ROCK1_ARATH
ID ROCK1_ARATH Reviewed; 325 AA.
AC Q8LES0; A0JQ95; Q8VYH1; Q9LV78;
DT 21-MAR-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=UDP-N-acetylglucosamine transporter ROCK1 {ECO:0000305};
DE AltName: Full=CMP-sialic acid transporter 5 {ECO:0000305};
DE Short=CMP-SA-Tr 5 {ECO:0000305};
DE Short=CMP-Sia-Tr 5 {ECO:0000305};
DE AltName: Full=Protein REPRESSOR OF CYTOKININ DEFICIENCY 1 {ECO:0000303|PubMed:25535363};
GN Name=ROCK1 {ECO:0000303|PubMed:25535363};
GN OrderedLocusNames=At5g65000 {ECO:0000312|Araport:AT5G65000};
GN ORFNames=MXK3.23 {ECO:0000312|EMBL:BAA97312.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP GENE FAMILY.
RX PubMed=15456736; DOI=10.1093/glycob/cwh159;
RA Bakker H., Routier F., Oelmann S., Jordi W., Lommen A., Gerardy-Schahn R.,
RA Bosch D.;
RT "Molecular cloning of two Arabidopsis UDP-galactose transporters by
RT complementation of a deficient Chinese hamster ovary cell line.";
RL Glycobiology 15:193-201(2005).
RN [8]
RP GENE FAMILY.
RX PubMed=25053812; DOI=10.1073/pnas.1406073111;
RA Rautengarten C., Ebert B., Moreno I., Temple H., Herter T., Link B.,
RA Donas-Cofre D., Moreno A., Saez-Aguayo S., Blanco F., Mortimer J.C.,
RA Schultink A., Reiter W.D., Dupree P., Pauly M., Heazlewood J.L.,
RA Scheller H.V., Orellana A.;
RT "The Golgi localized bifunctional UDP-rhamnose/UDP-galactose transporter
RT family of Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:11563-11568(2014).
RN [9]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=25535363; DOI=10.1073/pnas.1419050112;
RA Niemann M.C., Bartrina I., Ashikov A., Weber H., Novak O., Spichal L.,
RA Strnad M., Strasser R., Bakker H., Schmuelling T., Werner T.;
RT "Arabidopsis ROCK1 transports UDP-GlcNAc/UDP-GalNAc and regulates ER
RT protein quality control and cytokinin activity.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:291-296(2015).
CC -!- FUNCTION: Mediates the transport of UDP-linked acetylated hexosamines
CC across the endoplasmic reticulum (ER) membrane (PubMed:25535363).
CC Facilitates UDP-N-acetylglucosamine (UDP-GlcNAc) and UDP-N-
CC acetylgalactosamine (UDP-GalNAc) transport (PubMed:25535363). Regulates
CC the cytokinin signal in meristematic cells through modulating activity
CC of cytokinin oxidases/dehydrogenases (PubMed:25535363). Part of the ER
CC quality control system, which determines the fate of aberrant proteins
CC in the secretory pathway (PubMed:25535363).
CC {ECO:0000269|PubMed:25535363}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:25535363}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8LES0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8LES0-2; Sequence=VSP_042459;
CC -!- TISSUE SPECIFICITY: Expressed in roots, cotyledons, leaves, stems,
CC flowers and siliques. {ECO:0000269|PubMed:25535363}.
CC -!- SIMILARITY: Belongs to the nucleotide-sugar transporter family. CMP-
CC Sialate:CMP antiporter (TC 2.A.7.12) subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA97312.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB019236; BAA97312.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED97981.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97982.1; -; Genomic_DNA.
DR EMBL; AY072020; AAL57713.1; -; mRNA.
DR EMBL; AK226979; BAE99046.1; -; mRNA.
DR EMBL; BT029465; ABK59694.1; -; mRNA.
DR EMBL; BT030009; ABN04747.1; -; mRNA.
DR EMBL; AY085268; AAM62500.1; -; mRNA.
DR RefSeq; NP_569004.1; NM_125899.4. [Q8LES0-1]
DR RefSeq; NP_974995.1; NM_203266.2. [Q8LES0-2]
DR AlphaFoldDB; Q8LES0; -.
DR SMR; Q8LES0; -.
DR STRING; 3702.AT5G65000.1; -.
DR TCDB; 2.A.7.12.15; the drug/metabolite transporter (dmt) superfamily.
DR PaxDb; Q8LES0; -.
DR PRIDE; Q8LES0; -.
DR ProteomicsDB; 222720; -. [Q8LES0-1]
DR EnsemblPlants; AT5G65000.1; AT5G65000.1; AT5G65000. [Q8LES0-1]
DR EnsemblPlants; AT5G65000.2; AT5G65000.2; AT5G65000. [Q8LES0-2]
DR GeneID; 836624; -.
DR Gramene; AT5G65000.1; AT5G65000.1; AT5G65000. [Q8LES0-1]
DR Gramene; AT5G65000.2; AT5G65000.2; AT5G65000. [Q8LES0-2]
DR KEGG; ath:AT5G65000; -.
DR Araport; AT5G65000; -.
DR TAIR; locus:2177674; AT5G65000.
DR eggNOG; KOG2234; Eukaryota.
DR HOGENOM; CLU_046494_0_0_1; -.
DR InParanoid; Q8LES0; -.
DR OMA; CYYFLNP; -.
DR OrthoDB; 703674at2759; -.
DR PhylomeDB; Q8LES0; -.
DR PRO; PR:Q8LES0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8LES0; baseline and differential.
DR Genevisible; Q8LES0; AT.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:TAIR.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030173; C:integral component of Golgi membrane; IBA:GO_Central.
DR GO; GO:0015136; F:sialic acid transmembrane transporter activity; ISS:UniProtKB.
DR GO; GO:0005463; F:UDP-N-acetylgalactosamine transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:1903857; P:negative regulation of cytokinin dehydrogenase activity; IMP:TAIR.
DR GO; GO:1903070; P:negative regulation of ER-associated ubiquitin-dependent protein catabolic process; IMP:TAIR.
DR GO; GO:0010584; P:pollen exine formation; IMP:TAIR.
DR GO; GO:1902183; P:regulation of shoot apical meristem development; IMP:TAIR.
DR GO; GO:0015739; P:sialic acid transport; ISS:UniProtKB.
DR GO; GO:0015789; P:UDP-N-acetylgalactosamine transmembrane transport; IDA:TAIR.
DR GO; GO:1990569; P:UDP-N-acetylglucosamine transmembrane transport; IDA:TAIR.
DR InterPro; IPR007271; Nuc_sug_transpt.
DR PANTHER; PTHR10231; PTHR10231; 1.
DR Pfam; PF04142; Nuc_sug_transp; 1.
DR PIRSF; PIRSF005799; UDP-gal_transpt; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Endoplasmic reticulum; Membrane; Reference proteome;
KW Sugar transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..325
FT /note="UDP-N-acetylglucosamine transporter ROCK1"
FT /id="PRO_0000416028"
FT TOPO_DOM 1..13
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 35..42
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 43..63
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 64..109
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 110..130
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 131..135
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 157..171
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 193..203
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 204..224
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 225..241
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 242..262
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 263..270
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 271..291
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 292..325
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT VAR_SEQ 248..325
FT /note="PVISNALGGILVGLVTSHAGGVRKGFVIVSALLVTALLQFAFEGKPPSSYCL
FT VALPLVMSSISMYQKYPYIDKKKKKV -> INYLFFLSTKQFF (in isoform 2)"
FT /evidence="ECO:0000303|Ref.5"
FT /id="VSP_042459"
FT CONFLICT 25
FT /note="T -> R (in Ref. 3; AAL57713)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 325 AA; 34836 MW; CC84D83A4BCD34BC CRC64;
MATANGAKSP SSMGPKVLFY SILLTLQYGA QPLISKRCIR KDVIVTSSVL TCEIVKVICA
LILMARNGSL KGLAKEWTLM GSLTASGLPA AIYALQNSLL QISYRSLDSL TFSILNQTKI
FFTAFFTFII LRQKQSILQI GALCLLIMAA VLLSVGEGSN KDSSGINADQ KLFYGIIPVL
AASVLSGLAS SLCQWASQVK KHSSYLMTVE MSIVGSLCLL VSTLKSPDGE AIKKYGFFHG
WTALTLVPVI SNALGGILVG LVTSHAGGVR KGFVIVSALL VTALLQFAFE GKPPSSYCLV
ALPLVMSSIS MYQKYPYIDK KKKKV
