ID   ROT1_LITRO              Reviewed;          11 AA.
AC   P86508;
DT   15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT   15-JUN-2010, sequence version 1.
DT   11-DEC-2019, entry version 6.
DE   RecName: Full=Rothein 1 {ECO:0000303|PubMed:16124032};
OS   Litoria rothii (Roth's tree frog) (Hyla rothii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Pelodryadinae; Litoria.
OX   NCBI_TaxID=336074;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP   MASS SPECTROMETRY.
RC   TISSUE=Skin secretion {ECO:0000269|PubMed:16124032};
RX   PubMed=16124032; DOI=10.1002/rcm.2098;
RA   Brinkworth C.S., Bowie J.H., Bilusich D., Tyler M.J.;
RT   "The rothein peptides from the skin secretion of Roth's tree frog Litoria
RT   rothii. Sequence determination using positive and negative ion electrospray
RT   mass spectrometry.";
RL   Rapid Commun. Mass Spectrom. 19:2716-2724(2005).
RN   [2] {ECO:0000305}
RP   FUNCTION, STRUCTURE BY NMR OF 1-11, AND MUTAGENESIS OF SER-1; SER-3; GLU-7;
RP   SER-8 AND PHE-11.
RX   PubMed=19539637; DOI=10.1016/j.toxicon.2009.06.009;
RA   Sherman P.J., Jackway R.J., Nicholson E., Musgrave I.F., Boontheung P.,
RA   Bowie J.H.;
RT   "Activities of seasonably variable caerulein and rothein skin peptides from
RT   the tree frogs Litoria splendida and Litoria rothii.";
RL   Toxicon 54:828-835(2009).
CC   -!- FUNCTION: Induces contraction of intestinal smooth muscle in isolated
CC       guinea pig ileum. Induces proliferation of mouse splenocytes.
CC       {ECO:0000269|PubMed:16124032, ECO:0000269|PubMed:19539637}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16124032}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin dorsal glands.
CC       {ECO:0000269|PubMed:16124032}.
CC   -!- MASS SPECTROMETRY: Mass=1149; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:16124032};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Rothein subfamily. {ECO:0000305}.
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DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0070665; P:positive regulation of leukocyte proliferation; IDA:UniProtKB.
DR   GO; GO:0045987; P:positive regulation of smooth muscle contraction; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Direct protein sequencing; Secreted.
FT   PEPTIDE         1..11
FT                   /note="Rothein 1"
FT                   /evidence="ECO:0000269|PubMed:16124032"
FT                   /id="PRO_0000394434"
FT   MUTAGEN         1
FT                   /note="S->A: Increases mouse splenocyte proliferation."
FT                   /evidence="ECO:0000269|PubMed:19539637"
FT   MUTAGEN         3
FT                   /note="S->A: Increases mouse splenocyte proliferation."
FT                   /evidence="ECO:0000269|PubMed:19539637"
FT   MUTAGEN         7
FT                   /note="E->A: Abolishes mouse splenocyte proliferation."
FT                   /evidence="ECO:0000269|PubMed:19539637"
FT   MUTAGEN         8
FT                   /note="S->A: Abolishes mouse splenocyte proliferation."
FT                   /evidence="ECO:0000269|PubMed:19539637"
FT   MUTAGEN         11
FT                   /note="F->A: Abolishes mouse splenocyte proliferation."
FT                   /evidence="ECO:0000269|PubMed:19539637"
SQ   SEQUENCE   11 AA;  1149 MW;  1955B89ABB076054 CRC64;
     SVSNIPESIG F