ROF_ECOLI
ID ROF_ECOLI Reviewed; 84 AA.
AC P0AFW8; P52098; P75670; Q9WVU2;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Protein rof;
GN Name=rof; Synonyms=yaeO; OrderedLocusNames=b0189, JW0184;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RC STRAIN=K12;
RX PubMed=9723924; DOI=10.1046/j.1365-2958.1998.00981.x;
RA Pichoff S., Alibaud L., Guedant A., Castanie M.-P., Bouche J.-P.;
RT "An Escherichia coli gene (yaeO) suppresses temperature-sensitive mutations
RT in essential genes by modulating Rho-dependent transcription termination.";
RL Mol. Microbiol. 29:859-869(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RA Yamamoto Y.;
RL Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RA Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T.,
RA Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S.,
RA Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.;
RT "Systematic sequencing of the Escherichia coli genome: analysis of the 4.0
RT - 6.0 min (189,987 - 281,416bp) region.";
RL Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION TO
RP 72-73.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- FUNCTION: Suppresses temperature-sensitive mutations in essential genes
CC by modulating rho-dependent transcription termination.
CC -!- INTERACTION:
CC P0AFW8; P0AG30: rho; NbExp=2; IntAct=EBI-1114609, EBI-545468;
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA90752.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Z50870; CAA90752.1; ALT_INIT; Genomic_DNA.
DR EMBL; D49445; BAA08429.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73300.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA77864.2; -; Genomic_DNA.
DR RefSeq; NP_414731.2; NC_000913.3.
DR PDB; 1SG5; NMR; -; A=1-84.
DR PDBsum; 1SG5; -.
DR AlphaFoldDB; P0AFW8; -.
DR SMR; P0AFW8; -.
DR BioGRID; 4260946; 399.
DR BioGRID; 849291; 1.
DR IntAct; P0AFW8; 11.
DR MINT; P0AFW8; -.
DR STRING; 511145.b0189; -.
DR jPOST; P0AFW8; -.
DR PaxDb; P0AFW8; -.
DR PRIDE; P0AFW8; -.
DR EnsemblBacteria; AAC73300; AAC73300; b0189.
DR EnsemblBacteria; BAA77864; BAA77864; BAA77864.
DR GeneID; 944891; -.
DR KEGG; ecj:JW0184; -.
DR KEGG; eco:b0189; -.
DR PATRIC; fig|511145.12.peg.196; -.
DR EchoBASE; EB3012; -.
DR eggNOG; COG4568; Bacteria.
DR HOGENOM; CLU_176324_0_1_6; -.
DR OMA; TEYQPIN; -.
DR PhylomeDB; P0AFW8; -.
DR BioCyc; EcoCyc:G6097-MON; -.
DR EvolutionaryTrace; P0AFW8; -.
DR PRO; PR:P0AFW8; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0031554; P:regulation of DNA-templated transcription, termination; IMP:EcoCyc.
DR GO; GO:0031564; P:transcription antitermination; IMP:EcoCyc.
DR Gene3D; 2.30.30.400; -; 1.
DR InterPro; IPR009778; ROF.
DR InterPro; IPR038626; Rof-like_sf.
DR InterPro; IPR023534; Rof/RNase_P-like.
DR Pfam; PF07073; ROF; 1.
DR SUPFAM; SSF101744; SSF101744; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome.
FT CHAIN 1..84
FT /note="Protein rof"
FT /id="PRO_0000097397"
FT HELIX 13..20
FT /evidence="ECO:0007829|PDB:1SG5"
FT TURN 21..24
FT /evidence="ECO:0007829|PDB:1SG5"
FT STRAND 26..29
FT /evidence="ECO:0007829|PDB:1SG5"
FT TURN 31..33
FT /evidence="ECO:0007829|PDB:1SG5"
FT STRAND 36..40
FT /evidence="ECO:0007829|PDB:1SG5"
FT STRAND 42..44
FT /evidence="ECO:0007829|PDB:1SG5"
FT STRAND 47..49
FT /evidence="ECO:0007829|PDB:1SG5"
FT STRAND 51..56
FT /evidence="ECO:0007829|PDB:1SG5"
FT STRAND 59..64
FT /evidence="ECO:0007829|PDB:1SG5"
FT STRAND 69..73
FT /evidence="ECO:0007829|PDB:1SG5"
FT TURN 74..76
FT /evidence="ECO:0007829|PDB:1SG5"
FT STRAND 77..79
FT /evidence="ECO:0007829|PDB:1SG5"
SQ SEQUENCE 84 AA; 9480 MW; 1BD1E254B0F0A82E CRC64;
MNDTYQPINC DDYDNLELAC QHHLMLTLEL KDGEKLQAKA SDLVSRKNVE YLVVEAAGET
RELRLDKITS FSHPEIGTVV VSES
