ROC10_DICDI
ID ROC10_DICDI Reviewed; 2646 AA.
AC Q6XHA6; Q54E99;
DT 20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Probable inactive serine/threonine-protein kinase roco10;
DE AltName: Full=Ras of complex proteins and C-terminal of roc 10;
GN Name=roco10; ORFNames=DDB_G0291710;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=14654223; DOI=10.1016/j.bbamcr.2003.08.008;
RA Bosgraaf L., van Haastert P.J.M.;
RT "Roc, a Ras/GTPase domain in complex proteins.";
RL Biochim. Biophys. Acta 1643:5-10(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- DOMAIN: The protein kinase domain is predicted to be catalytically
CC inactive.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. TKL Ser/Thr
CC protein kinase family. ROCO subfamily. {ECO:0000305}.
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DR EMBL; AY232272; AAO83655.1; -; Genomic_DNA.
DR EMBL; AAFI02000181; EAL61591.1; -; Genomic_DNA.
DR RefSeq; XP_630003.1; XM_630001.1.
DR AlphaFoldDB; Q6XHA6; -.
DR SMR; Q6XHA6; -.
DR STRING; 44689.DDB0201665; -.
DR PaxDb; Q6XHA6; -.
DR EnsemblProtists; EAL61591; EAL61591; DDB_G0291710.
DR GeneID; 8628293; -.
DR KEGG; ddi:DDB_G0291710; -.
DR dictyBase; DDB_G0291710; roco10.
DR eggNOG; KOG0192; Eukaryota.
DR eggNOG; KOG0619; Eukaryota.
DR HOGENOM; CLU_227627_0_0_1; -.
DR InParanoid; Q6XHA6; -.
DR OMA; HLMIRKM; -.
DR PRO; PR:Q6XHA6; -.
DR Proteomes; UP000002195; Chromosome 6.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
DR GO; GO:0004672; F:protein kinase activity; IEA:InterPro.
DR GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR Gene3D; 1.10.167.10; -; 1.
DR Gene3D; 1.10.555.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR011043; Gal_Oxase/kelch_b-propeller.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR016137; RGS.
DR InterPro; IPR036305; RGS_sf.
DR InterPro; IPR044926; RGS_subdomain_2.
DR InterPro; IPR008936; Rho_GTPase_activation_prot.
DR InterPro; IPR000198; RhoGAP_dom.
DR InterPro; IPR020859; ROC_dom.
DR InterPro; IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
DR Pfam; PF13855; LRR_8; 2.
DR Pfam; PF07714; PK_Tyr_Ser-Thr; 1.
DR Pfam; PF00615; RGS; 1.
DR Pfam; PF00620; RhoGAP; 1.
DR PRINTS; PR01301; RGSPROTEIN.
DR SMART; SM00369; LRR_TYP; 9.
DR SMART; SM00315; RGS; 1.
DR SMART; SM00324; RhoGAP; 1.
DR SUPFAM; SSF48097; SSF48097; 1.
DR SUPFAM; SSF48350; SSF48350; 1.
DR SUPFAM; SSF50965; SSF50965; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS51450; LRR; 11.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS50132; RGS; 1.
DR PROSITE; PS50238; RHOGAP; 1.
DR PROSITE; PS51424; ROC; 1.
PE 3: Inferred from homology;
KW ATP-binding; GTPase activation; Leucine-rich repeat; Nucleotide-binding;
KW Reference proteome; Repeat.
FT CHAIN 1..2646
FT /note="Probable inactive serine/threonine-protein kinase
FT roco10"
FT /id="PRO_0000358896"
FT DOMAIN 585..807
FT /note="Rho-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00172"
FT REPEAT 968..987
FT /note="LRR 1"
FT REPEAT 989..1011
FT /note="LRR 2"
FT REPEAT 1012..1033
FT /note="LRR 3"
FT REPEAT 1040..1061
FT /note="LRR 4"
FT REPEAT 1062..1083
FT /note="LRR 5"
FT REPEAT 1085..1108
FT /note="LRR 6"
FT REPEAT 1109..1131
FT /note="LRR 7"
FT REPEAT 1132..1154
FT /note="LRR 8"
FT REPEAT 1155..1176
FT /note="LRR 9"
FT REPEAT 1178..1199
FT /note="LRR 10"
FT REPEAT 1201..1222
FT /note="LRR 11"
FT REPEAT 1224..1247
FT /note="LRR 12"
FT REPEAT 1248..1270
FT /note="LRR 13"
FT DOMAIN 1262..1474
FT /note="Roc"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00758"
FT REPEAT 1271..1298
FT /note="LRR 14"
FT REPEAT 1303..1327
FT /note="LRR 15"
FT DOMAIN 2049..2342
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT DOMAIN 2412..2536
FT /note="RGS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00171"
FT REGION 28..122
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 138..168
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 205..248
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 281..457
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 477..516
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 605..656
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 882..907
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1293..1317
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1651..1670
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1957..2026
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 28..55
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 56..70
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 71..122
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 210..248
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 281..456
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 483..516
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1957..1995
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1996..2026
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 2055..2063
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 2094
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
SQ SEQUENCE 2646 AA; 293356 MW; 694BF1EFBC81BEF9 CRC64;
MSGLLDDDEI IQVSPDSIIT SSIGSASLNY SVNGADDNKS IPPSSPQQNL LENDTLSPPP
PLPTQTINTS SSPPPPTIIT TTTTTTTTTA MSPVATTTTS SPTISASPTY ITSPSGTRLS
GGNTFNAQQL KSLLLASNIP IPPVPTSPTS TPPNLQTNNN NKQDKDKDNE TFATLINEEL
PLTTEEKENI KAQKKDLISS LPEVPLFISP SPTLSRNNNG KRNSGGNKPP LSPIKISSTS
AAGDVSPSIS LNNSGSGIYN STSPSGTAST QTLNILSQLQ QQQRNGNNNN NNNNNNNYLS
SPTMPALNKH STHGNFHNSV AGYGNNNNNN NNNNKQPQHP MNGNHSPSNG TSGSLSMSGS
GIDNGGNNNN NSNTHGSSSN QSSGVTSPII QSTSPQPPHL TGLNSDSQMP LSSSPTMGYP
RGNHNKKTSA SAVSSQNRSP LMNSTGVSSS SSGVKQAGAG EKVMQFLGKM SLSKERMSKL
VSQSKEQYKT PSSPYQQSTS SSISSGSGTI SGHTSPNSLN SVQVVPFEGI FGIPLRVILR
YPNESGNQIP SILNSIFTIL ESSSALSTNR LFYGDYNTST SNLASSISPI STAANTTIPT
ISVPTSPNLS SSLSSSSSSG SSGNSSPNII NQLQQQQQPQ PTTTTTTNTN TSPHQYLSQK
SIKLEVENIC NQYDQGIEVS LKKCNVHVVS EILIEFFNRL PEPIISVEFY ETLLNYYQTE
TLLHLMIRKM NNPNKSILCR LMKYLKDIMV YTNFNDVTLE LLVERFHKFI LRPTIGTIAQ
SKEISESHLK TIKDIVTMFI QQADILFQSP EERMNTDDAQ ILYIEEVLTS TQKSQHQSSV
GNLWLFHKQI VWRPKVNIDH GEPDLAILFS SILKIVLYTP PQSSSSSSSS SISNSSSASS
SSSSTPSISS TSLSSSLKVL PNFTVYCSSD TKTVITFSFQ SPSTCKLIYA YINSVTKSIS
QLNRIIPQKL DMFSLELESL PNEIKQLKDL QELNLNRNKF KLLPGDLARL TSLRTICIEE
NNLTEISSEM ADFLGTRLSN LENVTLSSNR LVVLPPLYTW LKLKTLNISN NYLTKLPIDI
FQIPTLEVLR VSNNDLDDNG IPKICTSTKL RSLDLRKNHL TSIPEGIINL VELQVLTLAD
NQISHLTSDI QKLTSLTELN LNGNQIQSLP PQLLLLTNLK KLYLDNNQLQ SISSAIHRMQ
SLIELRLTNN NISRLPPGIV ALKKLNSLEL TGNKPLKDNI PEKYIQKGKE GIFSFFSETM
RTNVPCYRTR IIMLGDKSTG KSNLIKCLKK LPKSSFSSSS SNLPSLNNLN SNNSNNSGNS
KTNILDIQDW QCPINVDGPD GKKKKTITIH LWEFEGMQND ISHVFFVPNI IYTVCFNLSK
FGSSKSNEQK ITSYLHGINN YDKKATIIII GTHLDEISSN SKKQVDKVFD CLSLKYKQLF
PTLNLVFHAV STLKSDADGI RKLRRDIKQM IAKNPILKQT YPASFMFLED YLKEESNLMS
PPLVTKKTLQ QMARTMELHN EPHFTQLKSL FNSLGSIALF EQFIRMEPGS TPQKTEMIAL
NPIWIAKAIA SLVCFNPQNL PFNVSVSAEE ANDASHDSTI TGILPHRVLR YVWGTNSKYY
VPERFFSLFL SLLESNDLAI NIYSLEVNNN NNNNSNGNNV GRGRSGSRSM SIHEANRSNS
YFGVNLNNTS SLSSFTSNQK VRNGRSSSFN ARVGWALVSS LLAPPPQLSI NGNCSISSNS
SSSSSSLSNA LNLNNISSMI PIIDLPGIWE AFPESPEIHQ FSRRFSLDVI PNGLFGRLLS
RLMQHAHLYK CWKDLAILVP EIASMSTTSS SSSSSTANST TTTSATTLQS LASGSSNNTL
QQQFREERIL VNVDHDSNTI EITIRFIRPS TLSSQVYSIF ESLVSKWYKV SFKTFIPCSH
CIEKKIVTPH LFKLEECEAQ IFKGELGIYC QYKPNNNNSS NDTSSPIASS RSNPKISRKD
SKNLIQSNNN DNNNSLSKKD LKELAKQNKE KEKEKEKDKD KEKEKERKVE IYRDDSFLVP
IRSLLLDQFS ICHFIKEIDY REIEVLPDHS NQNENGTSTQ SMMGMYGKQF VNIKVFNAPL
LGADFQNNCA RILSSFRHEA LSLYNFRHSN VLSIIGISMN PIAIITENPT FGSKGSTTLS
EYIQDRQKHP EIPWNIKLKI ALDIARAMDK LNSHSPPFLL TNLTSSGIIL EKSNDHQSSG
NGLEIEPFVN AKIIDFSQSS FLPSLFPTST TPKSYHSPEV LQKLNYHENS DVYSFGIILY
ELLTRSIAFQ DHDRFSNIVI SGQRPSIPLD CLPSFADLIK DCWSGEPLNR PSPSKIISQL
YTIKKEIESK EHSYKSLASD NNIYSDHPLP SGGSIIYQDK IIHFGGWNNS SKPHSKVFAL
NLSSMLFEDK LMVVLNNKQS TTYKAFYTHM QSEFNEENLM FYEAIKTFKS LPNQTPDDRE
IIKIQSKKIY QVFIGENAPK EINLPFLLKK ELKNKIDFPD GPSVTVFNDT LTFVISSIED
SFHRFKFTVP TTNKNGWVEI ECKGIPPLPM VGHSSILWNN SLIVIGGWFN KARLLNQIHI
LNLETFEWNQ FVCTGDIPPS SIAALNITLH GDYLLAYYER TDSIKQQIFR LSLDSFIWFS
LKLSNV
