ROH1_ARATH
ID ROH1_ARATH Reviewed; 415 AA.
AC Q9CAK4;
DT 26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Protein ROH1 {ECO:0000303|PubMed:20618910};
GN Name=ROH1 {ECO:0000303|PubMed:20618910};
GN OrderedLocusNames=At1g63930 {ECO:0000312|Araport:AT1G63930};
GN ORFNames=T12P18.5 {ECO:0000312|EMBL:AAG52457.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH EXO70A1 AND EXO70C1, AND
RP TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=20618910; DOI=10.1111/j.1469-8137.2010.03372.x;
RA Kulich I., Cole R., Drdova E., Cvrckova F., Soukup A., Fowler J.,
RA Zarsky V.;
RT "Arabidopsis exocyst subunits SEC8 and EXO70A1 and exocyst interactor ROH1
RT are involved in the localized deposition of seed coat pectin.";
RL New Phytol. 188:615-625(2010).
CC -!- FUNCTION: Required for seed coat mucilage deposition.
CC {ECO:0000269|PubMed:20618910}.
CC -!- SUBUNIT: Interacts with EXO70A1 and EXO70C1.
CC {ECO:0000269|PubMed:20618910}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Mainly expressed in cells expanding in a polar
CC manner such as pollen and root hairs. {ECO:0000269|PubMed:20618910}.
CC -!- DISRUPTION PHENOTYPE: Reduced pectin deposition leading to reduced seed
CC coat mucilage thickness (PubMed:20618910). Characteristic pattern of
CC pectin deposition to the corners of seed coat volcano cells
CC (PubMed:20618910). {ECO:0000269|PubMed:20618910}.
CC -!- MISCELLANEOUS: 'Roh' means corner in Czech.
CC {ECO:0000305|PubMed:20618910}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC010852; AAG52457.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34167.1; -; Genomic_DNA.
DR EMBL; AK221458; BAD94543.1; -; mRNA.
DR EMBL; BT023455; AAY56446.1; -; mRNA.
DR EMBL; BT029011; ABI93920.1; -; mRNA.
DR PIR; D96664; D96664.
DR RefSeq; NP_176576.1; NM_105066.3.
DR AlphaFoldDB; Q9CAK4; -.
DR IntAct; Q9CAK4; 1.
DR STRING; 3702.AT1G63930.1; -.
DR PaxDb; Q9CAK4; -.
DR PRIDE; Q9CAK4; -.
DR ProteomicsDB; 177728; -.
DR EnsemblPlants; AT1G63930.1; AT1G63930.1; AT1G63930.
DR GeneID; 842696; -.
DR Gramene; AT1G63930.1; AT1G63930.1; AT1G63930.
DR KEGG; ath:AT1G63930; -.
DR Araport; AT1G63930; -.
DR TAIR; locus:2195513; AT1G63930.
DR eggNOG; ENOG502QUJZ; Eukaryota.
DR HOGENOM; CLU_060027_0_0_1; -.
DR InParanoid; Q9CAK4; -.
DR OMA; LVRHWQK; -.
DR OrthoDB; 1150244at2759; -.
DR PhylomeDB; Q9CAK4; -.
DR PRO; PR:Q9CAK4; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9CAK4; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0048354; P:mucilage biosynthetic process involved in seed coat development; IMP:TAIR.
DR GO; GO:0010214; P:seed coat development; IMP:TAIR.
DR InterPro; IPR008511; ROH1-like.
DR Pfam; PF05633; BPS1; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Glycoprotein; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..415
FT /note="Protein ROH1"
FT /id="PRO_0000448861"
FT TRANSMEM 263..283
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 184..219
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 196..219
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 183
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 195
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 234
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 415 AA; 45637 MW; 0BA156C38E9AAE70 CRC64;
MRPAQDNQGS FLGRISIRRN QFVDVNNEQE QEDLELFQKH IADRFTELLS PPQPPPSDEI
NTVASVAATE QIMSVTWLRK LMDVFLCCEA EFKAILLMGR DPTQISKPPF DRLVPEMLDR
SIKALDICTA VVNGIDSVRH YQRLAEIAVT ALEQRPLGDG NVRRAKRALA NLVVALSLED
KENVSGGGGG GGGGNKTTER SWSFGRRSGG SSAASKGGAT IGQLKSSSWA VGRNWSAAKQ
IHAMTANLTP PRGNEAAGLP QPMFIMSTVM VFVMWVLTAA VPCQERSGLA NHLPVPPKHL
NWAQSLIGIH EKIGDEWKKK EKKGSAGLME EMTRMEKLGH SLMEFADGFH YPAEKDAAES
AAVQVAEMAE ICRRMEEELV PLQQQIREVF HRIVRSRAEI LEVLEQAGKV SAPVV
