ID   ROC2_AGRRH              Reviewed;         180 AA.
AC   P49408; Q7AJP6;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   25-MAY-2022, entry version 54.
DE   RecName: Full=Cytokinin-beta-glucosidase;
DE            EC=3.2.1.-;
DE   AltName: Full=ROL C protein;
GN   Name=rolC; ORFNames=riorf16;
OS   Agrobacterium rhizogenes.
OG   Plasmid pRi1724.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium.
OX   NCBI_TaxID=359;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=MAFF03-01724;
RX   PubMed=8159796; DOI=10.1104/pp.104.2.801;
RA   Kiyokawa S., Kobayashi K., Kikuchi Y., Kamada H., Harada H.;
RT   "Root-inducing region of mikimopine type Ri plasmid pRi1724.";
RL   Plant Physiol. 104:801-802(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=MAFF03-01724;
RX   PubMed=7764693; DOI=10.1271/bbb.58.548;
RA   Tanaka N., Ikeda T., Oka A.;
RT   "Nucleotide sequence of the rol region of the mikimopine-type root-inducing
RT   plasmid pRi1724.";
RL   Biosci. Biotechnol. Biochem. 58:548-551(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=MAFF03-01724;
RX   PubMed=11273700; DOI=10.1006/jmbi.2001.4488;
RA   Moriguchi K., Maeda Y., Satou M., Hardayani N.S.N., Kataoka M., Tanaka N.,
RA   Yoshida K.;
RT   "The complete nucleotide sequence of a plant root-inducing (Ri) plasmid
RT   indicates its chimeric structure and evolutionary relationship between
RT   tumor-inducing (Ti) and symbiotic (Sym) plasmids in Rhizobiaceae.";
RL   J. Mol. Biol. 307:771-784(2001).
CC   -!- FUNCTION: Hydrolyzes cytokinin glucosides thus liberating free
CC       cytokinins. Contributes to the root inducing activity.
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DR   EMBL; X64255; CAA45541.1; -; Genomic_DNA.
DR   EMBL; AB006689; BAA22336.1; -; Genomic_DNA.
DR   EMBL; AP002086; BAB16135.1; -; Genomic_DNA.
DR   PIR; JC2107; JC2107.
DR   RefSeq; NP_066597.1; NC_002575.1.
DR   RefSeq; WP_010900206.1; NZ_KY000038.1.
DR   AlphaFoldDB; P49408; -.
DR   SMR; P49408; -.
DR   GO; GO:0008422; F:beta-glucosidase activity; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0009691; P:cytokinin biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR006065; Glyco_hydro_41.
DR   InterPro; IPR006064; Glycosidase.
DR   Pfam; PF02027; RolB_RolC; 1.
DR   PRINTS; PR00746; GLHYDRLASE41.
PE   4: Predicted;
KW   Cytokinin biosynthesis; Glycosidase; Hydrolase; Plasmid.
FT   CHAIN           1..180
FT                   /note="Cytokinin-beta-glucosidase"
FT                   /id="PRO_0000097394"
FT   REGION          26..54
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   180 AA;  19846 MW;  F0EBE7865280F72C CRC64;
     MAEFDLCALF SSLKVGDVSS SDELKKHIQS ASKERTPLTE PGEGQSMDID EEGGRQDPGI
     LYLYVDCPTM MRCFYGGSLP YNSRHGALIT NLPPYQKDVS LGEVCRGLRQ ASGFFGYEDV
     IRSAYFAALS VPGYFVKLDG QMELTSTKGK SLTFDLYASN QLRLEPGALV RHGECKFGME