ID   ROT22_LITRO             Reviewed;          22 AA.
AC   P86510;
DT   15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT   15-JUN-2010, sequence version 1.
DT   25-MAY-2022, entry version 6.
DE   RecName: Full=Rothein 2.2 {ECO:0000303|PubMed:16124032};
OS   Litoria rothii (Roth's tree frog) (Hyla rothii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Pelodryadinae; Litoria.
OX   NCBI_TaxID=336074;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS
RP   SPECTROMETRY, AND AMIDATION AT LEU-22.
RC   TISSUE=Skin secretion {ECO:0000269|PubMed:16124032};
RX   PubMed=16124032; DOI=10.1002/rcm.2098;
RA   Brinkworth C.S., Bowie J.H., Bilusich D., Tyler M.J.;
RT   "The rothein peptides from the skin secretion of Roth's tree frog Litoria
RT   rothii. Sequence determination using positive and negative ion electrospray
RT   mass spectrometry.";
RL   Rapid Commun. Mass Spectrom. 19:2716-2724(2005).
CC   -!- FUNCTION: Lacks antimicrobial activity. Does not inhibit the formation
CC       of NO by neuronal nitric oxide. {ECO:0000269|PubMed:16124032}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16124032}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin dorsal glands.
CC       {ECO:0000269|PubMed:16124032}.
CC   -!- MASS SPECTROMETRY: Mass=2281; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:16124032};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Rothein subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P86510; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Amphibian defense peptide; Direct protein sequencing; Secreted.
FT   PEPTIDE         1..22
FT                   /note="Rothein 2.2"
FT                   /evidence="ECO:0000269|PubMed:16124032"
FT                   /id="PRO_0000394436"
FT   MOD_RES         22
FT                   /note="Leucine amide"
FT                   /evidence="ECO:0000269|PubMed:16124032"
SQ   SEQUENCE   22 AA;  2284 MW;  6B9D16EC0E07E4F5 CRC64;
     AGDDETLLKP VLNSLDNLVS GL