ROP1L_MOUSE
ID ROP1L_MOUSE Reviewed; 218 AA.
AC Q9EQ00; Q6P8Z9;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2017, sequence version 2.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Ropporin-1-like protein;
DE AltName: Full=AKAP-associated sperm protein;
GN Name=Ropn1l; Synonyms=Asp;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=BALB/cJ; TISSUE=Testis;
RX PubMed=12021058; DOI=10.1095/biolreprod66.6.1755;
RA Anway M.D., Ravindranath N., Dym M., Griswold M.D.;
RT "Identification of a murine testis complementary DNA encoding a homolog to
RT human A-kinase anchoring protein-associated sperm protein.";
RL Biol. Reprod. 66:1755-1761(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=11278869; DOI=10.1074/jbc.m011252200;
RA Carr D.W., Fujita A., Stentz C.L., Liberty G.A., Olson G.E., Narumiya S.;
RT "Identification of sperm-specific proteins that interact with A-kinase
RT anchoring proteins in a manner similar to the type II regulatory subunit of
RT PKA.";
RL J. Biol. Chem. 276:17332-17338(2001).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND SUBCELLULAR LOCATION.
RX PubMed=23303679; DOI=10.1095/biolreprod.112.105262;
RA Fiedler S.E., Dudiki T., Vijayaraghavan S., Carr D.W.;
RT "Loss of R2D2 proteins ROPN1 and ROPN1L causes defects in murine sperm
RT motility, phosphorylation, and fibrous sheath integrity.";
RL Biol. Reprod. 88:41-41(2013).
RN [6]
RP SUMOYLATION, AND INTERACTION WITH FSCB.
RX PubMed=27398160;
RA Zhang X., Chen M., Yu R., Liu B., Tian Z., Liu S.;
RT "FSCB phosphorylation regulates mouse spermatozoa capacitation through
RT suppressing SUMOylation of ROPN1/ROPN1L.";
RL Am. J. Transl. Res. 8:2776-2782(2016).
CC -!- FUNCTION: Important for male fertility. With ROPN1, involved in fibrous
CC sheath integrity and sperm motility, plays a role in PKA-dependent
CC signaling processes required for spermatozoa capacitation.
CC {ECO:0000269|PubMed:23303679}.
CC -!- SUBUNIT: May interact with AKAP3 (By similarity). Interacts with FSCB;
CC the interaction increases upon spermatozoa capacitation conditions
CC (PubMed:27398160). {ECO:0000250|UniProtKB:Q96C74,
CC ECO:0000269|PubMed:27398160}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum
CC {ECO:0000269|PubMed:23303679}. Cell projection, cilium
CC {ECO:0000250|UniProtKB:Q96C74}.
CC -!- TISSUE SPECIFICITY: Testis-specific. Expression is restricted to germ
CC cells. {ECO:0000269|PubMed:11278869, ECO:0000269|PubMed:12021058,
CC ECO:0000269|PubMed:23303679}.
CC -!- DEVELOPMENTAL STAGE: Expressed in testis at least from P5 to P40.
CC Expression increases with sample age. Detected in the flagella of
CC elongated spermatids, but the expression levels reduce as the sperm
CC matures (heads move towards the center lumen) (PubMed:23303679).
CC {ECO:0000269|PubMed:12021058, ECO:0000269|PubMed:23303679}.
CC -!- PTM: Sumoylated, sumoylation decreases upon spermatozoa capacitation
CC conditions. {ECO:0000269|PubMed:27398160}.
CC -!- DISRUPTION PHENOTYPE: Mutant mice have normal testicular morphology and
CC spermatogenesis but have moderately impaired motility and increased
CC levels of ROPN1 (PubMed:23303679). Double knockout animals for ROPN1
CC and ROPN1L are infertile with normal testicular morphology and
CC spermatogenesis but defects in sperm morphology, thinning and shredding
CC of the principal piece. Sperm is immotile (PubMed:23303679).
CC {ECO:0000269|PubMed:23303679}.
CC -!- SIMILARITY: Belongs to the ropporin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG40851.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF305427; AAG40851.1; ALT_FRAME; mRNA.
DR EMBL; BC048680; AAH48680.1; -; mRNA.
DR EMBL; BC060990; AAH60990.1; -; mRNA.
DR CCDS; CCDS37053.1; -.
DR RefSeq; NP_665851.2; NM_145852.2.
DR AlphaFoldDB; Q9EQ00; -.
DR STRING; 10090.ENSMUSP00000106038; -.
DR iPTMnet; Q9EQ00; -.
DR PhosphoSitePlus; Q9EQ00; -.
DR MaxQB; Q9EQ00; -.
DR PaxDb; Q9EQ00; -.
DR PeptideAtlas; Q9EQ00; -.
DR PRIDE; Q9EQ00; -.
DR ProteomicsDB; 260830; -.
DR Antibodypedia; 22503; 182 antibodies from 23 providers.
DR DNASU; 252967; -.
DR Ensembl; ENSMUST00000110408; ENSMUSP00000106038; ENSMUSG00000022236.
DR GeneID; 252967; -.
DR KEGG; mmu:252967; -.
DR CTD; 83853; -.
DR MGI; MGI:2182357; Ropn1l.
DR VEuPathDB; HostDB:ENSMUSG00000022236; -.
DR eggNOG; ENOG502QTNR; Eukaryota.
DR GeneTree; ENSGT00390000012731; -.
DR InParanoid; Q9EQ00; -.
DR OMA; DSRKNGM; -.
DR OrthoDB; 1316863at2759; -.
DR PhylomeDB; Q9EQ00; -.
DR TreeFam; TF105421; -.
DR BioGRID-ORCS; 252967; 1 hit in 73 CRISPR screens.
DR ChiTaRS; Ropn1l; mouse.
DR PRO; PR:Q9EQ00; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q9EQ00; protein.
DR Bgee; ENSMUSG00000022236; Expressed in seminiferous tubule of testis and 105 other tissues.
DR GO; GO:0005929; C:cilium; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0031514; C:motile cilium; IDA:MGI.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0003341; P:cilium movement; IMP:MGI.
DR GO; GO:0030317; P:flagellated sperm motility; IMP:MGI.
DR GO; GO:0001932; P:regulation of protein phosphorylation; IMP:MGI.
DR GO; GO:0048240; P:sperm capacitation; IMP:MGI.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Flagellum; Reference proteome; Ubl conjugation.
FT CHAIN 1..218
FT /note="Ropporin-1-like protein"
FT /id="PRO_0000307400"
FT DOMAIN 17..54
FT /note="RIIa"
SQ SEQUENCE 218 AA; 24516 MW; CB2C2F95FA032762 CRC64;
MPLPDTMFCA QQIHIPPELP DILKQFTKAA IRTQPADVLQ WSAGYFSALS RGDPLPVKDR
IEMPVATQKT DTGLTQGLLK VLHKQCSHKQ YVELADLEKK WKNLCLPVEK LRTILELDPC
EDKIEWIKFL ALGCSSLGRT LNTAMKNVCE ILTSDPEGGP ARIPFETFAY VYQYLSGLDP
ELPAVETENY LTSLRLMSES RKNGMIGLSD FFVGKKII
