ROGFE_ARATH
ID ROGFE_ARATH Reviewed; 576 AA.
AC Q56WM6; Q8GWY8; Q9C6V5;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 96.
DE RecName: Full=Rop guanine nucleotide exchange factor 14;
DE Short=AtRopGEF14;
DE AltName: Full=Rho of plants guanine nucleotide exchange factor 14;
GN Name=ROPGEF14; OrderedLocusNames=At1g31650; ORFNames=F27M3.15;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY.
RX PubMed=15980860; DOI=10.1038/nature03883;
RA Berken A., Thomas C., Wittinghofer A.;
RT "A new family of RhoGEFs activates the Rop molecular switch in plants.";
RL Nature 436:1176-1180(2005).
RN [6]
RP INTERACTION WITH ARAC11/ROP1, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=16415208; DOI=10.1105/tpc.105.036434;
RA Gu Y., Li S., Lord E.M., Yang Z.;
RT "Members of a novel class of Arabidopsis Rho guanine nucleotide exchange
RT factors control Rho GTPase-dependent polar growth.";
RL Plant Cell 18:366-381(2006).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-435, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=19245862; DOI=10.1016/j.jprot.2009.02.004;
RA Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E.,
RA Rathjen J.P., Peck S.C.;
RT "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis
RT thaliana.";
RL J. Proteomics 72:439-451(2009).
CC -!- FUNCTION: Guanine-nucleotide exchange factor (GEF) that acts as an
CC activator of Rop (Rho of plants) GTPases by promoting the exchange of
CC GDP for GTP. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with ARAC11/ROP1. {ECO:0000269|PubMed:16415208}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:16415208}.
CC Note=Localizes to the apical region of the pollen tube plasma membrane.
CC -!- TISSUE SPECIFICITY: Expressed in pollen grains.
CC {ECO:0000269|PubMed:16415208}.
CC -!- DOMAIN: The PRONE (plant-specific Rop nucleotide exchanger) domain is
CC responsible for the GEF activity. {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG60161.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC074360; AAG60161.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE31380.1; -; Genomic_DNA.
DR EMBL; AK118553; BAC43154.1; -; mRNA.
DR EMBL; AK222010; BAD94650.1; -; mRNA.
DR RefSeq; NP_001320846.1; NM_001332971.1.
DR RefSeq; NP_174446.2; NM_102900.5.
DR AlphaFoldDB; Q56WM6; -.
DR SMR; Q56WM6; -.
DR BioGRID; 25287; 2.
DR DIP; DIP-59398N; -.
DR IntAct; Q56WM6; 1.
DR STRING; 3702.AT1G31650.1; -.
DR iPTMnet; Q56WM6; -.
DR PaxDb; Q56WM6; -.
DR PRIDE; Q56WM6; -.
DR ProteomicsDB; 228075; -.
DR EnsemblPlants; AT1G31650.1; AT1G31650.1; AT1G31650.
DR GeneID; 840052; -.
DR Gramene; AT1G31650.1; AT1G31650.1; AT1G31650.
DR KEGG; ath:AT1G31650; -.
DR Araport; AT1G31650; -.
DR TAIR; locus:2028701; AT1G31650.
DR eggNOG; ENOG502QPJS; Eukaryota.
DR HOGENOM; CLU_019073_3_0_1; -.
DR InParanoid; Q56WM6; -.
DR OrthoDB; 482824at2759; -.
DR PhylomeDB; Q56WM6; -.
DR PRO; PR:Q56WM6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q56WM6; baseline and differential.
DR Genevisible; Q56WM6; AT.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISS:TAIR.
DR GO; GO:0080092; P:regulation of pollen tube growth; IGI:TAIR.
DR InterPro; IPR005512; PRONE_dom.
DR InterPro; IPR038937; RopGEF.
DR PANTHER; PTHR33101; PTHR33101; 1.
DR Pfam; PF03759; PRONE; 1.
DR PROSITE; PS51334; PRONE; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Guanine-nucleotide releasing factor; Membrane;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..576
FT /note="Rop guanine nucleotide exchange factor 14"
FT /id="PRO_0000423899"
FT DOMAIN 127..500
FT /note="PRONE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00663"
FT REGION 273..300
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 491..520
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 435
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:19245862"
FT CONFLICT 260
FT /note="T -> R (in Ref. 3; BAC43154)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 576 AA; 64434 MW; 7DC1C9FC147CC375 CRC64;
MMLMRRRLAC CSREREISID FDEHEQDKMI TYYGLETCII NNQSYEEESG TSRGDGCLTD
SLDDDAFSSC SSSKDASSSF SSKWLPMKND EHSCDGLNLS GRSQHFDAKE KKKQGYGSSQ
HFDAKEKPGY VYCHLDVEAM KEKFSKLLLG EDVTGGCKGV QVALALSNAV THLATSIFGE
LWKLEPLCEE KKQKWRREMD WLLSPTNYMI ELVPSKQNDA NGRSLEIMTP KARADIHMNL
PALQKLDSML IETLDSMVNT EFWYSEIGSR AEGKNKSTSE SKRWWLPSPQ VPKPGLSNSG
RKKLLDKGKV VYQVFKATKA INENILLEMP VPIVIKEAIP KSGKNSLGDE LYKMLAVESA
TVDEIFISLN LGTEHAALET VNKLESAMFA WKERITEQGS NGKSPVRASW SFAKDPLSEI
GRNESLLNRA EALRTQIKSK HPNLPHSFLD ATKIQYDKDI GHAVLEAYSR TLANLAFRIL
SRMGEILKED SLSNPNSPAP PSCFPSSRDP YRTPERPLLS SRVRHSLTDD MNKADGTETG
LDFLFADAKA SNSVNTTPSR SSRLWCLSKV PSDTSP
