ID   ROB1_RAT                Reviewed;         240 AA.
AC   O55006;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Protein RoBo-1;
DE   AltName: Full=Rodent bone protein;
DE   Flags: Precursor;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, TISSUE SPECIFICITY, AND
RP   GLYCOSYLATION.
RC   STRAIN=CD Charles River; TISSUE=Tibial bone;
RX   PubMed=9461570; DOI=10.1074/jbc.273.7.3878;
RA   Noel L.S., Champion B.R., Holley C.L., Simmons C.J., Morris D.C.,
RA   Payne J.A., Lean J.M., Chambers T.J., Zaman G., Lanyon L.E., Suva L.J.,
RA   Miller L.R.;
RT   "RoBo-1, a novel member of the urokinase plasminogen activator
RT   receptor/CD59/Ly-6/snake toxin family selectively expressed in rat bone and
RT   growth plate cartilage.";
RL   J. Biol. Chem. 273:3878-3883(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Brown Norway;
RA   Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: May play a novel role in the growth or remodeling of bone.
CC       {ECO:0000269|PubMed:9461570}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed abundantly in bone, including the
CC       lengthening growth plate where cartilage is remodeled into bone.
CC       {ECO:0000269|PubMed:9461570}.
CC   -!- INDUCTION: Up-regulated by estradiol. {ECO:0000269|PubMed:9461570}.
CC   -!- PTM: N-glycosylated. {ECO:0000269|PubMed:9461570}.
CC   -!- SIMILARITY: Belongs to the CNF-like-inhibitor family. {ECO:0000305}.
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DR   EMBL; AF041083; AAC40033.1; -; mRNA.
DR   EMBL; CH473948; EDM04544.1; -; Genomic_DNA.
DR   RefSeq; NP_113725.1; NM_031537.1.
DR   RefSeq; XP_006246439.1; XM_006246377.3.
DR   AlphaFoldDB; O55006; -.
DR   SMR; O55006; -.
DR   GlyGen; O55006; 2 sites.
DR   PaxDb; O55006; -.
DR   PRIDE; O55006; -.
DR   Ensembl; ENSRNOT00000003797; ENSRNOP00000003797; ENSRNOG00000002820.
DR   GeneID; 24906; -.
DR   KEGG; rno:24906; -.
DR   RGD; 3683; LOC24906.
DR   eggNOG; ENOG502SDSC; Eukaryota.
DR   GeneTree; ENSGT00730000111648; -.
DR   InParanoid; O55006; -.
DR   OMA; PNGVECP; -.
DR   OrthoDB; 1485195at2759; -.
DR   PhylomeDB; O55006; -.
DR   TreeFam; TF339495; -.
DR   PRO; PR:O55006; -.
DR   Proteomes; UP000002494; Chromosome 10.
DR   Proteomes; UP000234681; Chromosome 10.
DR   Bgee; ENSRNOG00000002820; Expressed in spleen and 16 other tissues.
DR   ExpressionAtlas; O55006; baseline and differential.
DR   Genevisible; O55006; RN.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0046849; P:bone remodeling; IEP:UniProtKB.
DR   Gene3D; 2.10.60.10; -; 1.
DR   InterPro; IPR016054; LY6_UPA_recep-like.
DR   InterPro; IPR045860; Snake_toxin-like_sf.
DR   Pfam; PF00021; UPAR_LY6; 1.
DR   SUPFAM; SSF57302; SSF57302; 1.
PE   1: Evidence at protein level;
KW   Disulfide bond; Glycoprotein; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..240
FT                   /note="Protein RoBo-1"
FT                   /id="PRO_0000402834"
FT   CARBOHYD        42
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        153
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        47..76
FT                   /evidence="ECO:0000250"
FT   DISULFID        81..102
FT                   /evidence="ECO:0000250"
FT   DISULFID        103..108
FT                   /evidence="ECO:0000250"
FT   DISULFID        127..151
FT                   /evidence="ECO:0000250"
FT   DISULFID        144..171
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   240 AA;  26187 MW;  72D746C0284ACAFB CRC64;
     MSWFLVLKCL LTVCIISHLS VSSTESYGCI RKTCFGGRCL HNTTRSCEFS KGCFSQLQEF
     AVPLLLLNRR VEQRGCSEDN CTELAFSATL GIDWMFSYNH QCCYSEQCNN KPINVSPLSL
     QPNGVECPTC YSELGTCRPV SLKCTGAQTT CVNVTGQGIR EDFIKIHAMG CATQTACNLK
     NVIILNNIKI DTSCVSGSPP LRYSPSLSTD QKTSSATAPT LCLLAAVLPA IMVMESFSEL