ID   ROAA_EUGGR              Reviewed;         516 AA.
AC   P30397; Q95AL4;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   25-MAY-2022, entry version 54.
DE   RecName: Full=Ribosomal operon-associated A protein;
DE            Short=RoaA;
GN   Name=roaA; Synonyms=ycf82;
OS   Euglena gracilis.
OG   Plastid; Chloroplast.
OC   Eukaryota; Discoba; Euglenozoa; Euglenida; Spirocuta; Euglenophyceae;
OC   Euglenales; Euglenaceae; Euglena.
OX   NCBI_TaxID=3039;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Z / UTEX 753;
RX   PubMed=8346031; DOI=10.1093/nar/21.15.3537;
RA   Hallick R.B., Hong L., Drager R.G., Favreau M.R., Monfort A., Orsat B.,
RA   Spielmann A., Stutz E.;
RT   "Complete sequence of Euglena gracilis chloroplast DNA.";
RL   Nucleic Acids Res. 21:3537-3544(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2), AND DETECTION OF THE
RP   PROTEIN.
RC   STRAIN=Z / UTEX 753;
RX   PubMed=7489521;
RA   Jenkins K.P., Hong L., Hallick R.B.;
RT   "Alternative splicing of the Euglena gracilis chloroplast roaA
RT   transcript.";
RL   RNA 1:624-633(1995).
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC         Comment=Isoform 1 is the more common.;
CC       Name=1;
CC         IsoId=P30397-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=P30397-2; Sequence=VSP_009646;
CC   -!- MISCELLANEOUS: The protein migrates at 80 kDa in SDS-PAGE gels.
CC   -!- SIMILARITY: Belongs to the roaA family. {ECO:0000305}.
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DR   EMBL; X70810; CAA50104.1; -; Genomic_DNA.
DR   EMBL; X70810; CAC69147.1; -; Genomic_DNA.
DR   EMBL; Z11874; CAA77921.1; -; Genomic_DNA.
DR   PIR; S34892; S34525.
DR   RefSeq; NP_041917.1; NC_001603.2. [P30397-1]
DR   AlphaFoldDB; P30397; -.
DR   SMR; P30397; -.
DR   GeneID; 1457325; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   InterPro; IPR025960; RVT_N.
DR   Pfam; PF13655; RVT_N; 1.
PE   3: Inferred from homology;
KW   Alternative splicing; Chloroplast; Plastid.
FT   CHAIN           1..516
FT                   /note="Ribosomal operon-associated A protein"
FT                   /id="PRO_0000217293"
FT   VAR_SEQ         17..18
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_009646"
SQ   SEQUENCE   516 AA;  64334 MW;  E2079EF48423490A CRC64;
     MFSFFYRFNW NFIKWNLVDN TVSLTQHKIY LASKKSDIFL VKNKQRRFFK SFYAHIFSVR
     NCFEKFPSLQ SYFYSSKDKF FLVSLLSSKR NLFLHGFFYN IKKAFLFYSY DFFLLYKLSF
     SLLLTSTLLP YINDINPCFI KDYYFFVEGN FFDFEQILID VFKYSYYFKV ENFKFLYFAR
     FSWIYKNFPL DVNFLKNFLD RKNLVFFKSL FLIIFNFIFN GLNFFFQENF FISYKVYYLF
     YIDKFYFFFK FPYDFFICKK IIISFFSLRG IILNININSF YRFETFVFNF FIFDFLRFGS
     NILLHINKKH IQFYKLSLKM IVKMFLKKSV FFLVNLLNKK ILDCLNRGFF CFNNNFLFLE
     LDLYLYRLLW RYIKKLHSRK TRTWIYSKYW KFFSGIWRFF ITDIKTGNFL FLKSHLYSSK
     YFYSYRNMKF KISNTLNIFN LYNKGKLERM IFEKFKYKFS PNFIVLYNNQ RGLCFFCKKS
     IYSNRFVILN IKKWNFSFFE NLILIHFYCN NFNQLQ