ROS3_ARATH
ID ROS3_ARATH Reviewed; 748 AA.
AC Q9FGT1; Q8L734;
DT 11-DEC-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Protein REPRESSOR OF SILENCING 3 {ECO:0000303|PubMed:18815596};
GN Name=ROS3 {ECO:0000303|PubMed:18815596};
GN OrderedLocusNames=At5g58130 {ECO:0000312|Araport:AT5G58130};
GN ORFNames=K21L19.13 {ECO:0000312|EMBL:BAB11005.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP AND DOMAIN.
RC STRAIN=cv. C24;
RX PubMed=18815596; DOI=10.1038/nature07305;
RA Zheng X., Pontes O., Zhu J., Miki D., Zhang F., Li W.-X., Iida K.,
RA Kapoor A., Pikaard C.S., Zhu J.-K.;
RT "ROS3 is an RNA-binding protein required for DNA demethylation in
RT Arabidopsis.";
RL Nature 455:1259-1262(2008).
CC -!- FUNCTION: RNA-binding protein required for DNA demethylation and to
CC eluviate siRNA-mediated transcriptional gene silencing (TGS), probably
CC by guiding ROS1 (PubMed:18815596). Can bind specifically single
CC stranded G-rich RNAs of 21-, 24- or 26-nt corresponding to promoter
CC sequence of target genes; this interaction directs demethylation of
CC target sequences (PubMed:18815596). {ECO:0000269|PubMed:18815596}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:18815596}. Nucleus,
CC nucleolus {ECO:0000269|PubMed:18815596}. Nucleus, nucleoplasm
CC {ECO:0000269|PubMed:18815596}. Note=Localized in discrete foci
CC dispersed throughout the nucleus. {ECO:0000269|PubMed:18815596}.
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed.
CC {ECO:0000269|PubMed:18815596}.
CC -!- DOMAIN: The RRM domain is required for single stranded RNA binding.
CC {ECO:0000269|PubMed:18815596}.
CC -!- DISRUPTION PHENOTYPE: DNA hypermethylation (at CpG, CpNpG and CpNpN
CC sites, N is A, T or C) leading to increased siRNA-mediated
CC transcriptional gene silencing (TGS) (PubMed:18815596). Narrow and
CC slightly lobed second and third pairs of true leaves (PubMed:18815596).
CC Disrupted ROS1 subcellular localization in the nucleolus
CC (PubMed:18815596). {ECO:0000269|PubMed:18815596}.
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DR EMBL; AB024029; BAB11005.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97000.1; -; Genomic_DNA.
DR EMBL; AY139982; AAM98125.1; -; mRNA.
DR EMBL; AK229306; BAF01169.1; -; mRNA.
DR RefSeq; NP_200621.1; NM_125198.5.
DR AlphaFoldDB; Q9FGT1; -.
DR SMR; Q9FGT1; -.
DR STRING; 3702.AT5G58130.1; -.
DR PaxDb; Q9FGT1; -.
DR PRIDE; Q9FGT1; -.
DR ProteomicsDB; 175271; -.
DR EnsemblPlants; AT5G58130.1; AT5G58130.1; AT5G58130.
DR GeneID; 835925; -.
DR Gramene; AT5G58130.1; AT5G58130.1; AT5G58130.
DR KEGG; ath:AT5G58130; -.
DR Araport; AT5G58130; -.
DR TAIR; locus:2155811; AT5G58130.
DR eggNOG; ENOG502QPQA; Eukaryota.
DR HOGENOM; CLU_021819_1_0_1; -.
DR InParanoid; Q9FGT1; -.
DR OMA; DTAIDSM; -.
DR OrthoDB; 280693at2759; -.
DR PhylomeDB; Q9FGT1; -.
DR PRO; PR:Q9FGT1; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FGT1; baseline and differential.
DR GO; GO:0005730; C:nucleolus; IDA:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003723; F:RNA binding; IDA:TAIR.
DR GO; GO:0003727; F:single-stranded RNA binding; IDA:UniProtKB.
DR GO; GO:0080111; P:DNA demethylation; IMP:TAIR.
DR GO; GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
DR GO; GO:1901535; P:regulation of DNA demethylation; IMP:UniProtKB.
DR GO; GO:0060966; P:regulation of gene silencing by RNA; IMP:UniProtKB.
DR CDD; cd12226; RRM_NOL8; 1.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR034138; NOP8_RRM.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 1.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 2: Evidence at transcript level;
KW Nucleus; Reference proteome; RNA-binding; RNA-mediated gene silencing.
FT CHAIN 1..748
FT /note="Protein REPRESSOR OF SILENCING 3"
FT /id="PRO_0000448735"
FT DOMAIN 10..86
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 244..318
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 350..531
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 579..600
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 729..748
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 244..261
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 267..284
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 285..313
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 439..453
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 493..516
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 404
FT /note="T -> A (in Ref. 3; AAM98125)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 748 AA; 81080 MW; 629B9A44CBBC6B29 CRC64;
MEEKSSGGGV RLHVGGLGES VGRDDLLKIF SPMGTVDAVE FVRTKGRSFA YIDFSPSSTN
SLTKLFSTYN GCVWKGGRLR LEKAKEHYLA RLKREWEAAS STSDNTIKAP SDSPPATHLN
IFFPRLRKVK PMPLSGTGKH KYSFQRVPVS SSLPRSFCDC EEHSNSSLTP REIHLHDLEA
VNVGRQEAEV NVMNSVMNKL FEKNNVDPEE DNEIEADQDN LIINVASSGN DMDSALDMLS
RKRKSILNKK TPSEEGYSEG RKGNLTHPSK NRQTISLEET GRQESSQAIR GKKKPSEVVP
DKSSDEPSRT KDLEQSIDNI SWSQKSSWKS LMANGNSNDF SVSSFLPGVG SSKAVQPAPR
NTDLAGLPSR ENLKKKTKRK RVTSTIMAED LPVSDDIKRD DSDTMADDIE RDDSDAVEYY
TACESMADDT ASDSVAERDD SDAVEDDTAI DSMADDPASD SVAESDDGDA VENDTAIDSM
ADDTVSNSMA ESDDGDNVED DTAIDSMCDD TANDDVGSDD SGSLADTVSD TSVEAVPLEF
VANTEGDSVD GKSNVEKHEN VAEDLNAEKE SLVVKENVVD EEEAGKGPLK ASNKSTGGSS
WLQKASWTQL VSDKNTSSFS ITQLFPDLTS DKGEAAGVIN NVGNQFSNSN QTASAMKQTD
YASSSGGFVA AGVPVDSTPV RSLDENRQRL NGKNVSEGAK LGAKKKIIKR KVGSGDTCTF
MRSSTSLKEW AKAKKALSEP RRKKNSEE
