ROCO7_DICDI
ID ROCO7_DICDI Reviewed; 2615 AA.
AC Q55GE6; Q6XHA9;
DT 20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Probable serine/threonine-protein kinase roco7;
DE EC=2.7.11.1;
DE AltName: Full=Ras of complex proteins and C-terminal of roc 7;
GN Name=roco7; ORFNames=DDB_G0267472;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=14654223; DOI=10.1016/j.bbamcr.2003.08.008;
RA Bosgraaf L., van Haastert P.J.M.;
RT "Roc, a Ras/GTPase domain in complex proteins.";
RL Biochim. Biophys. Acta 1643:5-10(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1;
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. TKL Ser/Thr
CC protein kinase family. ROCO subfamily. {ECO:0000305}.
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DR EMBL; AY232269; AAO83652.1; -; Genomic_DNA.
DR EMBL; AAFI02000003; EAL73189.1; -; Genomic_DNA.
DR RefSeq; XP_647238.1; XM_642146.1.
DR AlphaFoldDB; Q55GE6; -.
DR SMR; Q55GE6; -.
DR STRING; 44689.DDB0191295; -.
DR PaxDb; Q55GE6; -.
DR EnsemblProtists; EAL73189; EAL73189; DDB_G0267472.
DR GeneID; 8616043; -.
DR KEGG; ddi:DDB_G0267472; -.
DR dictyBase; DDB_G0267472; roco7.
DR eggNOG; KOG0192; Eukaryota.
DR HOGENOM; CLU_227807_0_0_1; -.
DR InParanoid; Q55GE6; -.
DR OMA; WNWRWLA; -.
DR PRO; PR:Q55GE6; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IDA:dictyBase.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004672; F:protein kinase activity; IBA:GO_Central.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR Gene3D; 1.10.506.10; -; 1.
DR Gene3D; 2.130.10.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR032171; COR.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR InterPro; IPR008936; Rho_GTPase_activation_prot.
DR InterPro; IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR Pfam; PF16095; COR; 1.
DR Pfam; PF07714; PK_Tyr_Ser-Thr; 1.
DR PRINTS; PR00109; TYRKINASE.
DR SMART; SM00220; S_TKc; 1.
DR SMART; SM00320; WD40; 2.
DR SUPFAM; SSF50998; SSF50998; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
PE 3: Inferred from homology;
KW ATP-binding; Kinase; Nucleotide-binding; Reference proteome; Repeat;
KW Serine/threonine-protein kinase; Transferase; WD repeat.
FT CHAIN 1..2615
FT /note="Probable serine/threonine-protein kinase roco7"
FT /id="PRO_0000358893"
FT DOMAIN 1775..2042
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REPEAT 2491..2527
FT /note="WD 1"
FT REPEAT 2533..2574
FT /note="WD 2"
FT REGION 1..35
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 275..297
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 533..623
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 946..998
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2061..2158
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2176..2209
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 1899
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10027"
FT BINDING 1781..1789
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 1802
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT CONFLICT 16
FT /note="P -> T (in Ref. 1; AAO83652)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 2615 AA; 290512 MW; 19F1B3AEC13F9F46 CRC64;
MDGYSSLSSS GNSIAPPRPT NNSVGGGSNS NNYNQREITT PLRGMVPNLG DGSSSQTLLF
KNILLLQDVE FFCSILDNFC RSSSTQKITD LINSLIVILD DSFLELLRLF IKQTIDQAKQ
CSTDESNPLN YQSFYNLINR MAAIYLKSQL GGLMEKTMIS QVLNFTPMIQ EQIVILANKE
ANKNNNNVLS ANSTPNVTAL KTSPAIGSPR IIMGSNYNNS SNNNNIDSID LNNNSPNMLK
NNSPSTFING SVPQINGGGG SYNLTPISSP TIGSSTPTII TSSSSTTTPT NNNNSNNNTP
IISQTIIQNI NVEHMKDQIS QFSISIINTI FRSIEKFPPT VFQLFEMLYL DIHQVFSPDF
CFELMKKIFF TKLICPILAN LKSPPNMANG KFFTKTYIEI SKVVLDIAFD RDQSRRGSVT
TQEQIMHFIK GMIFRNTHSI SMTAALNPTL NNQVIPPSIP LPSKEEACNN FINNIRSESS
ELEESFKRAK DLNTCISFAR ADRQIYLFSI FKEMLINFKI SFNLLNNNQN NNQNINQNNN
NNNNQNCNSN TSSPIISSRS GFYTLRNSST NSSPTNPPLT ARGNGSISIG NNNNNNNNNN
NNNNNNNNNN NNNNSGGGGG GLNNSSNLLI NPWVSSYQNS NISNSDQVQS TRVVQSFSKK
WTSDELRPTY LGKSPKKRSW VLDKKNIKTQ IINLHIFLDN YSVCRIVTVP MNITFQALAN
RIHTDSEFSD IQLNKEEYEM VIRYPEESIT VLNDEGNQEV ICEPELPLWM FDIDTESIII
FRPIKRRSPS SSFNIFLKFI FPSNSSFNSS SSSLPSTPHQ KINPMILYIN PQSTPQSIID
KFSKLIQPSL LDTAHLGFYL RDIDDESSSP PSIKIPNNNS FAHNKISTMD IIECGPRLSY
EFSITVNGTI QNILIDFDNQ IDNVANIFYN IYQHLIELSP RSLSSSSSSS SDINNNNNNN
TNNNIELTSS GRILTSNGSK HNSNNSNNSS GSSNISEQNL KRSSGIIDSI INGHSNNNSN
NYALALLSNS NFLPMFLPGQ SKLQDYCFNV GDELILTEKT FVQLVEVLAT PRRPPIALSS
SLSQSNAFNS NVYNSNAYNS NVYNSNPYLS NYNGLSSQSL NSMNVDGAGG VGGFNGEQNQ
QQNKVIARVR VIWAGSNSSV DRLQSFINTS QNVNTTYSGS TMALNDVLSN PNQLLSTPLS
RPLLSTVIAQ DNTYICTSKN DYCMNNQVKL CIVGEESQEK LSFYNSLRKN YSQNGLLSSG
GGRGGGFFGS NFIPSSTSNV NEVSSIDGVL HTSELVVGSA EIDQITLKTF YISGNEQYQV
VHPLFISPQS LFIITYNPMN INSTMINYWL EIIQTKAQGS TVYLVGLSNT SIDEKKFVSF
KSEYHRLFRF NNINSYSNIS LKNSKQIKQL VQRLQNNAMM KQYHYKIPLS YSILKSQCQE
SVKEAYARNK MPLSSIPLIK NIARIFSIEP RDSEAAIKYL YEIGEVLYYR YEANDQLLNE
LVFLDSMWMS KLISAVLALK TQNGMTVVDQ ITQAWASLFP HCKTSSLLFL LEKFELVYIS
AEDSSVIIPQ LFGGERPMVM RDLWSPTAHA NNEYLRIYEF QFLPKGFFSR LSVRVLQHYD
PLCIWQNGMV LQPAGQLWGG AAKSFDSQCL IEYDSVNFIL KVSIRDDNKQ QQLLKSIVDL
VSSFILWYFP GRLSNVRVAC THCTNQHIEN PTMYTLDYLE NQASLGQTNV ICKAQLGGII
HETLSPRTTK IDIYSLAFEV TFNSNKFSVI PYETLKFGPQ LGSGSYANVY RGIWNGSEVA
IKVLNFDDGH ANTTEKYREF RNEAHITGEL RHANTVSLMG VSLSPFCLVT ELLQYGDLAK
FIRNTAETFS WGTVLKLAID VAKGMNFLHS CKPMIVHRDL KSANILLGGS SMDNLVAKVG
DFGLSIKPIG KEVKGRKVWN WRWLAPECMG DGQYTEKIDI YSYAIVLWEI ITRDLPFEEY
VDQLKWNSII EDKIMKGLRP TIPNECPPDM KQLITDCWSG DPKKRPSFNS ILERLSTMQK
TFNLNERLEF CKQLPPINED QINQQQQQPP PQSQAAQQQQ PSTSTPPLSQ HQQKLSISNL
QLNNLLNNHN SNGSNSSIQS SLSHNNNNLN NNINNNNNNN NNNNNNSSGG SSGVSHSGSS
GNNFVIPIIT AIANGGIGSG GNQHQQNGSS TPHSNSQSNS GSSSVYESGD GSLSSAGSFK
ILRYEMVLPV AFTSTIHSLY PVQNNKNEVF VWCGMGDGSV CVINSATRQI VSTSRIADSS
RILGFSLIRK CTPSSSSVTL TSRSSLNLSS LASGSTPSPY SSGGSGGSLG SSYQPICPTI
EEDSHIWAFY NEGILCFEAK SFKLLKTIKT NFITSLVDEG ESVWSNCKEK TSCIKVISKS
KLKTKKLMNV KTLDAQITTI LIHHSSVGAV GASRVWLGTD RGMIFILEYP SMAPIAHHES
HGGALIHTIK RMDRFVITCS ERVICIFDES GIIKKRLDGL ASRVLSLLIL DTYIIGACYD
STILVWDSKQ NFRMVQSLKK KHTDAISSLA FALSPQGKPQ LWVGGWDKKI TTYSFFEDLE
SSLSIALQTP PPSSYPTLAT PRLGLVNVSK SKLFG
