ROST_DROME
ID ROST_DROME Reviewed; 275 AA.
AC O44252; Q5U0W2; Q95RK6; Q9VLF9;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Protein rolling stone;
GN Name=rost; ORFNames=CG9552;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RC STRAIN=Canton-S; TISSUE=Embryo, and Mesoderm;
RX PubMed=9230076; DOI=10.1083/jcb.138.2.337;
RA Paululat A., Goubeaud A., Damm C., Knirr S., Burchard S.,
RA Renkawitz-Pohl R.;
RT "The mesodermal expression of rolling stone (rost) is essential for
RT myoblast fusion in Drosophila and encodes a potential transmembrane
RT protein.";
RL J. Cell Biol. 138:337-348(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 6-275.
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: May have a central role in the fusion process during
CC myogenesis, within the somatic mesoderm. {ECO:0000269|PubMed:9230076}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:9230076}; Multi-pass
CC membrane protein {ECO:0000269|PubMed:9230076}. Note=Enriched in the
CC embryonic membrane fraction.
CC -!- TISSUE SPECIFICITY: Expressed in cells of the somatic mesoderm, most
CC notably the muscle founder cells, between embryonic stages 12 and 14,
CC in growing muscle fibers in dorsal, lateral and ventral positions. At
CC stage 16 strongest expression is in some ventral muscles and muscle 8.
CC At stages 16/17 expression is restricted to some cells of the CNS, the
CC brain and the gonads. {ECO:0000269|PubMed:9230076}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically. High
CC level of zygotic expression is seen in embryos from stage 12 onwards
CC and in adults. {ECO:0000269|PubMed:9230076}.
CC -!- DISRUPTION PHENOTYPE: Flies exhibit diminished fusion of embryonic
CC myoblasts to syncytial myotubes. {ECO:0000269|PubMed:9230076}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL28858.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF006955; AAB96894.1; -; mRNA.
DR EMBL; AE014134; AAF52733.1; -; Genomic_DNA.
DR EMBL; BT016130; AAV37015.1; -; mRNA.
DR EMBL; AY061310; AAL28858.1; ALT_INIT; mRNA.
DR RefSeq; NP_001260274.1; NM_001273345.1.
DR RefSeq; NP_001260275.1; NM_001273346.1.
DR RefSeq; NP_723435.1; NM_164842.2.
DR AlphaFoldDB; O44252; -.
DR STRING; 7227.FBpp0303870; -.
DR PaxDb; O44252; -.
DR PRIDE; O44252; -.
DR DNASU; 34222; -.
DR EnsemblMetazoa; FBtr0079782; FBpp0079382; FBgn0011705.
DR EnsemblMetazoa; FBtr0331453; FBpp0303870; FBgn0011705.
DR EnsemblMetazoa; FBtr0331454; FBpp0303871; FBgn0011705.
DR GeneID; 34222; -.
DR KEGG; dme:Dmel_CG9552; -.
DR CTD; 34222; -.
DR FlyBase; FBgn0011705; rost.
DR VEuPathDB; VectorBase:FBgn0011705; -.
DR eggNOG; ENOG502S8CX; Eukaryota.
DR GeneTree; ENSGT00730000112105; -.
DR HOGENOM; CLU_066320_1_0_1; -.
DR InParanoid; O44252; -.
DR OMA; HLFYRSQ; -.
DR OrthoDB; 1191165at2759; -.
DR PhylomeDB; O44252; -.
DR BioGRID-ORCS; 34222; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 34222; -.
DR PRO; PR:O44252; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0011705; Expressed in thoracico-abdominal ganglion (Drosophila) and 25 other tissues.
DR ExpressionAtlas; O44252; baseline and differential.
DR Genevisible; O44252; DM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IDA:FlyBase.
DR GO; GO:0007520; P:myoblast fusion; IMP:FlyBase.
DR InterPro; IPR039860; Rost.
DR PANTHER; PTHR12242:SF1; PTHR12242:SF1; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Membrane; Muscle protein; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..275
FT /note="Protein rolling stone"
FT /id="PRO_0000097405"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 72..92
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 127..147
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 162..182
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 185..205
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 232..252
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 120
FT /note="R -> T (in Ref. 1; AAB96894)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 275 AA; 32635 MW; E166C66459B15C96 CRC64;
MQLFDDFCKS FNKELQRANF GFAYNRVHLF YRSQWQKDEI NTIYLLYRWI WALFFLGVYI
MCVIVQFCDG KFFIYMTNWG FGLCTITMLI SAVQVTCWHF DVRSTRSLVQ ESGHKAETSR
GLKIYWWLYN MTLSLALIIS TVYWVFLHGK MNKPMRFPAI SIITHGMNSV MMLIDFLVIA
FPLRILHMVY GMSLAIFFFL FTLIYHLCGG TDEFGNHYVY PILDWNNPNR CMVTFVGIFL
LIMCYWVLLF GLYKLKRMFN RAFSVVWSPH AVGLI
