ID   ROT_STAA8               Reviewed;         166 AA.
AC   Q9RFJ6; Q2FXG8;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   25-JUL-2006, sequence version 2.
DT   25-MAY-2022, entry version 107.
DE   RecName: Full=HTH-type transcriptional regulator rot;
DE   AltName: Full=Repressor of toxins;
GN   Name=rot; OrderedLocusNames=SAOUHSC_01879;
OS   Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX   PubMed=10809700; DOI=10.1128/jb.182.11.3197-3203.2000;
RA   McNamara P.J., Milligan-Monroe K.C., Khalili S., Proctor R.A.;
RT   "Identification, cloning, and initial characterization of rot, a locus
RT   encoding a regulator of virulence factor expression in Staphylococcus
RT   aureus.";
RL   J. Bacteriol. 182:3197-3203(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 8325 / PS 47;
RA   Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT   "The Staphylococcus aureus NCTC 8325 genome.";
RL   (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL   Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL   D.C. (2006).
RN   [3]
RP   FUNCTION.
RX   PubMed=12511508; DOI=10.1128/jb.185.2.610-619.2003;
RA   Said-Salim B., Dunman P.M., McAleese F.M., Macapagal D., Murphy E.,
RA   McNamara P.J., Arvidson S., Foster T.J., Projan S.J., Kreiswirth B.N.;
RT   "Global regulation of Staphylococcus aureus genes by Rot.";
RL   J. Bacteriol. 185:610-619(2003).
RN   [4]
RP   FUNCTION, AND REGULATION BY AGR.
RX   PubMed=14996810; DOI=10.1128/jb.186.6.1793-1801.2004;
RA   Tseng C.W., Zhang S., Stewart G.C.;
RT   "Accessory gene regulator control of staphyloccoccal enterotoxin D gene
RT   expression.";
RL   J. Bacteriol. 186:1793-1801(2004).
CC   -!- FUNCTION: Global regulator with both positive and negative effects that
CC       mediates modulation of several genes involved in virulence. Negatively
CC       regulates the transcription of several known virulence factors such as
CC       lipase (geh), hemolysins (hla and hlb) and proteases (splA through
CC       splF, sspB and sspC). Positively regulates the expression of sarS and
CC       other genes including those encoding cell surface adhesins (clfB, sdrC
CC       and spa). Also, modulates the expression of genes not previously
CC       implicated in pathogenesis. {ECO:0000269|PubMed:10809700,
CC       ECO:0000269|PubMed:12511508, ECO:0000269|PubMed:14996810}.
CC   -!- MISCELLANEOUS: Virulence accessory gene regulator (Agr) and Rot have
CC       opposing effects on the expression of selected target genes and agr
CC       also controls negatively rot activity by an unknown mechanism.
CC   -!- SIMILARITY: Belongs to the rot family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABD30944.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AF189239; AAF22306.1; -; Genomic_DNA.
DR   EMBL; CP000253; ABD30944.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; YP_500382.1; NC_007795.1.
DR   AlphaFoldDB; Q9RFJ6; -.
DR   SMR; Q9RFJ6; -.
DR   STRING; 1280.SAXN108_1793; -.
DR   EnsemblBacteria; ABD30944; ABD30944; SAOUHSC_01879.
DR   GeneID; 3921768; -.
DR   KEGG; sao:SAOUHSC_01879; -.
DR   PATRIC; fig|93061.5.peg.1711; -.
DR   eggNOG; COG1846; Bacteria.
DR   HOGENOM; CLU_132118_0_0_9; -.
DR   Proteomes; UP000008816; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR000835; HTH_MarR-typ.
DR   InterPro; IPR016998; Rot.
DR   InterPro; IPR010166; SarA/Rot.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF01047; MarR; 1.
DR   PIRSF; PIRSF032474; TF_HTH_Rot; 1.
DR   SMART; SM00347; HTH_MARR; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   TIGRFAMs; TIGR01889; Staph_reg_Sar; 1.
PE   3: Inferred from homology;
KW   Activator; DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation; Virulence.
FT   CHAIN           1..166
FT                   /note="HTH-type transcriptional regulator rot"
FT                   /id="PRO_0000220543"
FT   DNA_BIND        87..110
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        84
FT                   /note="S -> F (in Ref. 1; AAF22306)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   166 AA;  19371 MW;  D01AFF4E304E732C CRC64;
     MHKLAHTSFG IVGMFVNTCI VAKYVIINWE MFSMKKVNND TVFGILQLET LLGDINSIFS
     EIESEYKMSR EEILILLTLW QKGSMTLKEM DRFVEVKPYK RTRTYNNLVE LEWIYKERPV
     DDERTVIIHF NEKLQQEKVE LLNFISDAIA SRATAMQNSL NAIIAV