ROP4_TOXGO
ID ROP4_TOXGO Reviewed; 578 AA.
AC Q5Y808; Q9U0D6;
DT 03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 25-MAY-2022, entry version 67.
DE RecName: Full=Rhoptry protein 4;
DE Flags: Precursor;
GN Name=ROP4 {ECO:0000312|EMBL:AAU87405.1}; ORFNames=TgIa.0490;
OS Toxoplasma gondii.
OC Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia;
OC Eucoccidiorida; Eimeriorina; Sarcocystidae; Toxoplasma.
OX NCBI_TaxID=5811;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAU87405.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], SUBCELLULAR LOCATION, AND
RP PHOSPHORYLATION.
RC STRAIN=RH {ECO:0000312|EMBL:AAU87405.1};
RX PubMed=15470260; DOI=10.1128/ec.3.5.1320-1330.2004;
RA Carey K.L., Jongco A.M., Kim K., Ward G.E.;
RT "The Toxoplasma gondii rhoptry protein rop4 is secreted into the
RT parasitophorous vacuole and becomes phosphorylated in infected cells.";
RL Eukaryot. Cell 3:1320-1330(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=RH;
RX PubMed=16902086; DOI=10.1101/gr.5318106;
RA Khan A., Bohme U., Kelly K.A., Adlem E., Brooks K., Simmonds M.,
RA Mungall K., Quail M.A., Arrowsmith C., Chillingworth T., Churcher C.,
RA Harris D., Collins M., Fosker N., Fraser A., Hance Z., Jagels K., Moule S.,
RA Murphy L., O'Neil S., Rajandream M.-A., Saunders D., Seeger K.,
RA Whitehead S., Mayr T., Xuan X., Watanabe J., Suzuki Y., Wakaguri H.,
RA Sugano S., Sugimoto C., Paulsen I., Mackey A.J., Roos D.S., Hall N.,
RA Berriman M., Barrell B., Sibley L.D., Ajioka J.W.;
RT "Common inheritance of chromosome Ia associated with clonal expansion of
RT Toxoplasma gondii.";
RL Genome Res. 16:1119-1125(2006).
RN [3] {ECO:0000305, ECO:0000312|EMBL:CAA96467.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=RH {ECO:0000312|EMBL:CAA96467.1};
RA Fourmaux M., Touzel J.;
RT "ROP 4 of Toxoplasma gondii.";
RL Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Thought to play a role in parasitophorous vacuole membrane
CC function during the infection of host organisms.
CC {ECO:0000269|PubMed:15470260}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15470260}.
CC Parasitophorous vacuole membrane {ECO:0000269|PubMed:15470260}.
CC Note=Localized to rhoptries which are secretory organelles at the
CC apical end of the parasite and secreted during host cell invasion when
CC it becomes associated with the parasitophorous vacuole membrane.
CC -!- PTM: Phosphorylated on multiple serine and threonine residues in
CC parasitic extracts and infected cells but not in extracellular
CC parasites. {ECO:0000269|PubMed:15470260}.
CC -!- CAUTION: Lacks the active site residue so is predicted to be
CC catalytically inactive. {ECO:0000269|PubMed:15470260}.
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DR EMBL; AY662677; AAU87405.1; -; Genomic_DNA.
DR EMBL; AM055942; CAJ20303.1; -; Genomic_DNA.
DR EMBL; Z71787; CAA96467.1; -; Genomic_DNA.
DR AlphaFoldDB; Q5Y808; -.
DR SMR; Q5Y808; -.
DR VEuPathDB; ToxoDB:TGARI_295110; -.
DR VEuPathDB; ToxoDB:TGCAST_295125; -.
DR VEuPathDB; ToxoDB:TGCAST_365010; -.
DR VEuPathDB; ToxoDB:TGCOUG_396160; -.
DR VEuPathDB; ToxoDB:TGDOM2_295110; -.
DR VEuPathDB; ToxoDB:TGFOU_295125; -.
DR VEuPathDB; ToxoDB:TGFOU_365080; -.
DR VEuPathDB; ToxoDB:TGGT1_295110; -.
DR VEuPathDB; ToxoDB:TGMAS_295125; -.
DR VEuPathDB; ToxoDB:TGMAS_417850; -.
DR VEuPathDB; ToxoDB:TGME49_295110; -.
DR VEuPathDB; ToxoDB:TGP89_421470; -.
DR VEuPathDB; ToxoDB:TGPRC2_295110; -.
DR VEuPathDB; ToxoDB:TGRH88_020200; -.
DR VEuPathDB; ToxoDB:TGRUB_365010; -.
DR VEuPathDB; ToxoDB:TGRUB_434430; -.
DR VEuPathDB; ToxoDB:TGVAND_295105; -.
DR VEuPathDB; ToxoDB:TGVAND_438930; -.
DR VEuPathDB; ToxoDB:TGVEG_295110; -.
DR Proteomes; UP000002437; Chromosome Ia.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0020008; C:rhoptry; IDA:UniProtKB.
DR GO; GO:0020005; C:symbiont-containing vacuole membrane; IDA:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0044409; P:entry into host; IEP:UniProtKB.
DR GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR InterPro; IPR027916; Kinase-like_dom_Apicomplexa.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR016815; Rhoptry_Rop2-like.
DR Pfam; PF14531; Kinase-like; 1.
DR PIRSF; PIRSF022995; Rhoptry_ROP2; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
PE 1: Evidence at protein level;
KW Membrane; Phosphoprotein; Reference proteome; Secreted; Signal.
FT SIGNAL 1..33
FT /evidence="ECO:0000255"
FT CHAIN 34..578
FT /note="Rhoptry protein 4"
FT /evidence="ECO:0000255"
FT /id="PRO_0000252103"
FT DOMAIN 259..546
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REGION 56..82
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 559..578
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 164..189
FT /note="AAGRLFGRVRQVEAERPQPVFTEGDP -> PLADCSGEFGKLRRNVHNPFSL
FT KVS (in Ref. 3; CAA96467)"
FT /evidence="ECO:0000305"
FT CONFLICT 556
FT /note="A -> R (in Ref. 3; CAA96467)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 578 AA; 64003 MW; AD1FF356820983C3 CRC64;
MGHPTSFGQP SCLVWLAAAF LVLGLCLVQQ GAGRQRPHQW KSSEAALSVS PAGDIVDKYS
RDSTEGENTV SEGEAEGSRG GSWLEQEGVE LRSPSQDSQT GTSTASPTGF RRLLRRLRFW
RRGSTRGSDD AAEVSRRTRV PLHTRLLQHL RRVARIIRHG VSAAAGRLFG RVRQVEAERP
QPVFTEGDPP DLETNSLYYR DKVPGQGIIQ EILRQKPGIA HHPESFSVVA ADERVSRTLW
AEGGVVRVAS ELGQPGRVLV RGRRIGLFRP GMQFEATDQA TGEPMTALVG HTVLEATARD
VDSMRNEGLA VGLFQKVKNP YLANRYLRFL APFDLVTIPG KPLVQKAKSR NEVGWVKNLL
FLLPPTHVDM ETFVDEIGRF PQEDRPLADA ARLYLTVQAV RLVAHLQDEG VVHGKIMPDS
FCLKREGGLY LRDFGSLVRA GAKVVVPAEY DEYTPPEGRA AARSRFGSGA TTMTYAFDAW
TLGSVIFLIW CSRAPDTKSG YEYSVEFFFS RCRRVPENVK LLVYKLINPS VEARLLALQA
IETPEYREME EQLSAASRLY SGDGTLTGGD DDMPPLET
