ID   ROT_STAAR               Reviewed;         166 AA.
AC   Q6GFT9;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 95.
DE   RecName: Full=HTH-type transcriptional regulator rot;
DE   AltName: Full=Repressor of toxins;
GN   Name=rot; OrderedLocusNames=SAR1847;
OS   Staphylococcus aureus (strain MRSA252).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=282458;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MRSA252;
RX   PubMed=15213324; DOI=10.1073/pnas.0402521101;
RA   Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J.,
RA   Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A.,
RA   Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C.,
RA   Clark L., Corton C., Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z.,
RA   Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N.,
RA   Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A.,
RA   Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M.,
RA   Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J.;
RT   "Complete genomes of two clinical Staphylococcus aureus strains: evidence
RT   for the rapid evolution of virulence and drug resistance.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004).
CC   -!- FUNCTION: Global regulator with both positive and negative effects that
CC       mediates modulation of several genes involved in virulence. Also,
CC       modulates the expression of genes not previously implicated in
CC       pathogenesis (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the rot family. {ECO:0000305}.
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DR   EMBL; BX571856; CAG40838.1; -; Genomic_DNA.
DR   PDB; 4Q77; X-ray; 1.77 A; A/B=34-166.
DR   PDBsum; 4Q77; -.
DR   AlphaFoldDB; Q6GFT9; -.
DR   SMR; Q6GFT9; -.
DR   KEGG; sar:SAR1847; -.
DR   HOGENOM; CLU_132118_0_0_9; -.
DR   OMA; LWDKGSM; -.
DR   PRO; PR:Q6GFT9; -.
DR   Proteomes; UP000000596; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR000835; HTH_MarR-typ.
DR   InterPro; IPR016998; Rot.
DR   InterPro; IPR010166; SarA/Rot.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF01047; MarR; 1.
DR   PIRSF; PIRSF032474; TF_HTH_Rot; 1.
DR   SMART; SM00347; HTH_MARR; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   TIGRFAMs; TIGR01889; Staph_reg_Sar; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Activator; DNA-binding; Repressor; Transcription;
KW   Transcription regulation; Virulence.
FT   CHAIN           1..166
FT                   /note="HTH-type transcriptional regulator rot"
FT                   /id="PRO_0000220547"
FT   DNA_BIND        87..110
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   HELIX           41..66
FT                   /evidence="ECO:0007829|PDB:4Q77"
FT   HELIX           70..82
FT                   /evidence="ECO:0007829|PDB:4Q77"
FT   STRAND          84..86
FT                   /evidence="ECO:0007829|PDB:4Q77"
FT   HELIX           87..93
FT                   /evidence="ECO:0007829|PDB:4Q77"
FT   HELIX           98..100
FT                   /evidence="ECO:0007829|PDB:4Q77"
FT   HELIX           101..110
FT                   /evidence="ECO:0007829|PDB:4Q77"
FT   STRAND          113..117
FT                   /evidence="ECO:0007829|PDB:4Q77"
FT   STRAND          127..130
FT                   /evidence="ECO:0007829|PDB:4Q77"
FT   HELIX           132..134
FT                   /evidence="ECO:0007829|PDB:4Q77"
FT   HELIX           135..164
FT                   /evidence="ECO:0007829|PDB:4Q77"
SQ   SEQUENCE   166 AA;  19417 MW;  71CF1F6D5B90571D CRC64;
     MHKLAHISFG IVGMFVNTCM VAKYVIINWE MYSMKKVNND TVFGILQLET LLGDINSIFS
     EIESEYKMSR EEILILLTLW QKGSMTLKEM DRFVEVKPYK RTRTYNNLVE LEWIYKERPV
     DDERTVIIHF NEKLQQEKVE LLNFISDAIA SRATAMQNSL NAIIAV