ROGFB_ARATH
ID ROGFB_ARATH Reviewed; 543 AA.
AC Q9M811; B3LF51; F4ICV0;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 17-FEB-2016, sequence version 2.
DT 25-MAY-2022, entry version 108.
DE RecName: Full=Rop guanine nucleotide exchange factor 11;
DE Short=AtRopGEF11;
DE AltName: Full=Phytochrome interacting RopGEF 1;
DE AltName: Full=Rho of plants guanine nucleotide exchange factor 11;
GN Name=ROPGEF11; Synonyms=PIRF1; OrderedLocusNames=At1g52240;
GN ORFNames=F9I5.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 5-414.
RC STRAIN=cv. Columbia;
RA De Los Reyes C., Quan R., Chen H., Bautista V.R., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY.
RX PubMed=15980860; DOI=10.1038/nature03883;
RA Berken A., Thomas C., Wittinghofer A.;
RT "A new family of RhoGEFs activates the Rop molecular switch in plants.";
RL Nature 436:1176-1180(2005).
RN [5]
RP FUNCTION, INTERACTION WITH ARAC4/ROP2; ARAC3/ROP6 ARAC9/ROP8; PHYA AND
RP PHYB, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=20551316; DOI=10.1074/jbc.m110.133710;
RA Shin D.H., Cho M.H., Kim T.L., Yoo J., Kim J.I., Han Y.J., Song P.S.,
RA Jeon J.S., Bhoo S.H., Hahn T.R.;
RT "A small GTPase activator protein interacts with cytoplasmic phytochromes
RT in regulating root development.";
RL J. Biol. Chem. 285:32151-32159(2010).
CC -!- FUNCTION: Guanine-nucleotide exchange factor (GEF) that acts as an
CC activator of Rop (Rho of plants) GTPases by promoting the exchange of
CC GDP for GTP. Functions as a light-signaling switch that functions in
CC root growth and development through the activation of Rop in a
CC phytochrome-dependent manner. May act as a negative regulator of
CC phytochrome-mediated primary root development.
CC {ECO:0000269|PubMed:20551316}.
CC -!- SUBUNIT: Interacts with ARAC4/ROP2, ARAC3/ROP, ARAC9/ROP8, PHYA and
CC PHYB. {ECO:0000269|PubMed:20551316}.
CC -!- INTERACTION:
CC Q9M811; Q93ZY2: ROPGEF1; NbExp=3; IntAct=EBI-16206717, EBI-4425188;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:20551316}.
CC Note=Interacts with PHYA and PHYB in the cytoplasm in darkness.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9M811-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Highly expressed in elongating regions of roots and
CC pollen grains. Expressed in flowers, and at lower levels in leaves and
CC stems. {ECO:0000269|PubMed:20551316}.
CC -!- DOMAIN: The PRONE (plant-specific Rop nucleotide exchanger) domain is
CC responsible for the GEF activity. {ECO:0000250}.
CC -!- MISCELLANEOUS: Plants overexpressing ROPGEF11 show retarded root
CC elongation and irregular root hair formation.
CC {ECO:0000305|PubMed:20551316}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF29411.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC022354; AAF29411.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002684; AEE32773.2; -; Genomic_DNA.
DR EMBL; BT033039; ACE79741.1; -; mRNA.
DR PIR; D96562; D96562.
DR RefSeq; NP_175634.6; NM_104103.6. [Q9M811-1]
DR AlphaFoldDB; Q9M811; -.
DR SMR; Q9M811; -.
DR BioGRID; 26879; 5.
DR DIP; DIP-61929N; -.
DR IntAct; Q9M811; 1.
DR STRING; 3702.AT1G52240.1; -.
DR PaxDb; Q9M811; -.
DR PRIDE; Q9M811; -.
DR ProteomicsDB; 228190; -. [Q9M811-1]
DR EnsemblPlants; AT1G52240.1; AT1G52240.1; AT1G52240. [Q9M811-1]
DR GeneID; 841654; -.
DR Gramene; AT1G52240.1; AT1G52240.1; AT1G52240. [Q9M811-1]
DR KEGG; ath:AT1G52240; -.
DR Araport; AT1G52240; -.
DR TAIR; locus:2018169; AT1G52240.
DR eggNOG; KOG3430; Eukaryota.
DR HOGENOM; CLU_019073_1_0_1; -.
DR OMA; WVKEIGW; -.
DR PRO; PR:Q9M811; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9M811; baseline and differential.
DR Genevisible; Q9M811; AT.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISS:TAIR.
DR GO; GO:0048364; P:root development; IMP:TAIR.
DR GO; GO:0080147; P:root hair cell development; IMP:TAIR.
DR InterPro; IPR005512; PRONE_dom.
DR InterPro; IPR038937; RopGEF.
DR PANTHER; PTHR33101; PTHR33101; 1.
DR Pfam; PF03759; PRONE; 1.
DR PROSITE; PS51334; PRONE; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Guanine-nucleotide releasing factor;
KW Reference proteome.
FT CHAIN 1..543
FT /note="Rop guanine nucleotide exchange factor 11"
FT /id="PRO_0000423896"
FT DOMAIN 95..456
FT /note="PRONE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00663"
FT REGION 61..89
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 543 AA; 61655 MW; 0E0DF925F43F4B42 CRC64;
MVKAMEQEQE TYKSRLFHFK NMNENSASRH VKSWSSDCAM RMDGSDNLDD DDNDMMMFRS
QPGKCGSVDR PSLPIGGVTP NRNDKLPRVS SSDSMEALII LQAAMEQMKE KFSKLLLGED
MSGGGKGVSS ALALSNAITN LAASAFGEQR RLEPMAVDRK TRWRREIGWL ISVADYIVEF
APTQQTNKDG TSMEVMSTRQ RTDLLCNIPA LKKLDAMLLD CLDKFKDQDE FYYVKKDSPD
SCETRNDEKW WLPAVKVPPN GLSEISRRFL QSQKECVNQV LKAAMAINAQ VLSEMEIPES
YLESLPKNGR ASLGDVIYRM ITVEMFDADQ FLIEMDLSSE HKILDLKNRI EASIVIWKRK
MVQKDTKSPW GSTVSIEKRE QFEERAETIL LLLKQGFPGI SQSSLDISKI QFNRDVGLAI
LESYSRVLES LAHTVMSRIE DVLYADQLTQ EPTNNAPSKN RYSLKENEKL REERLSFTED
MASGTLSDVM QWGNKNNEMK KESFFGDREK PLLSKVTGIM TNNKKSSYLD NLGAMRSPTA
RYS
