ROGFC_ARATH
ID ROGFC_ARATH Reviewed; 515 AA.
AC Q9CA89;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Rop guanine nucleotide exchange factor 12 {ECO:0000303|PubMed:15980860};
DE Short=AtRopGEF12 {ECO:0000303|PubMed:15980860};
DE AltName: Full=Protein MATERNAL EFFECT EMBRYO ARREST 64;
DE AltName: Full=Rho of plants guanine nucleotide exchange factor 12 {ECO:0000303|PubMed:15980860};
GN Name=ROPGEF12 {ECO:0000303|PubMed:15980860}; Synonyms=MEEA64;
GN OrderedLocusNames=At1g79860 {ECO:0000312|Araport:AT1G79860};
GN ORFNames=F19K16.18 {ECO:0000312|EMBL:AAG52233.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA De Los Reyes C., Quan R., Chen H., Bautista V.R., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=16415208; DOI=10.1105/tpc.105.036434;
RA Gu Y., Li S., Lord E.M., Yang Z.;
RT "Members of a novel class of Arabidopsis Rho guanine nucleotide exchange
RT factors control Rho GTPase-dependent polar growth.";
RL Plant Cell 18:366-381(2006).
RN [5]
RP GENE FAMILY.
RX PubMed=15980860; DOI=10.1038/nature03883;
RA Berken A., Thomas C., Wittinghofer A.;
RT "A new family of RhoGEFs activates the Rop molecular switch in plants.";
RL Nature 436:1176-1180(2005).
RN [6]
RP FUNCTION, ACTIVITY REGULATION, INTERACTION WITH PRK2, MUTAGENESIS OF
RP THR-458; SER-500 AND SER-510, TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RX PubMed=18000057; DOI=10.1073/pnas.0705874104;
RA Zhang Y., McCormick S.;
RT "A distinct mechanism regulating a pollen-specific guanine nucleotide
RT exchange factor for the small GTPase Rop in Arabidopsis thaliana.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:18830-18835(2007).
RN [7]
RP INTERACTION WITH PRK6.
RX PubMed=26961657; DOI=10.1038/nature17413;
RA Takeuchi H., Higashiyama T.;
RT "Tip-localized receptors control pollen tube growth and LURE sensing in
RT Arabidopsis.";
RL Nature 531:245-248(2016).
CC -!- FUNCTION: Guanine-nucleotide exchange factor (GEF) that acts as an
CC activator of Rop (Rho of plants) GTPases by promoting the exchange of
CC GDP for GTP. May be recruited by PRK2 at the plasma membrane to
CC maintain polar Rop activity in the pollen tube and control polarized
CC pollen tube growth. {ECO:0000269|PubMed:18000057}.
CC -!- ACTIVITY REGULATION: Phosphorylation at Ser-510 by PRK2 may release
CC ROPGEF12 auto-inhibition, thereby activating ROPGEF12 and downstream
CC Rop signaling. {ECO:0000305|PubMed:18000057}.
CC -!- SUBUNIT: Interacts (via C-terminus) with PRK2. Interacts with PRK6
CC (PubMed:26961657). {ECO:0000269|PubMed:18000057,
CC ECO:0000269|PubMed:26961657}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Cell membrane
CC {ECO:0000269|PubMed:18000057}. Note=ROPGEF12 colocalizes with PRK2 at
CC the apical plasma membrane of pollen tube.
CC {ECO:0000269|PubMed:18000057}.
CC -!- TISSUE SPECIFICITY: Expressed in pollen grains.
CC {ECO:0000269|PubMed:16415208, ECO:0000269|PubMed:18000057}.
CC -!- DOMAIN: The PRONE (plant-specific Rop nucleotide exchanger) domain is
CC responsible for the GEF activity. {ECO:0000250}.
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DR EMBL; AC011717; AAG52233.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE36314.1; -; Genomic_DNA.
DR EMBL; BT033042; ACE79744.1; -; mRNA.
DR PIR; F96829; F96829.
DR RefSeq; NP_178104.1; NM_106635.4.
DR AlphaFoldDB; Q9CA89; -.
DR SMR; Q9CA89; -.
DR BioGRID; 29543; 1.
DR DIP; DIP-46186N; -.
DR IntAct; Q9CA89; 1.
DR STRING; 3702.AT1G79860.1; -.
DR PaxDb; Q9CA89; -.
DR PRIDE; Q9CA89; -.
DR ProteomicsDB; 228073; -.
DR EnsemblPlants; AT1G79860.1; AT1G79860.1; AT1G79860.
DR GeneID; 844325; -.
DR Gramene; AT1G79860.1; AT1G79860.1; AT1G79860.
DR KEGG; ath:AT1G79860; -.
DR Araport; AT1G79860; -.
DR TAIR; locus:2017844; AT1G79860.
DR eggNOG; ENOG502QSGR; Eukaryota.
DR HOGENOM; CLU_019073_1_0_1; -.
DR InParanoid; Q9CA89; -.
DR OMA; MKRLEPM; -.
DR OrthoDB; 482824at2759; -.
DR PhylomeDB; Q9CA89; -.
DR PRO; PR:Q9CA89; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9CA89; baseline and differential.
DR Genevisible; Q9CA89; AT.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0090406; C:pollen tube; IDA:TAIR.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISS:TAIR.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0009860; P:pollen tube growth; IEP:TAIR.
DR GO; GO:0080092; P:regulation of pollen tube growth; IGI:TAIR.
DR InterPro; IPR005512; PRONE_dom.
DR InterPro; IPR038937; RopGEF.
DR PANTHER; PTHR33101; PTHR33101; 1.
DR Pfam; PF03759; PRONE; 1.
DR PROSITE; PS51334; PRONE; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Cytoplasm; Guanine-nucleotide releasing factor; Membrane;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..515
FT /note="Rop guanine nucleotide exchange factor 12"
FT /id="PRO_0000423897"
FT DOMAIN 83..446
FT /note="PRONE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00663"
FT MOD_RES 510
FT /note="Phosphoserine"
FT /evidence="ECO:0000255"
FT MUTAGEN 458
FT /note="T->A,D: No effect on pollen tube growth."
FT /evidence="ECO:0000269|PubMed:18000057"
FT MUTAGEN 500
FT /note="S->A,D: No effect on pollen tube growth."
FT /evidence="ECO:0000269|PubMed:18000057"
FT MUTAGEN 510
FT /note="S->A: No effect on pollen tube growth."
FT /evidence="ECO:0000269|PubMed:18000057"
FT MUTAGEN 510
FT /note="S->D: Increased pollen tube growth."
FT /evidence="ECO:0000269|PubMed:18000057"
SQ SEQUENCE 515 AA; 58045 MW; DCD2755685CAA48A CRC64;
MVRASEQEQE TYRSRLFNFK WRNNDNNSAT RHNKSLSVET GLDEAATGSH DAEPLTIIHP
SQGPPLSRSA ADEAVLAALA ASQARERQLL ADMEQMKERF SKLLLGEDNS GGGKGVSSAL
ALSNAITNLA ASVFGEQRRL EPMPAERRAR WRKEIDWLLS VTDYVVEFAP SQQKNKDGTN
MEIMTTRQRT DLHMNIPALK KLDAMLIDCL ENFKDQSEFS YISKDSPDLD GKRNDEKWWI
PTVKVPPDGL SEASRRFLQY QKDCVNQVLK AAMAINAQVL FEMEIPESYI DSLPKNGRAS
LGDQMYKNIT VDFFDPDQFL SSMDMSSEHK IVDLKNRIEA SIIIWKRKMV YKDNKSSAPW
ASGVSLEKRE VFEERAETIL LILKQRYPGI SQSSLDISKI QFNEDVGQAV LESYSRILES
LAYTVLSRID DVLEADRAGN KRNTPLEAEE ETLVGSMTLS DFMGWDFDQA ANAELESKKD
LPDDPLIKEK LSVVTTKKTS YLETLGGVKS PTARH
