PIR; T19225; T19225.
DR RefSeq; NP_505638.1; NM_073237.6.
DR AlphaFoldDB; Q27274; -.
DR SMR; Q27274; -.
DR BioGRID; 44455; 15.
DR IntAct; Q27274; 1.
DR STRING; 6239.C12D8.11; -.
DR iPTMnet; Q27274; -.
DR EPD; Q27274; -.
DR PaxDb; Q27274; -.
DR PeptideAtlas; Q27274; -.
DR EnsemblMetazoa; C12D8.11.1; C12D8.11.1; WBGene00004405.
DR GeneID; 179425; -.
DR KEGG; cel:CELE_C12D8.11; -.
DR UCSC; C12D8.11.1; c. elegans.
DR CTD; 179425; -.
DR WormBase; C12D8.11; CE15613; WBGene00004405; rop-1.
DR eggNOG; KOG4465; Eukaryota.
DR GeneTree; ENSGT00390000006200; -.
DR HOGENOM; CLU_024421_1_0_1; -.
DR InParanoid; Q27274; -.
DR OMA; WWYEWLK; -.
DR OrthoDB; 472016at2759; -.
DR PhylomeDB; Q27274; -.
DR SignaLink; Q27274; -.
DR PRO; PR:Q27274; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00004405; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:1990904; C:ribonucleoprotein complex; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR Gene3D; 3.40.50.410; -; 2.
DR InterPro; IPR040322; TROVE2.
DR InterPro; IPR008858; TROVE_dom.
DR InterPro; IPR037214; TROVE_dom_sf.
DR InterPro; IPR036465; vWFA_dom_sf.
DR PANTHER; PTHR14202; PTHR14202; 1.
DR Pfam; PF05731; TROVE; 1.
DR SUPFAM; SSF140864; SSF140864; 1.
DR SUPFAM; SSF53300; SSF53300; 1.
DR PROSITE; PS50988; TROVE; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Metal-binding; Reference proteome; Repeat; Ribonucleoprotein;
KW RNA-binding.
FT CHAIN 1..643
FT /note="RNA-binding protein RO60"
FT /id="PRO_0000174172"
FT DOMAIN 63..473
FT /note="TROVE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00343"
FT REGION 186..390
FT /note="RNA-binding"
FT /evidence="ECO:0000250|UniProtKB:P42700"
FT REGION 465..643
FT /note="VWFA-like domain"
FT /evidence="ECO:0000250|UniProtKB:P42700"
FT BINDING 482
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000250|UniProtKB:P42700"
FT BINDING 484
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000250|UniProtKB:P42700"
FT BINDING 549
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000250|UniProtKB:P42700"
SQ SEQUENCE 643 AA; 72842 MW; FDE65BF3F37386B7 CRC64;
MADELNEFQE AGNFNEEALM RLSNVCARLR RMQMLESDVE ITVVDGELKR VPRQMEKVKD
GQVENNAGGF VFPVSDETQV RRFLILGSDK GSYHQSSEKI TIDNAQRIIK IIEQGNGHMV
LKELALINAE NRNPKMNAMI FTLAICARIS THDTTKKTEC PMLNAYSDYI RALHDSALDL
IPEVCRTPTH LFEFVDYCQT ISESTKAGGA KSSTGWGRSM RNAISKWYTT KTTEKLAMLL
TKYPQREGWS HRDLFRLAHP NLMDSRSHGQ SEDRLEREQL FRFAVKGDLV KRKRKMSVEE
VAEVEKVWDK KALKLPYTEE QLIKEEQSRA LNLVEAYLKL KNEQSEEVIV AAIKKHGLVR
EHLPTTSLNS KLVWETLFDV SMPMTAMIRN LAKMTVVGAL DEKRVDNIVK RLTDQEELRR
SRIHPINLLT ARAVYAQGRG DKGSLTWEPN QKICDALEAG FYKAFVNAPP TGKRYCLALD
VSGSMTSRVS SSPLSCREAA TGMSLINLHN EAEVRCVAFC DKLTELPFTK DWKIGQVNDY
VNNLDFGRTD CGLPMTWATE NNLKFDVFII YTDNDTWAGE IHPFEAIKKY REASGIHDAK
VIVMAMQAYD YSIADPSDAG MLDITGFDSA VPQIVHEFVT GKI
