ROGDI_DROME
ID ROGDI_DROME Reviewed; 268 AA.
AC Q9VVE2; Q32KF2; Q8IQP4; Q95T16;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Protein rogdi;
GN Name=rogdi; ORFNames=CG7725;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, AND MUTAGENESIS OF 16-GLN--ALA-83.
RX PubMed=28900300; DOI=10.1038/s41598-017-11941-3;
RA Kim M., Jang D., Yoo E., Oh Y., Sonn J.Y., Lee J., Ki Y., Son H.J.,
RA Hwang O., Lee C., Lim C., Choe J.;
RT "Rogdi Defines GABAergic Control of a Wake-promoting Dopaminergic Pathway
RT to Sustain Sleep in Drosophila.";
RL Sci. Rep. 7:11368-11368(2017).
CC -!- FUNCTION: Plays a role in promoting sleep by enhancing metabotropic
CC transmission of the inhibitory neurotransmitter gamma-aminobutyric acid
CC (GABA) in GABAergic neurons upstream of a wake-inducing dopaminergic
CC pathway. {ECO:0000269|PubMed:28900300}.
CC -!- SUBCELLULAR LOCATION: Nucleus envelope {ECO:0000250|UniProtKB:Q9GZN7}.
CC Presynapse {ECO:0000250|UniProtKB:Q4V7D2}. Cell projection, axon
CC {ECO:0000250|UniProtKB:Q4V7D2}. Perikaryon
CC {ECO:0000250|UniProtKB:Q4V7D2}. Cell projection, dendrite
CC {ECO:0000250|UniProtKB:Q4V7D2}. Cytoplasmic vesicle, secretory vesicle,
CC synaptic vesicle {ECO:0000250|UniProtKB:Q4V7D2}. Note=Detected
CC primarily at presynaptic sites on axons, and to a lesser degree in soma
CC and dendrites. Not detected at post-synaptic sites.
CC {ECO:0000250|UniProtKB:Q4V7D2}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9VVE2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9VVE2-2; Sequence=VSP_030602;
CC -!- TISSUE SPECIFICITY: Expressed in both anterior and posterior regions of
CC the brain including in GABAergic neurons.
CC {ECO:0000269|PubMed:28900300}.
CC -!- SIMILARITY: Belongs to the rogdi family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABB36431.1; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE014296; AAF49370.2; -; Genomic_DNA.
DR EMBL; AE014296; AAN11734.1; -; Genomic_DNA.
DR EMBL; AY060379; AAL25418.1; -; mRNA.
DR EMBL; BT023927; ABB36431.1; ALT_SEQ; mRNA.
DR RefSeq; NP_648956.1; NM_140699.3. [Q9VVE2-1]
DR RefSeq; NP_730244.1; NM_168717.2. [Q9VVE2-2]
DR AlphaFoldDB; Q9VVE2; -.
DR SMR; Q9VVE2; -.
DR BioGRID; 65209; 13.
DR IntAct; Q9VVE2; 3.
DR STRING; 7227.FBpp0075042; -.
DR PaxDb; Q9VVE2; -.
DR PRIDE; Q9VVE2; -.
DR DNASU; 39917; -.
DR EnsemblMetazoa; FBtr0075282; FBpp0075042; FBgn0036697. [Q9VVE2-2]
DR EnsemblMetazoa; FBtr0075283; FBpp0075043; FBgn0036697. [Q9VVE2-1]
DR GeneID; 39917; -.
DR KEGG; dme:Dmel_CG7725; -.
DR UCSC; CG7725-RA; d. melanogaster. [Q9VVE2-1]
DR CTD; 79641; -.
DR FlyBase; FBgn0036697; rogdi.
DR VEuPathDB; VectorBase:FBgn0036697; -.
DR eggNOG; KOG3992; Eukaryota.
DR GeneTree; ENSGT00390000007164; -.
DR HOGENOM; CLU_062094_0_0_1; -.
DR InParanoid; Q9VVE2; -.
DR OMA; NILMECA; -.
DR PhylomeDB; Q9VVE2; -.
DR SignaLink; Q9VVE2; -.
DR BioGRID-ORCS; 39917; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 39917; -.
DR PRO; PR:Q9VVE2; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0036697; Expressed in embryonic/larval hemocyte (Drosophila) and 25 other tissues.
DR Genevisible; Q9VVE2; DM.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0030424; C:axon; IEA:UniProtKB-SubCell.
DR GO; GO:0030425; C:dendrite; IEA:UniProtKB-SubCell.
DR GO; GO:0005635; C:nuclear envelope; IEA:UniProtKB-SubCell.
DR GO; GO:0043204; C:perikaryon; IEA:UniProtKB-SubCell.
DR GO; GO:0043291; C:RAVE complex; IBA:GO_Central.
DR GO; GO:0008021; C:synaptic vesicle; IEA:UniProtKB-SubCell.
DR GO; GO:0048149; P:behavioral response to ethanol; IMP:FlyBase.
DR GO; GO:0061534; P:gamma-aminobutyric acid secretion, neurotransmission; IMP:UniProtKB.
DR GO; GO:0030431; P:sleep; IMP:UniProtKB.
DR InterPro; IPR028241; RAVE2/Rogdi.
DR PANTHER; PTHR13618; PTHR13618; 1.
DR Pfam; PF10259; Rogdi_lz; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell projection; Cytoplasmic vesicle; Nucleus;
KW Reference proteome; Synapse.
FT CHAIN 1..268
FT /note="Protein rogdi"
FT /id="PRO_0000315670"
FT VAR_SEQ 1
FT /note="M -> MAQSTTNNWSFQPLHRQRSTSGYPQHVLNTVPETAVLTTPPPTRKPL
FT TIQIAGVAAAESGHKRRFLAARKKPKSAM (in isoform 2)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_030602"
FT MUTAGEN 16..83
FT /note="Missing: In rogdi(del); greatly reduced protein
FT half-life; localization to distinct cytoplasmic inclusions;
FT insomnia-like behaviors with sleep fragmentation and delay
FT in sleep initiation in males and virgin females but not in
FT mated females."
FT /evidence="ECO:0000269|PubMed:28900300"
FT CONFLICT 207
FT /note="N -> D (in Ref. 4; ABB36431)"
FT /evidence="ECO:0000305"
FT CONFLICT 234
FT /note="M -> V (in Ref. 4; ABB36431)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 268 AA; 30495 MW; 003CF91044D42658 CRC64;
MKMLADTERE EALNLQIEFE WVLRQEVHAI LKQLRSILVE CAHRFPVPLY ENEGKKTEKF
ILTVSPDQLK AVLTLTGDAI TQADISFKLC KAPSQTQRTS ITHDSPWKLQ QVQDAANHLQ
TAINHIDDVD DSYHFKTSDE VLHVIGNILD ALQRGRNSLL VPKKKPIDEL IKGRNMKSLV
PNLPEDLAVS FYLQSHKLII AVYQLLNNQG TMRFDSRQAE ASVQWLNDVL LLLMNGQKLC
QQLKDKISVF SVYKDFTVGS RSPSALSY
