ROB_ECOLI
ID ROB_ECOLI Reviewed; 289 AA.
AC P0ACI0; P27292; Q2M5S1;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 1.
DT 25-MAY-2022, entry version 125.
DE RecName: Full=Right origin-binding protein;
GN Name=rob; OrderedLocusNames=b4396, JW4359;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-21.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8449900; DOI=10.1016/s0021-9258(18)53330-5;
RA Skarstad K., Thoeny B., Hwang D.S., Kornberg A.;
RT "A novel binding protein of the origin of the Escherichia coli
RT chromosome.";
RL J. Biol. Chem. 268:5365-5370(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT from 92.8 through 100 minutes.";
RL Nucleic Acids Res. 23:2105-2119(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- FUNCTION: Binds to the right arm of the replication origin oriC of the
CC chromosome. Rob binding may influence the formation of the
CC nucleoprotein structure, required for oriC function in the initiation
CC of replication.
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DR EMBL; M97495; AAA24569.1; -; Genomic_DNA.
DR EMBL; U14003; AAA97292.1; -; Genomic_DNA.
DR EMBL; U00096; AAC77349.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE78385.1; -; Genomic_DNA.
DR PIR; JU0158; JU0158.
DR RefSeq; NP_418813.1; NC_000913.3.
DR RefSeq; WP_000371666.1; NZ_STEB01000033.1.
DR PDB; 1D5Y; X-ray; 2.70 A; A/B/C/D=3-289.
DR PDBsum; 1D5Y; -.
DR AlphaFoldDB; P0ACI0; -.
DR SMR; P0ACI0; -.
DR BioGRID; 4259321; 108.
DR DIP; DIP-47868N; -.
DR IntAct; P0ACI0; 17.
DR STRING; 511145.b4396; -.
DR jPOST; P0ACI0; -.
DR PaxDb; P0ACI0; -.
DR PRIDE; P0ACI0; -.
DR EnsemblBacteria; AAC77349; AAC77349; b4396.
DR EnsemblBacteria; BAE78385; BAE78385; BAE78385.
DR GeneID; 66671716; -.
DR GeneID; 948916; -.
DR KEGG; ecj:JW4359; -.
DR KEGG; eco:b4396; -.
DR PATRIC; fig|1411691.4.peg.2288; -.
DR EchoBASE; EB1340; -.
DR eggNOG; COG2207; Bacteria.
DR eggNOG; COG3708; Bacteria.
DR HOGENOM; CLU_000445_81_1_6; -.
DR InParanoid; P0ACI0; -.
DR OMA; WRQYLGE; -.
DR PhylomeDB; P0ACI0; -.
DR BioCyc; EcoCyc:PD04418; -.
DR EvolutionaryTrace; P0ACI0; -.
DR PRO; PR:P0ACI0; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:EcoliWiki.
DR Gene3D; 3.20.80.10; -; 1.
DR InterPro; IPR010499; AraC_E-bd.
DR InterPro; IPR029442; GyrI-like.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR018060; HTH_AraC.
DR InterPro; IPR018062; HTH_AraC-typ_CS.
DR InterPro; IPR011256; Reg_factor_effector_dom_sf.
DR InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR Pfam; PF06445; GyrI-like; 1.
DR Pfam; PF12833; HTH_18; 1.
DR PRINTS; PR00032; HTHARAC.
DR SMART; SM00871; AraC_E_bind; 1.
DR SMART; SM00342; HTH_ARAC; 1.
DR SUPFAM; SSF46689; SSF46689; 2.
DR SUPFAM; SSF55136; SSF55136; 1.
DR PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; DNA-binding; Reference proteome.
FT CHAIN 1..289
FT /note="Right origin-binding protein"
FT /id="PRO_0000194578"
FT DOMAIN 8..106
FT /note="HTH araC/xylS-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT DNA_BIND 25..46
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT DNA_BIND 73..96
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT HELIX 4..15
FT /evidence="ECO:0007829|PDB:1D5Y"
FT STRAND 18..21
FT /evidence="ECO:0007829|PDB:1D5Y"
FT HELIX 25..28
FT /evidence="ECO:0007829|PDB:1D5Y"
FT TURN 29..31
FT /evidence="ECO:0007829|PDB:1D5Y"
FT HELIX 35..46
FT /evidence="ECO:0007829|PDB:1D5Y"
FT HELIX 50..68
FT /evidence="ECO:0007829|PDB:1D5Y"
FT HELIX 73..79
FT /evidence="ECO:0007829|PDB:1D5Y"
FT HELIX 85..96
FT /evidence="ECO:0007829|PDB:1D5Y"
FT HELIX 100..105
FT /evidence="ECO:0007829|PDB:1D5Y"
FT STRAND 107..110
FT /evidence="ECO:0007829|PDB:1D5Y"
FT STRAND 128..133
FT /evidence="ECO:0007829|PDB:1D5Y"
FT STRAND 136..144
FT /evidence="ECO:0007829|PDB:1D5Y"
FT HELIX 149..151
FT /evidence="ECO:0007829|PDB:1D5Y"
FT HELIX 152..170
FT /evidence="ECO:0007829|PDB:1D5Y"
FT STRAND 178..186
FT /evidence="ECO:0007829|PDB:1D5Y"
FT STRAND 188..190
FT /evidence="ECO:0007829|PDB:1D5Y"
FT STRAND 194..203
FT /evidence="ECO:0007829|PDB:1D5Y"
FT HELIX 204..207
FT /evidence="ECO:0007829|PDB:1D5Y"
FT HELIX 208..210
FT /evidence="ECO:0007829|PDB:1D5Y"
FT STRAND 215..219
FT /evidence="ECO:0007829|PDB:1D5Y"
FT STRAND 222..232
FT /evidence="ECO:0007829|PDB:1D5Y"
FT HELIX 233..235
FT /evidence="ECO:0007829|PDB:1D5Y"
FT HELIX 236..245
FT /evidence="ECO:0007829|PDB:1D5Y"
FT HELIX 247..250
FT /evidence="ECO:0007829|PDB:1D5Y"
FT STRAND 260..264
FT /evidence="ECO:0007829|PDB:1D5Y"
FT HELIX 266..268
FT /evidence="ECO:0007829|PDB:1D5Y"
FT STRAND 278..288
FT /evidence="ECO:0007829|PDB:1D5Y"
SQ SEQUENCE 289 AA; 33145 MW; 4436B1A04BE86A93 CRC64;
MDQAGIIRDL LIWLEGHLDQ PLSLDNVAAK AGYSKWHLQR MFKDVTGHAI GAYIRARRLS
KSAVALRLTA RPILDIALQY RFDSQQTFTR AFKKQFAQTP ALYRRSPEWS AFGIRPPLRL
GEFTMPEHKF VTLEDTPLIG VTQSYSCSLE QISDFRHEMR YQFWHDFLGN APTIPPVLYG
LNETRPSQDK DDEQEVFYTT ALAQDQADGY VLTGHPVMLQ GGEYVMFTYE GLGTGVQEFI
LTVYGTCMPM LNLTRRKGQD IERYYPAEDA KAGDRPINLR CELLIPIRR
