ID   ROLC_TOBAC              Reviewed;         180 AA.
AC   Q43592;
DT   06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 47.
DE   RecName: Full=Cytokinin-beta-glucosidase;
DE            EC=3.2.1.-;
DE   AltName: Full=Protein ROL C;
DE            Short=trolC;
GN   Name=TROLC;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, AND INDUCTION BY
RP   AUXIN AND CYTOKININ.
RC   STRAIN=cv. Havana 425; TISSUE=Leaf;
RX   PubMed=7500950; DOI=10.1007/bf00290526;
RA   Meyer A.D., Ichikawa T., Meins F. Jr.;
RT   "Horizontal gene transfer: regulated expression of a tobacco homologue of
RT   the Agrobacterium rhizogenes rolC gene.";
RL   Mol. Gen. Genet. 249:265-273(1995).
CC   -!- FUNCTION: Hydrolyzes cytokinin glucosides thus liberating free
CC       cytokinins. {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Accumulates in young leaves and shoot tips.
CC       {ECO:0000269|PubMed:7500950}.
CC   -!- INDUCTION: Down-regulated by auxin but induced by cytokinin.
CC       {ECO:0000269|PubMed:7500950}.
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DR   EMBL; X91881; CAA62988.1; -; Genomic_DNA.
DR   EMBL; FN667969; CBJ56560.1; -; Genomic_DNA.
DR   PIR; S60204; S60204.
DR   AlphaFoldDB; Q43592; -.
DR   SMR; Q43592; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0008422; F:beta-glucosidase activity; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0071365; P:cellular response to auxin stimulus; IEP:UniProtKB.
DR   GO; GO:0071368; P:cellular response to cytokinin stimulus; IEP:UniProtKB.
DR   GO; GO:0009691; P:cytokinin biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR006065; Glyco_hydro_41.
DR   InterPro; IPR006064; Glycosidase.
DR   Pfam; PF02027; RolB_RolC; 1.
DR   PRINTS; PR00746; GLHYDRLASE41.
PE   2: Evidence at transcript level;
KW   Cytokinin biosynthesis; Glycosidase; Hydrolase; Reference proteome.
FT   CHAIN           1..180
FT                   /note="Cytokinin-beta-glucosidase"
FT                   /id="PRO_0000421061"
SQ   SEQUENCE   180 AA;  20045 MW;  50F3EB5EC00CB937 CRC64;
     MAEDNLCALF FKLTVQGVTC SDGLKKHMEN AEDELKPLLE HGPQGESMDI DEEEVSGAKR
     LLYLYVDCPT MMRCFFGRSM PYNCRGGTLL TDLPPYHADA SPHEVSRGLG KATDFFSYED
     AIRSAYFAAF SFSGPVRKLD QQLELTSTRG EPLTFSLYAR SPGRLEPGEL VRHGECKFAG