RON22_TOXGM
ID RON22_TOXGM Reviewed; 1167 AA.
AC B6KLP1; S8G7M8;
DT 29-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2013, sequence version 2.
DT 25-MAY-2022, entry version 45.
DE RecName: Full=Rhoptry neck protein 2-like protein 2;
DE AltName: Full=Sporozoite-specific paralog of RON2;
DE Short=SporoRON2;
DE Flags: Precursor;
GN Name=RON2L2; ORFNames=TGME49_065120;
OS Toxoplasma gondii (strain ATCC 50611 / Me49).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia;
OC Eucoccidiorida; Eimeriorina; Sarcocystidae; Toxoplasma.
OX NCBI_TaxID=508771;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 50611 / Me49;
RA Lorenzi H., Caler E., Galinsky K., Schobel S., Brunk B.P., Roos D.S.,
RA Paulsen I.;
RT "Annotation of Toxoplasma gondii ME49.";
RL Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND INDUCTION.
RX PubMed=22279555; DOI=10.1371/journal.pone.0029955;
RA Fritz H.M., Bowyer P.W., Bogyo M., Conrad P.A., Boothroyd J.C.;
RT "Proteomic analysis of fractionated Toxoplasma oocysts reveals clues to
RT their environmental resistance.";
RL PLoS ONE 7:E29955-E29955(2012).
RN [3]
RP INDUCTION.
RX PubMed=22347997; DOI=10.1371/journal.pone.0029998;
RA Fritz H.M., Buchholz K.R., Chen X., Durbin-Johnson B., Rocke D.M.,
RA Conrad P.A., Boothroyd J.C.;
RT "Transcriptomic analysis of toxoplasma development reveals many novel
RT functions and structures specific to sporozoites and oocysts.";
RL PLoS ONE 7:E29998-E29998(2012).
CC -!- FUNCTION: May play a role in host cell invasion. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Host cell membrane
CC {ECO:0000250}; Single-pass type I membrane protein {ECO:0000305};
CC Cytoplasmic side {ECO:0000250}. Note=Initially localizes to rhoptries,
CC specialized secretory organelles of apicomplexan parasites important
CC for host cell invasion. Upon host invasion by tachyzoites, the protein
CC is injected into the host cell, where it spans the host cell membrane
CC (By similarity). {ECO:0000250}.
CC -!- INDUCTION: Readily detected in oocysts (at protein level). Specifically
CC up-regulated in sporozoites, but not in tachyzoites and bradyzoites.
CC {ECO:0000269|PubMed:22279555, ECO:0000269|PubMed:22347997}.
CC -!- SIMILARITY: Belongs to the apicomplexan parasites RON2 family.
CC {ECO:0000305}.
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DR EMBL; KE138832; EPT27750.1; -; Genomic_DNA.
DR RefSeq; XP_018636309.1; XM_018781392.1.
DR PDB; 3ZLD; X-ray; 3.10 A; B=999-1034.
DR PDBsum; 3ZLD; -.
DR AlphaFoldDB; B6KLP1; -.
DR SMR; B6KLP1; -.
DR PRIDE; B6KLP1; -.
DR EnsemblProtists; TGME49_265120-t26_1; TGME49_265120-t26_1; TGME49_265120.
DR GeneID; 7900663; -.
DR KEGG; tgo:TGME49_265120; -.
DR VEuPathDB; ToxoDB:TGME49_265120; -.
DR HOGENOM; CLU_441151_0_0_1; -.
DR PhylomeDB; B6KLP1; -.
DR Proteomes; UP000001529; Chromosome IX.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW 3D-structure; Disulfide bond; Host cell membrane; Host membrane; Membrane;
KW Secreted; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..1167
FT /note="Rhoptry neck protein 2-like protein 2"
FT /id="PRO_0000422340"
FT TOPO_DOM 21..977
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 978..998
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 999..1167
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REGION 55..94
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 1015..1026
FT /evidence="ECO:0000250"
FT HELIX 1001..1005
FT /evidence="ECO:0007829|PDB:3ZLD"
FT TURN 1006..1008
FT /evidence="ECO:0007829|PDB:3ZLD"
FT STRAND 1014..1018
FT /evidence="ECO:0007829|PDB:3ZLD"
FT TURN 1019..1022
FT /evidence="ECO:0007829|PDB:3ZLD"
FT STRAND 1023..1027
FT /evidence="ECO:0007829|PDB:3ZLD"
SQ SEQUENCE 1167 AA; 131395 MW; 60C2A044987BB747 CRC64;
MSSNLAFLSL SLAESTASLG KSLEETRTRL TEKLKLVAGM ALGFDQFAMH QTATGPGLSV
EGKQTEQMSR KSAEDTRASS LSSDPDDGRA AQLAGQKGHV TPCIAGLIAT SNPFLFTHML
ALGAAYLGYD KYFGDGTSEL LPFYGTRTLL STLSPLDTPR LLDSMAAADR LSPKRSILSR
FSRRKRSLDA PAPEPSGWDQ KRKSRLPLGS LRLVLDILDR HLEALTTYVQ QQIEIFRQAG
VPLEASVTMT HNLNRLHVTR CQSRNNQMVI PCRWKDTTFF EELSNETNPG LTDEEQLEKR
VKFHKLENAF NTVTGLGALE AMLDEDSVLL GGPAEESVAR LYSDFHLGAA MNNWYFYDAL
NINKMLSRFQ KVGTKVLKQY GLENLMNLAT SHQAGSTASW DRTDIDTKLA KHHHYGSALR
VRLYIMALLP QFTPHREGIL SPVDFIREAF AGERSYAMST ARLKPLLSDD LVILGVPKGF
SFLWLFEFLL EEDSPNALVA KRRALKRIPN WTKRLFSHSR SIPMLFRQFY KALNAGVFAK
ARRRTLDRSK TLLSRQLPEG WRDIVMDAFA FTAHSAALMQ VVNYHSLFRD RTLNDLQEAS
YLATPPAFSI EDQSVMKRCD EQIYEWSRFG FSPDVLEADL HNETFKGKWE NLRLETMPTP
DTHQWWRRLH RAILDSLEIY QAHERHLKGM SDNLYTLVED TIKNLQDGSS RDDTIFFGRI
ARGARESVGQ KMWRGVKTFF LDLVRDVPGS DHAVWFGVGF NFPRIFQILR KMKEIALHAA
GDQGSAILLD EAFVELVQET VAVRAQTYRR QPPTTLRNIG VIGLRPDYAN LDTEQRSMEY
QLSMCADHCV SLWVQILGFI YPYVLNPSLL TDYEKSFGTG HAIKKLDDPS YVNSFRYIFA
NDASLNFFEH NTPQDTRKAL VSLKSGQAFM YANMMRFAGI AFEQLSMPYL AVSLQRQAPF
MGQMVKDWVA KRSRSRKLAI VSVLSLGLIF AYTLLSALDI AQFLTDSGMK AIEDCSWNPI
MQQMACVVVA GSGSLVAPTF AALSDVFKVG LYSGIGSTLI AASVVGNAYM IIRSQSRTIL
RTEMAIKNVA VKLWKQMRKY FSWVTRFTKR RKAILSTMMA RATALAKNKK PRSETAATMT
RSGDSVMDML LQGVSPATPQ RAQDGSR
