ROGF4_ARATH
ID ROGF4_ARATH Reviewed; 463 AA.
AC Q0WNP7; O80830;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 1.
DT 25-MAY-2022, entry version 96.
DE RecName: Full=Rop guanine nucleotide exchange factor 4;
DE Short=AtRopGEF4;
DE AltName: Full=Rho of plants guanine nucleotide exchange factor 4;
GN Name=ROPGEF4; OrderedLocusNames=At2g45890; ORFNames=F4I18.13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY.
RX PubMed=15980860; DOI=10.1038/nature03883;
RA Berken A., Thomas C., Wittinghofer A.;
RT "A new family of RhoGEFs activates the Rop molecular switch in plants.";
RL Nature 436:1176-1180(2005).
RN [5]
RP FUNCTION, INTERACTION WITH ARAC10/ROP11, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=22500990; DOI=10.1016/j.febslet.2012.03.040;
RA Li Z., Liu D.;
RT "ROPGEF1 and ROPGEF4 are functional regulators of ROP11 GTPase in ABA-
RT mediated stomatal closure in Arabidopsis.";
RL FEBS Lett. 586:1253-1258(2012).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=22984069; DOI=10.1126/science.1222597;
RA Oda Y., Fukuda H.;
RT "Initiation of cell wall pattern by a Rho- and microtubule-driven symmetry
RT breaking.";
RL Science 337:1333-1336(2012).
CC -!- FUNCTION: Guanine-nucleotide exchange factor (GEF) that acts as an
CC activator of Rop (Rho of plants) GTPases by promoting the exchange of
CC GDP for GTP. In association with ROPGEF1, acts as specific regulator of
CC ARAC10/ROP11 function in ABA-mediated stomatal closure.
CC {ECO:0000269|PubMed:22500990, ECO:0000269|PubMed:22984069}.
CC -!- SUBUNIT: Interacts with ARAC10/ROP11. {ECO:0000269|PubMed:22500990}.
CC -!- INTERACTION:
CC Q0WNP7; O82481: ARAC10; NbExp=5; IntAct=EBI-7819454, EBI-1548072;
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Cell membrane. Note=Interacts with
CC ARAC10/ROP11 on plasma membrane in guard cells.
CC -!- TISSUE SPECIFICITY: Expressed in root vascular tissue and trichoblast
CC cell files. Expressed in root metaxylem cell files. Expressed in guard
CC cells of cotyledons, rosette leaves, sepals, petal, stigmas and
CC siliques. Expressed in root metaxylem cell files.
CC {ECO:0000269|PubMed:22500990, ECO:0000269|PubMed:22984069}.
CC -!- DOMAIN: The PRONE (plant-specific Rop nucleotide exchanger) domain is
CC responsible for the GEF activity. {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but mutant plant roots have reduced density of secondary
CC wall pits in metaxylem vessels. {ECO:0000269|PubMed:22500990,
CC ECO:0000269|PubMed:22984069}.
CC -!- MISCELLANEOUS: Plants overexpressing ROPGEF1 or ROPGEF4 are relatively
CC insensitive to ABA-induced stomatal closure and become severely wilted
CC during drought stress. A similar phenotype is observed in plants
CC expressing a constitutively active ARAC10/ROP11 (PubMed:22500990).
CC {ECO:0000305|PubMed:22500990}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC28535.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC004665; AAC28535.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC10614.1; -; Genomic_DNA.
DR EMBL; AK229390; BAF01252.1; -; mRNA.
DR PIR; T02458; T02458.
DR RefSeq; NP_182113.2; NM_130152.3.
DR AlphaFoldDB; Q0WNP7; -.
DR SMR; Q0WNP7; -.
DR BioGRID; 4533; 4.
DR DIP; DIP-59395N; -.
DR IntAct; Q0WNP7; 5.
DR MINT; Q0WNP7; -.
DR STRING; 3702.AT2G45890.1; -.
DR iPTMnet; Q0WNP7; -.
DR PaxDb; Q0WNP7; -.
DR PRIDE; Q0WNP7; -.
DR ProteomicsDB; 227969; -.
DR EnsemblPlants; AT2G45890.1; AT2G45890.1; AT2G45890.
DR GeneID; 819197; -.
DR Gramene; AT2G45890.1; AT2G45890.1; AT2G45890.
DR KEGG; ath:AT2G45890; -.
DR Araport; AT2G45890; -.
DR TAIR; locus:2050699; AT2G45890.
DR eggNOG; ENOG502QV6R; Eukaryota.
DR HOGENOM; CLU_019073_4_1_1; -.
DR InParanoid; Q0WNP7; -.
DR OMA; DICKIQF; -.
DR OrthoDB; 482824at2759; -.
DR PhylomeDB; Q0WNP7; -.
DR PRO; PR:Q0WNP7; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q0WNP7; baseline and differential.
DR Genevisible; Q0WNP7; AT.
DR GO; GO:0016324; C:apical plasma membrane; IDA:TAIR.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0009531; C:secondary cell wall; IDA:TAIR.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISS:TAIR.
DR GO; GO:0050832; P:defense response to fungus; IMP:TAIR.
DR GO; GO:0009664; P:plant-type cell wall organization; IMP:TAIR.
DR GO; GO:0048767; P:root hair elongation; IMP:TAIR.
DR InterPro; IPR005512; PRONE_dom.
DR InterPro; IPR038937; RopGEF.
DR PANTHER; PTHR33101; PTHR33101; 1.
DR Pfam; PF03759; PRONE; 1.
DR PROSITE; PS51334; PRONE; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Cytoplasm; Guanine-nucleotide releasing factor; Membrane;
KW Reference proteome; Stress response.
FT CHAIN 1..463
FT /note="Rop guanine nucleotide exchange factor 4"
FT /id="PRO_0000423890"
FT DOMAIN 84..463
FT /note="PRONE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00663"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 55..87
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 463 AA; 52035 MW; 41C2DA9959AA74C1 CRC64;
MESSSNSDQN EGTPTSSVSS PYRRTYSDIS GLSHRFDVQS FYNRPSNTNA VVLSGHEEDV
SEDAEEPKDD VVNDVHGDGD EEDSDIDSAE DAELEMMRER FAKLLLGEDM SGSGKGVCTA
VTVSNSITNL YATVFGQSLR LQPLSTEKKD LWKREMNCFM SICDYIVEVI PRSLGTNVEI
TETKLRSDIL MSLPALRKLD NMLMEILDSF TENEFWYVER GSSSMNSGGG GRDSGTFRKV
VVQRKDEKWW LPVPCVPAEG LSEEERKHLR HKRDCASQIH KAALAINDST LNDMDIPDSY
LTTLPKSGKA SVGDVIYKQL CTAEKFYPDQ LLDILKITSE HEALELADKV EASLVTWRRK
TGGLTHSKSS WDMMKDISGD ADRGNDKNHI LAARARSLLF CLKQRYPELS QTSLDICKIQ
FNRDVGKAVL ESYSRVLEGL AYNVVSWIDD VLYVDRTVRN RDD
