ROUGH_DROME
ID ROUGH_DROME Reviewed; 350 AA.
AC P10181; Q24487; Q4V714; Q9VBA7;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 4.
DT 03-AUG-2022, entry version 184.
DE RecName: Full=Homeobox protein rough;
GN Name=ro; ORFNames=CG6348;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=2903798; DOI=10.1016/0092-8674(88)90133-x;
RA Tomlinson A., Kimmel B.E., Rubin G.M.;
RT "Rough, a Drosophila homeobox gene required in photoreceptors R2 and R5 for
RT inductive interactions in the developing eye.";
RL Cell 55:771-784(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 182-258, AND TISSUE SPECIFICITY.
RC STRAIN=Canton-S;
RX PubMed=2898735; DOI=10.1038/334151a0;
RA Saint R., Kalionis B., Lockett T.J., Elizur A.;
RT "Pattern formation in the developing eye of Drosophila melanogaster is
RT regulated by the homoeo-box gene, rough.";
RL Nature 334:151-154(1988).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=1974228; DOI=10.1101/gad.4.5.712;
RA Kimmel B.E., Heberlein U., Rubin G.M.;
RT "The homeo domain protein rough is expressed in a subset of cells in the
RT developing Drosophila eye where it can specify photoreceptor cell
RT subtype.";
RL Genes Dev. 4:712-727(1990).
RN [7]
RP FUNCTION.
RX PubMed=11709152; DOI=10.1016/s0896-6273(01)00480-9;
RA Frankfort B.J., Nolo R., Zhang Z., Bellen H., Mardon G.;
RT "senseless repression of rough is required for R8 photoreceptor
RT differentiation in the developing Drosophila eye.";
RL Neuron 32:403-414(2001).
CC -!- FUNCTION: Required to establish the unique cell identity of
CC photoreceptors R2 and R5 and consequently for ommatidial assembly in
CC the developing eye imaginal disk. Repression of expression in R8
CC photoreceptor by senseless (sens) is an essential mechanism of R8 cell
CC fate determination. {ECO:0000269|PubMed:11709152,
CC ECO:0000269|PubMed:1974228, ECO:0000269|PubMed:2903798}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC ECO:0000269|PubMed:1974228}. Note=Restricted to nuclei in the
CC developing retina.
CC -!- TISSUE SPECIFICITY: Expressed in cells within, and posterior to, the
CC morphogenetic furrow of the eye imaginal disk, expression is rapidly
CC restricted to the R2, R3, R4, and R5 precursor cells.
CC {ECO:0000269|PubMed:1974228, ECO:0000269|PubMed:2898735}.
CC -!- DISRUPTION PHENOTYPE: R2 and R5 fail to be correctly determined and
CC appear to be transformed into cells of the R3/4/1/6 subtype.
CC {ECO:0000269|PubMed:2903798}.
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DR EMBL; M23629; AAA56800.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF56635.1; -; Genomic_DNA.
DR EMBL; AE014297; AAN14093.1; -; Genomic_DNA.
DR EMBL; BT022142; AAY51537.1; -; mRNA.
DR EMBL; X12528; CAC32035.1; -; Genomic_DNA.
DR PIR; A30046; A30046.
DR PIR; S03168; S03168.
DR RefSeq; NP_524521.1; NM_079797.3.
DR RefSeq; NP_733173.1; NM_170294.1.
DR AlphaFoldDB; P10181; -.
DR SMR; P10181; -.
DR BioGRID; 68125; 18.
DR IntAct; P10181; 8.
DR STRING; 7227.FBpp0084461; -.
DR PaxDb; P10181; -.
DR PRIDE; P10181; -.
DR EnsemblMetazoa; FBtr0085090; FBpp0084460; FBgn0003267.
DR EnsemblMetazoa; FBtr0085091; FBpp0084461; FBgn0003267.
DR GeneID; 43234; -.
DR KEGG; dme:Dmel_CG6348; -.
DR UCSC; CG6348-RA; d. melanogaster.
DR UCSC; CG6348-RB; d. melanogaster.
DR CTD; 19875; -.
DR FlyBase; FBgn0003267; ro.
DR VEuPathDB; VectorBase:FBgn0003267; -.
DR eggNOG; KOG0489; Eukaryota.
DR GeneTree; ENSGT00940000172642; -.
DR HOGENOM; CLU_792903_0_0_1; -.
DR InParanoid; P10181; -.
DR OMA; CDTPYHS; -.
DR OrthoDB; 1583482at2759; -.
DR PhylomeDB; P10181; -.
DR BioGRID-ORCS; 43234; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 43234; -.
DR PRO; PR:P10181; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0003267; Expressed in embryonic/larval posterior spiracle (Drosophila) and 4 other tissues.
DR Genevisible; P10181; DM.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0042067; P:establishment of ommatidial planar polarity; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:FlyBase.
DR GO; GO:0048054; P:R2/R5 cell differentiation; IMP:FlyBase.
DR GO; GO:0007463; P:R2/R5 cell fate commitment; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0050896; P:response to stimulus; IEA:UniProtKB-KW.
DR GO; GO:0007601; P:visual perception; IEA:UniProtKB-KW.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR InterPro; IPR046327; HXA1/B1/D1.
DR PANTHER; PTHR45946; PTHR45946; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW Sensory transduction; Transcription; Transcription regulation; Vision.
FT CHAIN 1..350
FT /note="Homeobox protein rough"
FT /id="PRO_0000049076"
FT DNA_BIND 191..250
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 70..91
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 111..137
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 324..350
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 121..135
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 304
FT /note="A -> T (in Ref. 1; AAA56800)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 350 AA; 39430 MW; D5A39EF19D39DEAF CRC64;
MQRHKVEIGS PDGSPGIKRS DSLDPIANTT ILSVPQRPSS PRQFFERLYG HLETRSSENG
EIDVGTHAHK PPPCDTPYHS DGGSVSSPDI SISDERTSLA AYPAYDFYGH AKDYPQHPSQ
QHQQHHHHHH HPPQLVHQKL SYVSPPPAIA AGGAANPVLP HAFPAGFPSD PHFSAGFSAF
LARRRRKEGR QRRQRTTFST EQTLRLEVEF HRNEYISRSR RFELAETLRL TETQIKIWFQ
NRRAKDKRIE KAQIDQHYRN FVVANGFMSS IMGQAATTMP PGGVTGGVAV GVGLNYYAAA
ATPAALPKDN TQDANFIDID DQFQRQQQQK QQQQQQQQRR RETTTPINIC
