ROP1A_HUMAN
ID ROP1A_HUMAN Reviewed; 212 AA.
AC Q9HAT0; D3DN99; Q9UF38;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2001, sequence version 2.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Ropporin-1A;
DE AltName: Full=Cancer/testis antigen 91;
DE Short=CT91;
DE AltName: Full=Rhophilin-associated protein 1A;
GN Name=ROPN1; Synonyms=ROPN1A;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RA Zhou Z.M., Li J.M., Sha J.H.;
RT "Cloning of a human sperm-specific binding protein of rhophilin and
RT function in spermatogenesis.";
RL Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Testis;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP TISSUE SPECIFICITY, POSSIBLE INTERACTION WITH SPA17, DEVELOPMENTAL STAGE,
RP AND IDENTIFICATION AS A CANCER/TESTIS ANTIGEN.
RX PubMed=17551920; DOI=10.1002/ijc.22842;
RA Li Z., Li W., Meklat F., Wang Z., Zhang J., Zhang Y., Lim S.H.;
RT "A yeast two-hybrid system using Sp17 identified ropporin as a novel
RT cancer-testis antigen in hematologic malignancies.";
RL Int. J. Cancer 121:1507-1511(2007).
CC -!- FUNCTION: Important for male fertility. With ROPN1L, involved in
CC fibrous sheath integrity and sperm motility, plays a role in PKA-
CC dependent signaling processes required for spermatozoa capacitation.
CC {ECO:0000250|UniProtKB:Q9ESG2}.
CC -!- SUBUNIT: Homodimer. Interacts with AKAP3 and RHPN1 (By similarity). May
CC interact with SPA17 (PubMed:17551920). Interacts with FSCB; the
CC interaction increases upon spermatozoa capacitation conditions (By
CC similarity). {ECO:0000250|UniProtKB:Q9BZX4,
CC ECO:0000250|UniProtKB:Q9ESG2, ECO:0000269|PubMed:17551920}.
CC -!- INTERACTION:
CC Q9HAT0; P0CW23: AKAIN1; NbExp=3; IntAct=EBI-1378139, EBI-18397742;
CC Q9HAT0; Q86UN6: AKAP14; NbExp=3; IntAct=EBI-1378139, EBI-2119626;
CC Q9HAT0; P24588: AKAP5; NbExp=3; IntAct=EBI-1378139, EBI-703640;
CC Q9HAT0; O43687-2: AKAP7; NbExp=9; IntAct=EBI-1378139, EBI-10185182;
CC Q9HAT0; Q9BXS5: AP1M1; NbExp=3; IntAct=EBI-1378139, EBI-541426;
CC Q9HAT0; Q32M84-2: BTBD16; NbExp=3; IntAct=EBI-1378139, EBI-11961432;
CC Q9HAT0; Q16543: CDC37; NbExp=3; IntAct=EBI-1378139, EBI-295634;
CC Q9HAT0; Q8IYX8-2: CEP57L1; NbExp=3; IntAct=EBI-1378139, EBI-10181988;
CC Q9HAT0; P61024: CKS1B; NbExp=6; IntAct=EBI-1378139, EBI-456371;
CC Q9HAT0; Q6PJW8: CNST; NbExp=5; IntAct=EBI-1378139, EBI-750390;
CC Q9HAT0; Q8NFT6-2: DBF4B; NbExp=6; IntAct=EBI-1378139, EBI-12205861;
CC Q9HAT0; P19447: ERCC3; NbExp=3; IntAct=EBI-1378139, EBI-1183307;
CC Q9HAT0; Q9BQ89: FAM110A; NbExp=3; IntAct=EBI-1378139, EBI-1752811;
CC Q9HAT0; Q9UHL3: FAM153A; NbExp=3; IntAct=EBI-1378139, EBI-2834075;
CC Q9HAT0; P0C7A2-2: FAM153B; NbExp=5; IntAct=EBI-1378139, EBI-12940382;
CC Q9HAT0; Q3B820: FAM161A; NbExp=3; IntAct=EBI-1378139, EBI-719941;
CC Q9HAT0; Q86YD7: FAM90A1; NbExp=3; IntAct=EBI-1378139, EBI-6658203;
CC Q9HAT0; Q9NVN8: GNL3L; NbExp=7; IntAct=EBI-1378139, EBI-746682;
CC Q9HAT0; Q92917: GPKOW; NbExp=6; IntAct=EBI-1378139, EBI-746309;
CC Q9HAT0; Q9BTB7: HNRPUL1; NbExp=3; IntAct=EBI-1378139, EBI-10298318;
CC Q9HAT0; Q2KHM9: KIAA0753; NbExp=3; IntAct=EBI-1378139, EBI-2805604;
CC Q9HAT0; Q6PJI1: KIF9; NbExp=3; IntAct=EBI-1378139, EBI-10254090;
CC Q9HAT0; Q96BZ8: LENG1; NbExp=8; IntAct=EBI-1378139, EBI-726510;
CC Q9HAT0; P61968: LMO4; NbExp=3; IntAct=EBI-1378139, EBI-2798728;
CC Q9HAT0; Q8TBB1: LNX1; NbExp=5; IntAct=EBI-1378139, EBI-739832;
CC Q9HAT0; Q96HA8: NTAQ1; NbExp=5; IntAct=EBI-1378139, EBI-741158;
CC Q9HAT0; Q9NRD5: PICK1; NbExp=3; IntAct=EBI-1378139, EBI-79165;
CC Q9HAT0; Q99959-2: PKP2; NbExp=3; IntAct=EBI-1378139, EBI-10987518;
CC Q9HAT0; Q9BUI4: POLR3C; NbExp=3; IntAct=EBI-1378139, EBI-5452779;
CC Q9HAT0; Q9NQX0: PRDM6; NbExp=3; IntAct=EBI-1378139, EBI-11320284;
CC Q9HAT0; Q13131: PRKAA1; NbExp=4; IntAct=EBI-1378139, EBI-1181405;
CC Q9HAT0; P25786: PSMA1; NbExp=6; IntAct=EBI-1378139, EBI-359352;
CC Q9HAT0; Q9HAT0: ROPN1; NbExp=6; IntAct=EBI-1378139, EBI-1378139;
CC Q9HAT0; Q14D33: RTP5; NbExp=6; IntAct=EBI-1378139, EBI-10217913;
CC Q9HAT0; O00560: SDCBP; NbExp=6; IntAct=EBI-1378139, EBI-727004;
CC Q9HAT0; Q53GC0: SERTAD1; NbExp=3; IntAct=EBI-1378139, EBI-2826300;
CC Q9HAT0; O60504: SORBS3; NbExp=3; IntAct=EBI-1378139, EBI-741237;
CC Q9HAT0; Q15506: SPA17; NbExp=9; IntAct=EBI-1378139, EBI-1377865;
CC Q9HAT0; Q9NZD8: SPG21; NbExp=3; IntAct=EBI-1378139, EBI-742688;
CC Q9HAT0; Q96C24: SYTL4; NbExp=3; IntAct=EBI-1378139, EBI-747142;
CC Q9HAT0; O75382: TRIM3; NbExp=3; IntAct=EBI-1378139, EBI-2129889;
CC Q9HAT0; Q3SY00: TSGA10IP; NbExp=3; IntAct=EBI-1378139, EBI-10241197;
CC Q9HAT0; Q63HK5: TSHZ3; NbExp=3; IntAct=EBI-1378139, EBI-9053916;
CC Q9HAT0; O75386: TULP3; NbExp=3; IntAct=EBI-1378139, EBI-5357290;
CC Q9HAT0; Q96B02: UBE2W; NbExp=3; IntAct=EBI-1378139, EBI-716589;
CC Q9HAT0; Q96FI0: UBE2W; NbExp=3; IntAct=EBI-1378139, EBI-10285774;
CC Q9HAT0; Q96AX1: VPS33A; NbExp=3; IntAct=EBI-1378139, EBI-2527283;
CC Q9HAT0; B7ZMN2: XKR3; NbExp=3; IntAct=EBI-1378139, EBI-10176176;
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum
CC {ECO:0000250|UniProtKB:Q9ESG2}. Note=In the sperm tail, found in the
CC principal piece and in the cytoplasmic droplet located at the distal
CC end of the midpiece. Inner surface of the fibrous sheath.
CC {ECO:0000250|UniProtKB:Q9ESG2}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9HAT0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9HAT0-2; Sequence=VSP_028743, VSP_028744;
CC -!- TISSUE SPECIFICITY: Testis specific in adult. Overexpressed in
CC hematologic tumor cells. {ECO:0000269|PubMed:17551920}.
CC -!- DEVELOPMENTAL STAGE: Expressed in fetal liver.
CC {ECO:0000269|PubMed:17551920}.
CC -!- DOMAIN: The RIIa domain mediates interaction with AKAP3. {ECO:0000250}.
CC -!- PTM: Sumoylated, sumoylation decreases upon spermatozoa capacitation
CC conditions. {ECO:0000250|UniProtKB:Q9ESG2}.
CC -!- MISCELLANEOUS: 'Ropporin' comes from the Japanese word 'oppo' which
CC means 'tail'.
CC -!- SIMILARITY: Belongs to the ropporin family. {ECO:0000305}.
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DR EMBL; AF303889; AAG27712.2; -; mRNA.
DR EMBL; AL133624; CAB63750.2; -; mRNA.
DR EMBL; CH471052; EAW79425.1; -; Genomic_DNA.
DR EMBL; CH471052; EAW79426.1; -; Genomic_DNA.
DR EMBL; CH471052; EAW79427.1; -; Genomic_DNA.
DR EMBL; BC132744; AAI32745.1; -; mRNA.
DR EMBL; BC132746; AAI32747.1; -; mRNA.
DR CCDS; CCDS3026.1; -. [Q9HAT0-1]
DR RefSeq; NP_001304703.1; NM_001317774.1. [Q9HAT0-1]
DR RefSeq; NP_060048.2; NM_017578.3. [Q9HAT0-1]
DR RefSeq; XP_011511235.1; XM_011512933.2. [Q9HAT0-1]
DR RefSeq; XP_011511236.1; XM_011512934.2. [Q9HAT0-1]
DR AlphaFoldDB; Q9HAT0; -.
DR BioGRID; 120138; 62.
DR IntAct; Q9HAT0; 56.
DR MINT; Q9HAT0; -.
DR STRING; 9606.ENSP00000184183; -.
DR iPTMnet; Q9HAT0; -.
DR PhosphoSitePlus; Q9HAT0; -.
DR BioMuta; ROPN1; -.
DR DMDM; 74718687; -.
DR MassIVE; Q9HAT0; -.
DR PaxDb; Q9HAT0; -.
DR PeptideAtlas; Q9HAT0; -.
DR PRIDE; Q9HAT0; -.
DR ProteomicsDB; 81425; -. [Q9HAT0-1]
DR Antibodypedia; 32969; 58 antibodies from 14 providers.
DR DNASU; 54763; -.
DR Ensembl; ENST00000184183.8; ENSP00000184183.4; ENSG00000065371.18. [Q9HAT0-1]
DR Ensembl; ENST00000405845.8; ENSP00000385919.3; ENSG00000065371.18. [Q9HAT0-1]
DR Ensembl; ENST00000459660.5; ENSP00000420590.1; ENSG00000065371.18. [Q9HAT0-2]
DR Ensembl; ENST00000479867.1; ENSP00000420567.1; ENSG00000065371.18. [Q9HAT0-2]
DR Ensembl; ENST00000484329.1; ENSP00000419205.1; ENSG00000065371.18. [Q9HAT0-2]
DR Ensembl; ENST00000495093.1; ENSP00000417379.1; ENSG00000065371.18. [Q9HAT0-2]
DR Ensembl; ENST00000620893.4; ENSP00000483603.1; ENSG00000065371.18. [Q9HAT0-1]
DR GeneID; 54763; -.
DR KEGG; hsa:54763; -.
DR MANE-Select; ENST00000405845.8; ENSP00000385919.3; NM_001317774.2; NP_001304703.1.
DR UCSC; uc003eha.4; human. [Q9HAT0-1]
DR CTD; 54763; -.
DR DisGeNET; 54763; -.
DR GeneCards; ROPN1; -.
DR HGNC; HGNC:17692; ROPN1.
DR HPA; ENSG00000065371; Tissue enriched (testis).
DR neXtProt; NX_Q9HAT0; -.
DR OpenTargets; ENSG00000065371; -.
DR PharmGKB; PA134952827; -.
DR VEuPathDB; HostDB:ENSG00000065371; -.
DR eggNOG; ENOG502R2JI; Eukaryota.
DR GeneTree; ENSGT00390000012731; -.
DR HOGENOM; CLU_069829_1_0_1; -.
DR InParanoid; Q9HAT0; -.
DR OMA; QWASDYF; -.
DR PhylomeDB; Q9HAT0; -.
DR TreeFam; TF105421; -.
DR PathwayCommons; Q9HAT0; -.
DR Reactome; R-HSA-5666185; RHO GTPases Activate Rhotekin and Rhophilins.
DR SignaLink; Q9HAT0; -.
DR BioGRID-ORCS; 54763; 17 hits in 1019 CRISPR screens.
DR GenomeRNAi; 54763; -.
DR Pharos; Q9HAT0; Tbio.
DR PRO; PR:Q9HAT0; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; Q9HAT0; protein.
DR Bgee; ENSG00000065371; Expressed in sperm and 82 other tissues.
DR ExpressionAtlas; Q9HAT0; baseline and differential.
DR Genevisible; Q9HAT0; HS.
DR GO; GO:0005737; C:cytoplasm; IDA:BHF-UCL.
DR GO; GO:0031514; C:motile cilium; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; HDA:UniProtKB.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0044782; P:cilium organization; ISS:UniProtKB.
DR GO; GO:0030317; P:flagellated sperm motility; ISS:UniProtKB.
DR GO; GO:0061512; P:protein localization to cilium; ISS:UniProtKB.
DR GO; GO:0001932; P:regulation of protein phosphorylation; ISS:UniProtKB.
DR GO; GO:0048240; P:sperm capacitation; ISS:UniProtKB.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell projection; Cilium; Flagellum; Phosphoprotein;
KW Reference proteome; Ubl conjugation.
FT CHAIN 1..212
FT /note="Ropporin-1A"
FT /id="PRO_0000307392"
FT DOMAIN 12..49
FT /note="RIIa"
FT REGION 209..212
FT /note="Interaction with RHPN1"
FT /evidence="ECO:0000250"
FT MOD_RES 56
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q4KLL5"
FT VAR_SEQ 39..58
FT /note="DYFEALSRGETPPVRERSER -> EYVLLSRLHPLEDGRRQRVL (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_028743"
FT VAR_SEQ 59..212
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_028744"
SQ SEQUENCE 212 AA; 23893 MW; B50D2859B464F6CC CRC64;
MAQTDKPTCI PPELPKMLKE FAKAAIRVQP QDLIQWAADY FEALSRGETP PVRERSERVA
LCNRAELTPE LLKILHSQVA GRLIIRAEEL AQMWKVVNLP TDLFNSVMNV GRFTEEIEWL
KFLALACSAL GVTITKTLKI VCEVLSCDHN GGSPRIPFST FQFLYTYIAK VDGEISASHV
SRMLNYMEQE VIGPDGIITV NDFTQNPRVQ LE
