ROPE_PLAF7
ID ROPE_PLAF7 Reviewed; 1979 AA.
AC O96133; A0A144A0C5;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 25-MAY-2022, entry version 98.
DE RecName: Full=Repetitive organellar protein {ECO:0000250|UniProtKB:Q25662};
GN Name=ROPE {ECO:0000250|UniProtKB:Q25662}; ORFNames=PF3D7_0203000, PFB0145c;
OS Plasmodium falciparum (isolate 3D7).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC Plasmodiidae; Plasmodium; Plasmodium (Laverania).
OX NCBI_TaxID=36329;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3D7;
RX PubMed=9804551; DOI=10.1126/science.282.5391.1126;
RA Gardner M.J., Tettelin H., Carucci D.J., Cummings L.M., Aravind L.,
RA Koonin E.V., Shallom S.J., Mason T., Yu K., Fujii C., Pederson J., Shen K.,
RA Jing J., Aston C., Lai Z., Schwartz D.C., Pertea M., Salzberg S.L.,
RA Zhou L., Sutton G.G., Clayton R., White O., Smith H.O., Fraser C.M.,
RA Adams M.D., Venter J.C., Hoffman S.L.;
RT "Chromosome 2 sequence of the human malaria parasite Plasmodium
RT falciparum.";
RL Science 282:1126-1132(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3D7;
RX PubMed=12368864; DOI=10.1038/nature01097;
RA Gardner M.J., Hall N., Fung E., White O., Berriman M., Hyman R.W.,
RA Carlton J.M., Pain A., Nelson K.E., Bowman S., Paulsen I.T., James K.D.,
RA Eisen J.A., Rutherford K.M., Salzberg S.L., Craig A., Kyes S., Chan M.-S.,
RA Nene V., Shallom S.J., Suh B., Peterson J., Angiuoli S., Pertea M.,
RA Allen J., Selengut J., Haft D., Mather M.W., Vaidya A.B., Martin D.M.A.,
RA Fairlamb A.H., Fraunholz M.J., Roos D.S., Ralph S.A., McFadden G.I.,
RA Cummings L.M., Subramanian G.M., Mungall C., Venter J.C., Carucci D.J.,
RA Hoffman S.L., Newbold C., Davis R.W., Fraser C.M., Barrell B.G.;
RT "Genome sequence of the human malaria parasite Plasmodium falciparum.";
RL Nature 419:498-511(2002).
RN [3] {ECO:0000305}
RP SYNTHESIS OF 140-166; 249-273; 537-561; 575-621; 999-1020; 1126-1224 AND
RP 1252-1276, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND POSSIBLE
RP CANDIDATE MALARIA EPITOPE.
RX PubMed=17653272; DOI=10.1371/journal.pone.0000645;
RA Villard V., Agak G.W., Frank G., Jafarshad A., Servis C., Nebie I.,
RA Sirima S.B., Felger I., Arevalo-Herrera M., Herrera S., Heitz F.,
RA Baecker V., Druilhe P., Kajava A.V., Corradin G.;
RT "Rapid identification of malaria vaccine candidates based on alpha-helical
RT coiled coil protein motif.";
RL PLoS ONE 2:E645-E645(2007).
CC -!- SUBCELLULAR LOCATION: Host cell membrane {ECO:0000305|PubMed:17653272}.
CC Note=In schizonts and free merozoites, localizes to a punctate
CC structure in the cytoplasm which could correspond the rhoptry or
CC another apical organelle (By similarity). Localizes to the cell surface
CC of the host erythrocytes (Probable). {ECO:0000250|UniProtKB:Q25662,
CC ECO:0000305|PubMed:17653272}.
CC -!- DEVELOPMENTAL STAGE: Expressed during the asexual blood stage.
CC {ECO:0000269|PubMed:17653272}.
CC -!- BIOTECHNOLOGY: Possible candidate for an effective malaria vaccine as
CC determined by epitope response in sera. {ECO:0000269|PubMed:17653272}.
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DR EMBL; LN999943; CZT98043.1; -; Genomic_DNA.
DR PIR; C71622; C71622.
DR RefSeq; XP_001349543.1; XM_001349507.1.
DR AlphaFoldDB; O96133; -.
DR SMR; O96133; -.
DR BioGRID; 1207947; 1.
DR IntAct; O96133; 1.
DR STRING; 5833.PFB0145c; -.
DR PRIDE; O96133; -.
DR EnsemblProtists; CZT98043; CZT98043; PF3D7_0203000.
DR GeneID; 812625; -.
DR KEGG; pfa:PF3D7_0203000; -.
DR VEuPathDB; PlasmoDB:PF3D7_0203000; -.
DR HOGENOM; CLU_243476_0_0_1; -.
DR InParanoid; O96133; -.
DR OMA; NEHKINT; -.
DR PhylomeDB; O96133; -.
DR Proteomes; UP000001450; Chromosome 2.
DR GO; GO:0005737; C:cytoplasm; NAS:UniProtKB.
DR GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; NAS:UniProtKB.
PE 1: Evidence at protein level;
KW Coiled coil; Host cell membrane; Host membrane; Membrane; Merozoite;
KW Reference proteome.
FT CHAIN 1..1979
FT /note="Repetitive organellar protein"
FT /id="PRO_0000356832"
FT REGION 1..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 54..116
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 127..366
FT /evidence="ECO:0000255"
FT COILED 412..666
FT /evidence="ECO:0000255"
FT COILED 693..876
FT /evidence="ECO:0000255"
FT COILED 992..1094
FT /evidence="ECO:0000255"
FT COILED 1126..1307
FT /evidence="ECO:0000255"
FT COILED 1398..1467
FT /evidence="ECO:0000255"
FT COMPBIAS 65..116
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1979 AA; 237747 MW; 5C6CD6307AFF7D37 CRC64;
MVFTFKNKKK KKEASSDKVS KESFNEEDNE NNEKREKSDS WYKKIIETKG KSKTKYKNDN
SLDDNINEDI INNNNNNNND NNNDNNNDNN NDNNNDNNND NNNENNNDNN NFNNYSDEIS
KNIIHKDNEL ENQLKDTLKS ISSLSNKIVN YESKIEELEK ELKEVKDKNI DNNDYENKLK
EKEDFVKQKI DMLNEKENLL QEKELDINKR EKKINEKEKN IIKKEETFHN IEKEYLEKNK
ERETISIEII DIKKHLEKLK IEIKEKKEDL ENLNKKLLSK ENVLKELKGC VKEKNETINS
LNDNIIEKEK KYKLLEYELE EKNKQIDLLN KQEKEKEKEK EREKEKEREK EKEKEYDTLI
KELKDEKISI LEKVHSIKVR EMDIEKREHN FLHMEDQLKD LKNSFVKNNN QLKVYKCEIK
NLKTELEKKE KELKDIENVS KEEINKLINQ LNEKEKQILA FNKNHKEEIH GLKEELKESV
KITKIETQEL QEMVDIKQKE LDQLQEKYNA QIESISIELS KKEKEYNQYK NTYIEEINNL
NEKLEETNKE YTNLQNNYTN EINMLNNDIH MLNGNIKTMN TQISTLKNDV HLLNEQIDKL
NNEKGTLNSK ISELNVQIMD LKEEKDFLNN QIVDLSNQID LLTRKMEEKE NKMLEQENKY
KQEMELLRGN IKSSENILNN DEEVCDLKRK LSLKESEMKM MKEEHDKKLA ELKDDCDVRI
REMNEKNEDK INMLKEEYED KINTLKEQNE DKINTLKEQN EDKINTLKEE YEHKINTMKE
EYEHKINTLN EQNEHKINTL NEQNEHKINT MKEEYEDKMN TLNEQNEDKM NSLKEEYENK
INQINSNNEI KIKDVVNEYI EEVDKLKVTL DEKKKQFDKE INYAHIKAHE KEQILLTEME
ELKCQRDNKY SDLYEKYIKL IKSICMIINI ECCDDIENED IIRRIEEYIN NNKGLKKEVE
EKEHKRHSSF NILKSKEKFF KNSIEDKSHE LKKKHEKDLL SKDKEIEEKN KKIKELNNDI
KKLQDEILVY KKQSNAQQVD HKKKSWILLK DKSKEKIKDK ENQINVEKNE EKDLKKKDDE
IRILNEELVK YKTILYNLKK DPLLQNQDLL SKIDINSLTI NEGMCVDKIE EHILDYDEEI
NKSRSNLFQL KNEICSLTTE VMELNNKKNE LIEENNKLNL VDQGKKKLKK DVEKQKKEIE
KLNKQLTKCN KQIDELNEEV EKLNNENIEL ITYSNDLNNK FDMKENNLMM KLDENEDNIK
KMKSKIDDME KEIKYREDEK KRNLNEINNL KKKNEDMCIK YNEMNIKYGD ICVKYEEMSL
TYKETSLKYE QIKVKYDEKC SQYDEIRFQY DEKCFQYDEI NKKYGALLNI NITNKMVDSK
VDRNNNEIIS VDNKVEGIAN YLKQIFELNE EIIRLKGEIN KISLLYSNEL NEKNSYDINM
KHIQEQLLFL EKTNKENEEK IINLTSQYSD AYKKKSDESK LCGAQFVDDV NIYGNISNNN
IRTNEYKYEE MFDTNIEEKN GMHLSKYIHL LEENKFRCMK IIYENENIKS SNKIIGLYNY
SRYYGLREDL CKEEIVPSKI GNISNKNENN NKKNNTCDGY DEKVTIVLCI ILNEIIKFLF
LNDEYVLLFE KIHKNVWKRM YIPEEIKFFI LKYITLLNNL RDYIISVHNN MKNEKYDECW
FLFQHYFERS SDVRKEMVHF LLERKSQENL ISFKSKLKSK KEKILTMDIL NFSKEHMQLK
TIAHLRKEIN YEKLSKDTLN RDYNLLLYKY QECVSKLKRV KNLMKEINQN VFIEKYDDIS
KELDNFSDGY NEQNEQHVMD PILLNNNKNK NNKLITEHNN PIINRLTNFT QNRDSKYKNK
IMDDVKQRKI NSTMNNTNKN GINIIYNHYE NLNKPNYNDN INRLNSYHQN IHIANSIHPN
RNQNKSFLTN QANSTYSVMK NYINSDKPNL NGKKSVRNIF NEIVDENVNK TFVHKSVFF
