RON4_TOXGO
ID RON4_TOXGO Reviewed; 984 AA.
AC B6KJ32; B9PI02; B9Q636; Q27914; Q45WA5; Q6XNE6;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 2.
DT 25-MAY-2022, entry version 41.
DE RecName: Full=Rhoptry neck protein 4;
DE AltName: Full=Tg65;
DE Flags: Precursor;
GN Name=RON4;
OS Toxoplasma gondii.
OC Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia;
OC Eucoccidiorida; Eimeriorina; Sarcocystidae; Toxoplasma.
OX NCBI_TaxID=5811;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND SUBCELLULAR LOCATION.
RC STRAIN=RH;
RX PubMed=16002398; DOI=10.1074/jbc.m504158200;
RA Bradley P.J., Ward C., Cheng S.J., Alexander D.L., Coller S., Coombs G.H.,
RA Dunn J.D., Ferguson D.J., Sanderson S.J., Wastling J.M., Boothroyd J.C.;
RT "Proteomic analysis of rhoptry organelles reveals many novel constituents
RT for host-parasite interactions in Toxoplasma gondii.";
RL J. Biol. Chem. 280:34245-34258(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 2).
RC STRAIN=RH;
RX PubMed=16149193; DOI=10.1017/s0031182005007559;
RA Henriquez F.L., Nickdel M.B., McLeod R., Lyons R.E., Lyons K.,
RA Dubremetz J.F., Grigg M.E., Samuel B.U., Roberts C.W.;
RT "Toxoplasma gondii dense granule protein 3 (GRA3) is a type I transmembrane
RT protein that possesses a cytoplasmic dilysine (KKXX) endoplasmic reticulum
RT (ER) retrieval motif.";
RL Parasitology 131:169-179(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 910-984 (ISOFORMS 1/2).
RC STRAIN=RH; TISSUE=Tachyzoite;
RX PubMed=7739670; DOI=10.1016/0166-6851(94)90169-4;
RA Bermudes D., Dubremetz J.-F., Achbarou A., Joiner K.A.;
RT "Cloning of a cDNA encoding the dense granule protein GRA3 from Toxoplasma
RT gondii.";
RL Mol. Biochem. Parasitol. 68:247-257(1994).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16002398}.
CC Parasitophorous vacuole membrane {ECO:0000269|PubMed:16002398}.
CC Note=Located prominently at the junction of the body and neck portion
CC of the rhoptry organelle in tachyzoites and bradyzoites. Upon
CC infection, associates with the parasitophorous vacuolar membrane (PVM).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=B6KJ32-1; Sequence=Displayed;
CC Name=2;
CC IsoId=B6KJ32-2; Sequence=VSP_039809;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC13772.1; Type=Miscellaneous discrepancy; Note=Chimeric cDNA, matches the C-terminus.; Evidence={ECO:0000305};
CC Sequence=AAC13773.1; Type=Miscellaneous discrepancy; Note=Chimeric cDNA, matches the C-terminus.; Evidence={ECO:0000305};
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DR EMBL; DQ096566; AAZ38166.1; -; mRNA.
DR EMBL; AY223804; AAP06935.1; -; mRNA.
DR EMBL; AY223805; AAP06936.1; -; Genomic_DNA.
DR EMBL; U13771; AAC13772.1; ALT_SEQ; mRNA.
DR EMBL; U13771; AAC13773.1; ALT_SEQ; mRNA.
DR AlphaFoldDB; B6KJ32; -.
DR PRIDE; B6KJ32; -.
DR VEuPathDB; ToxoDB:TGARI_229010A; -.
DR VEuPathDB; ToxoDB:TGARI_229010B; -.
DR VEuPathDB; ToxoDB:TGCAST_229010; -.
DR VEuPathDB; ToxoDB:TGCOUG_229010A; -.
DR VEuPathDB; ToxoDB:TGCOUG_229010B; -.
DR VEuPathDB; ToxoDB:TGDOM2_229010A; -.
DR VEuPathDB; ToxoDB:TGDOM2_229010B; -.
DR VEuPathDB; ToxoDB:TGFOU_402780; -.
DR VEuPathDB; ToxoDB:TGGT1_229010; -.
DR VEuPathDB; ToxoDB:TGMAS_229010; -.
DR VEuPathDB; ToxoDB:TGME49_229010; -.
DR VEuPathDB; ToxoDB:TGP89_229010; -.
DR VEuPathDB; ToxoDB:TGPRC2_229010; -.
DR VEuPathDB; ToxoDB:TGRH88_073840; -.
DR VEuPathDB; ToxoDB:TGRUB_430050; -.
DR VEuPathDB; ToxoDB:TGRUB_430060; -.
DR VEuPathDB; ToxoDB:TGVAND_229010; -.
DR VEuPathDB; ToxoDB:TGVEG_229010; -.
DR InParanoid; B6KJ32; -.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0020005; C:symbiont-containing vacuole membrane; IEA:UniProtKB-SubCell.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycoprotein; Membrane; Repeat; Secreted; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..984
FT /note="Rhoptry neck protein 4"
FT /id="PRO_0000398814"
FT REPEAT 129..172
FT /note="1"
FT REPEAT 258..301
FT /note="2"
FT REGION 24..300
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 882..984
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 64..107
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 133..160
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 183..207
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 235..289
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 937..964
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 965..984
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 81
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 390
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 780
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 925
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..394
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16149193"
FT /id="VSP_039809"
FT CONFLICT 892
FT /note="M -> V (in Ref. 1; AAZ38166)"
FT /evidence="ECO:0000305"
FT CONFLICT 911
FT /note="T -> A (in Ref. 1; AAZ38166)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 984 AA; 107238 MW; 1CD7AD28A68789F4 CRC64;
MAIKNTLTGS GLLVLLTLAC GTTVQSSPPT PAPRMYPNMN ERPLSAESRL TPGTYRSELH
IDLKSPQKTA SQSSLAPTGD NNSKVESIIA GSDTTPRSAE GTSESPPVPQ LGTPPRPAPR
RSKGEGERQP PTAAPRTSRS VDTGSGSDAS TEQQAGGQKV VTPIPASKGI YPSLDELRRS
QESNVDAGSG TSTNEGGGTS EGPQVPQSGI PPRTGRRRAK ARNRKRHPTP ASRESSSEDE
NQPPTTASRP SNGEGESQPP TAAPRTSRSV DTGSGSDAST EQQAGGQKVV TPIPASKGIY
PNLDELRQTQ EGEESAPQPA DVTWFSMSVP MGVVDRRVSR ALKEQFFQFL QHLSADYPKQ
VQTVYEFLGW VADKLPENEE EVQMFIDALN TTEAMVGKAA RWIFKAIPER ERETIYSSFY
QMFRDKLPKK FFETAEGMNP DVGQYFSAEE PVAVTPEIPA KSEEDSEAAE TPTPLRRQAN
VAAQVLHPLP AKGVTRREWI PWPKMQNAIS KAHGPLTRVP EWTPVTGSCA LGDGYTDIDV
TKATTDVLFR ITLLILQQIR RKKTERGKLE DDQALVALCS AAGAFVDAWQ HQQQALILED
PGTPKAHAQL IERLRNAGKY FMKSYDETTG ESDHQQWKKN KAEVSKLGKS ALMKSCVKYI
KASGDVATRP FDSGTAKYPS RSLYGGIANT LETPFADSEA VAKAVHDYAK EHKKPEKLVG
LCGALQISGY FKKCFSDAGR LSSVSFFHQH VDGASVLVRT LARERPIGRH ALSQAICDPN
ISAQYFEGAF RLFSSTVSQE WEKENLFAQL ASWTGKEMVL APSLEEQAAV PPAQPAYETV
YGDEEDRIYR IHVSGRHSSP QEVLYVGGIP STVKPQQVHV LGPTVSDESR RMIHPVRHRS
RTAPSSEAAS TAAESSDEDP LPAENATAFL GVTPQEEESD AYKHTLDFDA VSPRKNKNKE
NRISAPLKQS DTLIEESTSK TSEL
