ID   RP132_FOWPN             Reviewed;        1161 AA.
AC   Q9J544;
DT   29-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=DNA-directed RNA polymerase 132 kDa polypeptide;
DE            EC=2.7.7.6;
GN   Name=RPO132; OrderedLocusNames=FPV189;
OS   Fowlpox virus (strain NVSL) (FPV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Avipoxvirus.
OX   NCBI_TaxID=928301;
OH   NCBI_TaxID=7742; Vertebrata.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=10729156; DOI=10.1128/jvi.74.8.3815-3831.2000;
RA   Afonso C.L., Tulman E.R., Lu Z., Zsak L., Kutish G.F., Rock D.L.;
RT   "The genome of fowlpox virus.";
RL   J. Virol. 74:3815-3831(2000).
CC   -!- FUNCTION: Part of the DNA-dependent RNA polymerase which catalyzes the
CC       transcription of viral DNA into RNA using the four ribonucleoside
CC       triphosphates as substrates. Responsible for the transcription of
CC       early, intermediate and late genes. DNA-dependent RNA polymerase
CC       associates with the early transcription factor (ETF), itself composed
CC       of D6 and A7, thereby allowing the early genes transcription. Late
CC       transcription, and probably also intermediate transcription, require
CC       newly synthesized RNA polymerase (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: The DNA-dependent RNA polymerase used for intermediate and
CC       late genes expression consists of eight subunits (147) kDa, (133) kDa,
CC       (35) kDa, (30) kDa, (22) kDa, (19) kDa, (18) kDa and (7) kDa totalling
CC       more than 500 kDa in mass. The same holoenzyme, with the addition of
CC       the transcription-specificity factor RAP94, is used for early gene
CC       expression (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250}. Note=All the enzymes and
CC       other proteins required to synthesize early mRNAs are packaged within
CC       the virion core along with the DNA genome. This is necessary because
CC       viral early mRNAs are synthesized within minutes after virus entry into
CC       the cell and are extruded through pores in the core particle (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000305}.
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DR   EMBL; AF198100; AAF44533.1; -; Genomic_DNA.
DR   RefSeq; NP_039152.1; NC_002188.1.
DR   SMR; Q9J544; -.
DR   GeneID; 1486761; -.
DR   KEGG; vg:1486761; -.
DR   Proteomes; UP000008597; Genome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR024390; RNA_pol_132_poxvirus.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   Pfam; PF12415; rpo132; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Metal-binding; Nucleotidyltransferase;
KW   Reference proteome; Transcription; Transferase; Virion.
FT   CHAIN           1..1161
FT                   /note="DNA-directed RNA polymerase 132 kDa polypeptide"
FT                   /id="PRO_0000048067"
SQ   SEQUENCE   1161 AA;  133469 MW;  334C2FC5DD63584F CRC64;
     MDQKLGNKFL EPDPKQNVFY RPLHFQYVSY ENFISYRLKE ILSVNRTLLS FKNDTEKIVL
     RINNIKIIPP DYSPIIASIK GKSYDALVTF TVDIRKEVMT KDGLHVSTIS SYEGNDSQLI
     KIPLLIGYGN KNPLDNSKFV SPNIIGGVFI NKQSIEKVGI NIVEKTTTWP KFKIVKPNAY
     TFSFSSISPV NILPTKYRHY KITMDLSQLE NCYISSAKTF ITVNVIVLIK FLINQDLNYI
     KNNLTYGMPL ETIYLINAII ESSKTILEAE DFNINDYIES LIESEFQKQR SITSIDDFRY
     DLMYNFLPHM VNSSDQLKGF YLLGLLRKFI YCIYHTSRYP DRDSMVCHRV LTYGRYFEIL
     ANDELENYIT NIKNDITNSH KNKGVCNVSI HVLTTPGFNH AFSGLLSGKF KKTDGSYRTH
     PHYSWMQNIS IPRSVGYYPD QVKISKMFSV RKYHPSQYAF FCPSDVPERG PQVGLISQLS
     VLTSVSNIRT TEYIDLKNAI MKYIYTYDKN DISYFQTGHI ITIENDLVAA INPELVDKFV
     DDFKFRKRVN YFDNLEIGIS NVKDHMNEIR INIGSGRLIR PFLVVYKGEL VMDTIGEELE
     KRIDTITFSD IQKEYPHVIE MLDLEQFVFS NVCESVSKFR ELSDEDKKLY DYCDFPNEFR
     DGYVASTLVG INHNSGPRAI LGCAQAKQAI SCLSSDLRNK IDNGIHLLYP ERPIVLSKAT
     ETSKIAINCF GQHVLVALMS YKGMNQEDGI VVKREFIERG GLDIVTAKKH QVEIPIENFK
     NRERINSTAY SKLDINGLVR LNAFLEPGDA IAKNISSRTL DDDFVADNQI SFDISEKYTD
     MYMSRVERVQ VDLTDKVKVR VLTMKERRPI MGDKFTSRTS QKGTIAYIAS ESELPYDKNG
     VTPDIIINST SIYSRKTISM LIEMILTSAY SVKPYNNNGK NRPICFPSSN ETDIEYYIEF
     ARKCYQSAIP DLDKDELENE VYCESILYDP ETDKPYKTKV FMGPLYYLRL RHLTQDKATV
     RCRGKKTKLI RQANEGRKRG GGIKFGEMER DCLIAHGAAN TITEILKDSE EDYQDVYVCE
     NCGDIATKKN NNVYCIRCTK LNLYTVLTKI DTTHVSKVFL TQMNARGIKI NLTFNEQNPL
     FYKPMKQIDL SPTILKNHDL S