ROQT_PENRW
ID ROQT_PENRW Reviewed; 539 AA.
AC B6HJU0;
DT 08-JUN-2016, integrated into UniProtKB/Swiss-Prot.
DT 16-DEC-2008, sequence version 1.
DT 03-AUG-2022, entry version 69.
DE RecName: Full=Efflux pump roqT {ECO:0000305};
DE AltName: Full=Roquefortine/meleagrin synthesis protein T {ECO:0000303|PubMed:23776469};
GN Name=roqT {ECO:0000303|PubMed:23776469}; ORFNames=Pc21g15420;
OS Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin
OS 54-1255) (Penicillium chrysogenum).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium;
OC Penicillium chrysogenum species complex.
OX NCBI_TaxID=500485;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255;
RX PubMed=18820685; DOI=10.1038/nbt.1498;
RA van den Berg M.A., Albang R., Albermann K., Badger J.H., Daran J.-M.,
RA Driessen A.J.M., Garcia-Estrada C., Fedorova N.D., Harris D.M.,
RA Heijne W.H.M., Joardar V.S., Kiel J.A.K.W., Kovalchuk A., Martin J.F.,
RA Nierman W.C., Nijland J.G., Pronk J.T., Roubos J.A., van der Klei I.J.,
RA van Peij N.N.M.E., Veenhuis M., von Doehren H., Wagner C., Wortman J.R.,
RA Bovenberg R.A.L.;
RT "Genome sequencing and analysis of the filamentous fungus Penicillium
RT chrysogenum.";
RL Nat. Biotechnol. 26:1161-1168(2008).
RN [2]
RP FUNCTION.
RX PubMed=22118684; DOI=10.1016/j.chembiol.2011.08.012;
RA Garcia-Estrada C., Ullan R.V., Albillos S.M., Fernandez-Bodega M.A.,
RA Durek P., von Doehren H., Martin J.F.;
RT "A single cluster of coregulated genes encodes the biosynthesis of the
RT mycotoxins roquefortine C and meleagrin in Penicillium chrysogenum.";
RL Chem. Biol. 18:1499-1512(2011).
RN [3]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=23776469; DOI=10.1371/journal.pone.0065328;
RA Ali H., Ries M.I., Nijland J.G., Lankhorst P.P., Hankemeier T.,
RA Bovenberg R.A., Vreeken R.J., Driessen A.J.;
RT "A branched biosynthetic pathway is involved in production of roquefortine
RT and related compounds in Penicillium chrysogenum.";
RL PLoS ONE 8:E65328-E65328(2013).
RN [4]
RP BIOTECHNOLOGY.
RX PubMed=26692349; DOI=10.1016/j.bmc.2015.11.038;
RA Mady M.S., Mohyeldin M.M., Ebrahim H.Y., Elsayed H.E., Houssen W.E.,
RA Haggag E.G., Soliman R.F., El Sayed K.A.;
RT "The indole alkaloid meleagrin, from the olive tree endophytic fungus
RT Penicillium chrysogenum, as a novel lead for the control of c-Met-dependent
RT breast cancer proliferation, migration and invasion.";
RL Bioorg. Med. Chem. 24:113-122(2016).
CC -!- FUNCTION: Efflux pump; part of the gene cluster that mediates the
CC biosynthesis of the mycotoxins roquefortine C and meleagrin
CC (PubMed:22118684). {ECO:0000269|PubMed:22118684}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: Expression is decreased in presence of phenylacetic acid
CC (PAA) (PubMed:23776469). {ECO:0000269|PubMed:23776469}.
CC -!- DISRUPTION PHENOTYPE: Does not affect the biosynthesis of roquefortine
CC C and meleagrin (PubMed:23776469). {ECO:0000269|PubMed:23776469}.
CC -!- BIOTECHNOLOGY: The indole alkaloid meleagrin was shown to be a good
CC candidate to control c-Met-dependent breast cancer proliferation,
CC migration and invasion (PubMed:26692349).
CC {ECO:0000269|PubMed:26692349}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. TCR/Tet
CC family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AM920436; CAP96439.1; -; Genomic_DNA.
DR RefSeq; XP_002568552.1; XM_002568506.1.
DR AlphaFoldDB; B6HJU0; -.
DR SMR; B6HJU0; -.
DR STRING; 1108849.XP_002568552.1; -.
DR EnsemblFungi; CAP96439; CAP96439; PCH_Pc21g15420.
DR GeneID; 8315543; -.
DR KEGG; pcs:Pc21g15420; -.
DR VEuPathDB; FungiDB:PCH_Pc21g15420; -.
DR eggNOG; KOG0254; Eukaryota.
DR HOGENOM; CLU_000960_22_1_1; -.
DR OMA; WALNAYN; -.
DR OrthoDB; 627633at2759; -.
DR BioCyc; PCHR:PC21G15420-MON; -.
DR Proteomes; UP000000724; Contig Pc00c21.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00217; SUGAR_TRANSPORT_2; 1.
PE 1: Evidence at protein level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..539
FT /note="Efflux pump roqT"
FT /id="PRO_0000436349"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 108..128
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 160..180
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 191..211
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 233..253
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 262..282
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 305..325
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 338..360
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 362..384
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 395..415
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 502..522
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 539 AA; 58050 MW; FFE36D70F196D7A0 CRC64;
MEKEVATDPL PQEIPSDAPD EGGSLSRPAA NTWTVVSLTI ALCLGVFCMS LDVTIITTAI
PRITDQFDSL DDIGWYGSSY LLTNCATTLA FGKFYTFYST KWVYLSALFL FEVGSLVCGV
TPTSVGLILG RCIAGLGAGG LFSGSLLIIA QTVPLHRRPV FTALLGSMYG IASVAGPPLG
GALTDRVSWR WCFYINLPIG AVTAAFVLFF FHAPNSVKRR PELRKLLSEL DPIGSFFFLP
AIVCLLLALQ WGGTQYSWKS PRIIVLFVLT GVLLLAFVAV QIRQNEKATL PPRIVQNRNI
WSSAWFAITL NGAYFVFIYY LPIWFQAIKA ASATKSGVMN LPSIIAVVVV SIISGMLVTI
FGYYNPVMIM SSVTLSIGAG LLSTLKTDSG SGEWIGYQIL MGLGVGLGMQ QPFMVVQNVL
PDGDVPTGTA VITFAQTLGG AIFISVGQNI FQNQFAHTMH LEDPSVNVAT VLSAGTTTLR
KYLPAEQLPA VLRSYNTAIT QAFYVGVALA SLSCIGTIAL EWKSVKKPRN TTPNDHSGS
