ID   ROK1_VANPO              Reviewed;         570 AA.
AC   A7TT88;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 1.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=ATP-dependent RNA helicase ROK1;
DE            EC=3.6.4.13;
GN   Name=ROK1; ORFNames=Kpol_274p7;
OS   Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS
OS   2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17) (Kluyveromyces polysporus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Vanderwaltozyma.
OX   NCBI_TaxID=436907;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 / NRRL
RC   Y-8283 / UCD 57-17;
RX   PubMed=17494770; DOI=10.1073/pnas.0608218104;
RA   Scannell D.R., Frank A.C., Conant G.C., Byrne K.P., Woolfit M., Wolfe K.H.;
RT   "Independent sorting-out of thousands of duplicated gene pairs in two yeast
RT   species descended from a whole-genome duplication.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:8397-8402(2007).
CC   -!- FUNCTION: ATP-dependent RNA helicase involved in 40S ribosomal subunit
CC       biogenesis. Required for the processing and cleavage of 35S pre-rRNA at
CC       sites A0, A1, and A2, leading to mature 18S rRNA.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBUNIT: Interacts with the U3 snoRNA and is associated with the 90S
CC       and 40S pre-ribosomes. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC   -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC       family of RNA helicases and controls ATP binding and hydrolysis.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX52/ROK1
CC       subfamily. {ECO:0000305}.
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DR   EMBL; DS480550; EDO14524.1; -; Genomic_DNA.
DR   RefSeq; XP_001642382.1; XM_001642332.1.
DR   AlphaFoldDB; A7TT88; -.
DR   SMR; A7TT88; -.
DR   STRING; 436907.A7TT88; -.
DR   PRIDE; A7TT88; -.
DR   EnsemblFungi; EDO14524; EDO14524; Kpol_274p7.
DR   GeneID; 5542524; -.
DR   KEGG; vpo:Kpol_274p7; -.
DR   eggNOG; KOG0344; Eukaryota.
DR   HOGENOM; CLU_003041_1_4_1; -.
DR   InParanoid; A7TT88; -.
DR   OMA; IRAQHRI; -.
DR   OrthoDB; 400908at2759; -.
DR   PhylomeDB; A7TT88; -.
DR   Proteomes; UP000000267; Unassembled WGS sequence.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030490; P:maturation of SSU-rRNA; IEA:InterPro.
DR   CDD; cd17957; DEADc_DDX52; 1.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR044764; DDX52/Rok1_DEADc.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus;
KW   Reference proteome; Ribosome biogenesis; RNA-binding; rRNA processing.
FT   CHAIN           1..570
FT                   /note="ATP-dependent RNA helicase ROK1"
FT                   /id="PRO_0000310241"
FT   DOMAIN          162..342
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          353..515
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   REGION          69..96
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           131..159
FT                   /note="Q motif"
FT   MOTIF           289..292
FT                   /note="DEAD box"
FT   BINDING         175..182
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ   SEQUENCE   570 AA;  64670 MW;  22C1F012325E7495 CRC64;
     MDIFRLLTRG ASVKKDPKNK NIGDAVENAK ASNIEKNDKI AAENQITKEL DFFHNKRIIN
     KVNYNKAKET HAEDKEDKDN DNEEDEIKEE ESLQYQKPLI TNDEEAKLLR KSNKSNVSGE
     DIPLPIGSFE DLITRFSFDK RLLNNLILNH FTEPTPIQCE AIPLALNNRD MLACAPTGSG
     KTLAFLIPLI QQVINDKDTQ GLKGLIISPT KELANQIFLE CIKLSNRIFL DKKRPLQVAL
     LSKSLSAKLR NKVISDKKYD IIVSTPLRLI DVVKNEALDL SQVKHLIFDE ADKLFDKTFV
     EQSDDILSSC SHSSLRKSMF SATIPSNVEE IAKSIMMDPV RVIIGHKEAA NTSIEQKLVF
     CGNEEGKLIA IKQLVQEGEF KPPVIIFLES ITRAKALYHE MMYDSLNVDV IHAERTQVQR
     NKIIERFKSG DLWCLITTDV LARGVDFKGV NLVINYDVPR TAQAYVHRIG RTGRGGRSGK
     AVTFYTKEDS IAIKPIINVM KQSGSEISEW MEKVSKMTKK EKELVKKGRA HSERKQISTV
     PKIDKIKRKR KLEMIQASKK RKAIETKETE