ROGF9_ARATH
ID ROGF9_ARATH Reviewed; 517 AA.
AC Q9SVQ3; A0MF70;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Rop guanine nucleotide exchange factor 9 {ECO:0000303|PubMed:15980860};
DE Short=AtRopGEF9 {ECO:0000303|PubMed:15980860};
DE AltName: Full=Rho of plants guanine nucleotide exchange factor 9 {ECO:0000303|PubMed:15980860};
GN Name=ROPGEF9 {ECO:0000303|PubMed:15980860};
GN OrderedLocusNames=At4g13240 {ECO:0000312|Araport:AT4G13240};
GN ORFNames=F17N18.130 {ECO:0000312|EMBL:CAB41934.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [4]
RP GENE FAMILY.
RX PubMed=15980860; DOI=10.1038/nature03883;
RA Berken A., Thomas C., Wittinghofer A.;
RT "A new family of RhoGEFs activates the Rop molecular switch in plants.";
RL Nature 436:1176-1180(2005).
RN [5]
RP INTERACTION WITH ARAC11/ROP1, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=16415208; DOI=10.1105/tpc.105.036434;
RA Gu Y., Li S., Lord E.M., Yang Z.;
RT "Members of a novel class of Arabidopsis Rho guanine nucleotide exchange
RT factors control Rho GTPase-dependent polar growth.";
RL Plant Cell 18:366-381(2006).
RN [6]
RP INTERACTION WITH ARAC10/ROP11, AND TISSUE SPECIFICITY.
RX PubMed=22500990; DOI=10.1016/j.febslet.2012.03.040;
RA Li Z., Liu D.;
RT "ROPGEF1 and ROPGEF4 are functional regulators of ROP11 GTPase in ABA-
RT mediated stomatal closure in Arabidopsis.";
RL FEBS Lett. 586:1253-1258(2012).
RN [7]
RP INTERACTION WITH PRK6.
RX PubMed=26961657; DOI=10.1038/nature17413;
RA Takeuchi H., Higashiyama T.;
RT "Tip-localized receptors control pollen tube growth and LURE sensing in
RT Arabidopsis.";
RL Nature 531:245-248(2016).
CC -!- FUNCTION: Guanine-nucleotide exchange factor (GEF) that acts as an
CC activator of Rop (Rho of plants) GTPases by promoting the exchange of
CC GDP for GTP. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with ARAC11/ROP1 and ARAC10/ROP11. Interacts with
CC PRK6 (PubMed:26961657). {ECO:0000269|PubMed:16415208,
CC ECO:0000269|PubMed:22500990, ECO:0000269|PubMed:26961657}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:16415208}.
CC Note=Localizes to the apical region of the pollen tube plasma membrane.
CC -!- TISSUE SPECIFICITY: Expressed in pollen grains and pollen tubes.
CC {ECO:0000269|PubMed:16415208, ECO:0000269|PubMed:22500990}.
CC -!- DOMAIN: The PRONE (plant-specific Rop nucleotide exchanger) domain is
CC responsible for the GEF activity. {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABK28630.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR EMBL; AL049751; CAB41934.1; -; Genomic_DNA.
DR EMBL; AL161535; CAB78366.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE83250.1; -; Genomic_DNA.
DR EMBL; DQ446830; ABE66063.1; -; mRNA.
DR EMBL; DQ653196; ABK28630.1; ALT_SEQ; mRNA.
DR PIR; T07704; T07704.
DR RefSeq; NP_193060.1; NM_117395.3.
DR AlphaFoldDB; Q9SVQ3; -.
DR SMR; Q9SVQ3; -.
DR BioGRID; 12238; 2.
DR IntAct; Q9SVQ3; 1.
DR MINT; Q9SVQ3; -.
DR STRING; 3702.AT4G13240.1; -.
DR PaxDb; Q9SVQ3; -.
DR PRIDE; Q9SVQ3; -.
DR ProteomicsDB; 228015; -.
DR EnsemblPlants; AT4G13240.1; AT4G13240.1; AT4G13240.
DR GeneID; 826941; -.
DR Gramene; AT4G13240.1; AT4G13240.1; AT4G13240.
DR KEGG; ath:AT4G13240; -.
DR Araport; AT4G13240; -.
DR TAIR; locus:2119320; AT4G13240.
DR eggNOG; ENOG502QSGR; Eukaryota.
DR HOGENOM; CLU_019073_1_0_1; -.
DR InParanoid; Q9SVQ3; -.
DR OMA; NNSIHWQ; -.
DR OrthoDB; 482824at2759; -.
DR PhylomeDB; Q9SVQ3; -.
DR PRO; PR:Q9SVQ3; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9SVQ3; baseline and differential.
DR Genevisible; Q9SVQ3; AT.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISS:TAIR.
DR GO; GO:0080092; P:regulation of pollen tube growth; IGI:TAIR.
DR InterPro; IPR005512; PRONE_dom.
DR InterPro; IPR038937; RopGEF.
DR PANTHER; PTHR33101; PTHR33101; 1.
DR Pfam; PF03759; PRONE; 1.
DR PROSITE; PS51334; PRONE; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Guanine-nucleotide releasing factor; Membrane;
KW Reference proteome.
FT CHAIN 1..517
FT /note="Rop guanine nucleotide exchange factor 9"
FT /id="PRO_0000423894"
FT DOMAIN 65..429
FT /note="PRONE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00663"
FT REGION 16..76
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 428..517
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 16..62
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 432..487
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 517 AA; 58173 MW; 2726C80BBBCF9AD8 CRC64;
MVPSLERGIS ITSSFNLDRM FDSSPGKEQQ QPHLAETTMP ESQTQDSLGG SPVETSRPMT
SRLISRRQDK QQSETEMMKD RFTKLLLGED MSGGGKGVSS ALALSNAITN LAASIFGEQT
KLQPMAPDRR ARWKKEIDWL LSVTDHIVEF VPSQQISKEG VCTEIMVTRQ RGDLLMNIPA
LRKLDAMLID TLDNFRGHNE FWYVSRDSEE GKQARNERTK DKWWLPPVKV PPNGLSESAR
RMLHFQKDSV SQVQKAAMAI NAQVLSEMAI PDSYIESLPK NGRVSLGDSL YKSITEEWFD
PEQFLSTLDL STEHKVLDVK NRIEASIVIW KRKLHLKDNK SSWGSAVSLE KRELFEERAE
TILVLLKQKF PGLPQSSLDI SKIQYNKDVG HAVLESYSRI LESLGYTEMS RIDDVLYADS
LARKQCTGEE TSDGGKIATE TDSASAGSSN YSGEEIEKLE SQNSSKTTLL DFIGWSDNSS
KGQSEKPPKS PRMTPKKLSY LEKLENLNGF RSPKDRH
