ROP1B_HUMAN
ID ROP1B_HUMAN Reviewed; 212 AA.
AC Q9BZX4; D3DNA6; Q96BM7;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Ropporin-1B;
DE AltName: Full=Rhophilin-associated protein 1B;
GN Name=ROPN1B;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH AKAP3, AND
RP MUTAGENESIS OF LEU-18.
RC TISSUE=Testis;
RX PubMed=11278869; DOI=10.1074/jbc.m011252200;
RA Carr D.W., Fujita A., Stentz C.L., Liberty G.A., Olson G.E., Narumiya S.;
RT "Identification of sperm-specific proteins that interact with A-kinase
RT anchoring proteins in a manner similar to the type II regulatory subunit of
RT PKA.";
RL J. Biol. Chem. 276:17332-17338(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Important for male fertility. With ROPN1L, involved in
CC fibrous sheath integrity and sperm motility, plays a role in PKA-
CC dependent signaling processes required for spermatozoa capacitation.
CC {ECO:0000250|UniProtKB:Q9ESG2}.
CC -!- SUBUNIT: Homodimer. Interacts with RHPN1 (By similarity). May interact
CC with SPA17 (By similarity). Interacts with AKAP3 (PubMed:11278869).
CC Interacts with FSCB; the interaction increases upon spermatozoa
CC capacitation conditions (By similarity). {ECO:0000250|UniProtKB:Q9ESG2,
CC ECO:0000250|UniProtKB:Q9HAT0, ECO:0000269|PubMed:11278869}.
CC -!- INTERACTION:
CC Q9BZX4; O75969: AKAP3; NbExp=2; IntAct=EBI-9033148, EBI-9033101;
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum
CC {ECO:0000250|UniProtKB:Q9ESG2}. Note=In the sperm tail, found in the
CC principal piece and in the cytoplasmic droplet located at the distal
CC end of the midpiece. Inner surface of the fibrous sheath.
CC {ECO:0000250|UniProtKB:Q9ESG2}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9BZX4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9BZX4-2; Sequence=VSP_028745;
CC -!- DOMAIN: The RIIa domain mediates interaction with AKAP3.
CC -!- PTM: Sumoylated, sumoylation decreases upon spermatozoa capacitation
CC conditions. {ECO:0000250|UniProtKB:Q9ESG2}.
CC -!- MISCELLANEOUS: 'Ropporin' comes from the Japanese word 'oppo' which
CC means 'tail'.
CC -!- SIMILARITY: Belongs to the ropporin family. {ECO:0000305}.
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DR EMBL; AF231410; AAG59586.1; -; mRNA.
DR EMBL; CH471052; EAW79382.1; -; Genomic_DNA.
DR EMBL; CH471052; EAW79383.1; -; Genomic_DNA.
DR EMBL; BC015413; AAH15413.1; -; mRNA.
DR EMBL; BC141849; AAI41850.1; -; mRNA.
DR CCDS; CCDS33841.1; -. [Q9BZX4-1]
DR RefSeq; NP_001012337.1; NM_001012337.2. [Q9BZX4-1]
DR RefSeq; NP_001295242.1; NM_001308313.1. [Q9BZX4-1]
DR RefSeq; XP_005247195.1; XM_005247138.3. [Q9BZX4-2]
DR RefSeq; XP_006713576.1; XM_006713513.3. [Q9BZX4-1]
DR AlphaFoldDB; Q9BZX4; -.
DR SMR; Q9BZX4; -.
DR BioGRID; 127418; 1.
DR IntAct; Q9BZX4; 2.
DR STRING; 9606.ENSP00000426271; -.
DR PhosphoSitePlus; Q9BZX4; -.
DR BioMuta; ROPN1B; -.
DR DMDM; 74717806; -.
DR MassIVE; Q9BZX4; -.
DR PaxDb; Q9BZX4; -.
DR PeptideAtlas; Q9BZX4; -.
DR PRIDE; Q9BZX4; -.
DR ProteomicsDB; 79919; -. [Q9BZX4-1]
DR ProteomicsDB; 79920; -. [Q9BZX4-2]
DR Antibodypedia; 33037; 85 antibodies from 20 providers.
DR DNASU; 152015; -.
DR Ensembl; ENST00000251776.8; ENSP00000251776.4; ENSG00000114547.10. [Q9BZX4-1]
DR Ensembl; ENST00000505382.5; ENSP00000421662.1; ENSG00000114547.10. [Q9BZX4-2]
DR Ensembl; ENST00000511082.1; ENSP00000424447.1; ENSG00000114547.10. [Q9BZX4-2]
DR Ensembl; ENST00000514116.6; ENSP00000426271.1; ENSG00000114547.10. [Q9BZX4-1]
DR GeneID; 152015; -.
DR KEGG; hsa:152015; -.
DR MANE-Select; ENST00000514116.6; ENSP00000426271.1; NM_001308313.2; NP_001295242.1.
DR UCSC; uc003eih.4; human. [Q9BZX4-1]
DR CTD; 152015; -.
DR DisGeNET; 152015; -.
DR GeneCards; ROPN1B; -.
DR HGNC; HGNC:31927; ROPN1B.
DR HPA; ENSG00000114547; Tissue enriched (testis).
DR MIM; 611757; gene.
DR neXtProt; NX_Q9BZX4; -.
DR OpenTargets; ENSG00000114547; -.
DR PharmGKB; PA142671053; -.
DR VEuPathDB; HostDB:ENSG00000114547; -.
DR eggNOG; ENOG502R2JI; Eukaryota.
DR GeneTree; ENSGT00390000012731; -.
DR HOGENOM; CLU_069829_1_0_1; -.
DR InParanoid; Q9BZX4; -.
DR OMA; RAISNNG; -.
DR PhylomeDB; Q9BZX4; -.
DR TreeFam; TF105421; -.
DR PathwayCommons; Q9BZX4; -.
DR SignaLink; Q9BZX4; -.
DR BioGRID-ORCS; 152015; 16 hits in 1062 CRISPR screens.
DR GenomeRNAi; 152015; -.
DR Pharos; Q9BZX4; Tbio.
DR PRO; PR:Q9BZX4; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; Q9BZX4; protein.
DR Bgee; ENSG00000114547; Expressed in left testis and 94 other tissues.
DR ExpressionAtlas; Q9BZX4; baseline and differential.
DR Genevisible; Q9BZX4; HS.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0031514; C:motile cilium; IDA:UniProtKB.
DR GO; GO:0046982; F:protein heterodimerization activity; IPI:UniProtKB.
DR GO; GO:0030159; F:signaling receptor complex adaptor activity; NAS:UniProtKB.
DR GO; GO:0007340; P:acrosome reaction; NAS:UniProtKB.
DR GO; GO:0098609; P:cell-cell adhesion; NAS:UniProtKB.
DR GO; GO:0044782; P:cilium organization; ISS:UniProtKB.
DR GO; GO:0061640; P:cytoskeleton-dependent cytokinesis; NAS:UniProtKB.
DR GO; GO:0030317; P:flagellated sperm motility; ISS:UniProtKB.
DR GO; GO:0007342; P:fusion of sperm to egg plasma membrane involved in single fertilization; NAS:UniProtKB.
DR GO; GO:0061512; P:protein localization to cilium; ISS:UniProtKB.
DR GO; GO:0001932; P:regulation of protein phosphorylation; ISS:UniProtKB.
DR GO; GO:0007266; P:Rho protein signal transduction; TAS:UniProtKB.
DR GO; GO:0048240; P:sperm capacitation; ISS:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; NAS:UniProtKB.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell projection; Cilium; Flagellum; Phosphoprotein;
KW Reference proteome; Ubl conjugation.
FT CHAIN 1..212
FT /note="Ropporin-1B"
FT /id="PRO_0000307393"
FT DOMAIN 12..49
FT /note="RIIa"
FT REGION 209..212
FT /note="Interaction with RHPN1"
FT /evidence="ECO:0000250"
FT MOD_RES 56
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q4KLL5"
FT VAR_SEQ 1..92
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_028745"
FT MUTAGEN 18
FT /note="L->A: Abolishes interaction with AKAP3."
FT /evidence="ECO:0000269|PubMed:11278869"
SQ SEQUENCE 212 AA; 23964 MW; 2867946D20C637AE CRC64;
MAQTDKPTCI PPELPKMLKE FAKAAIRAQP QDLIQWGADY FEALSRGETP PVRERSERVA
LCNWAELTPE LLKILHSQVA GRLIIRAEEL AQMWKVVNLP TDLFNSVMNV GRFTEEIEWL
KFLALACSAL GVTITKTLKI VCEVLSCDHN GGLPRIPFST FQFLYTYIAE VDGEICASHV
SRMLNYIEQE VIGPDGLITV NDFTQNPRVW LE
