ID   RP132_VACCA             Reviewed;        1156 AA.
AC   O57230;
DT   07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=DNA-directed RNA polymerase 133 kDa polypeptide;
DE            EC=2.7.7.6;
GN   Name=RPO132; OrderedLocusNames=MVA135R, ACAM3000_MVA_135;
OS   Vaccinia virus (strain Ankara) (VACV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=126794;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=9601507; DOI=10.1006/viro.1998.9123;
RA   Antoine G., Scheiflinger F., Dorner F., Falkner F.G.;
RT   "The complete genomic sequence of the modified vaccinia Ankara strain:
RT   comparison with other orthopoxviruses.";
RL   Virology 244:365-396(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Isolate Acambis 3000;
RA   Esposito J.J., Frace M., Sammons S.A., Olsen-Rasmussen M.S., Osborne J.,
RA   Khristova M., Wohlhueter R.M.;
RL   Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   REVIEW.
RX   PubMed=12917449; DOI=10.1099/vir.0.18942-0;
RA   Broyles S.S.;
RT   "Vaccinia virus transcription.";
RL   J. Gen. Virol. 84:2293-2303(2003).
CC   -!- FUNCTION: Part of the DNA-dependent RNA polymerase which catalyzes the
CC       transcription of viral DNA into RNA using the four ribonucleoside
CC       triphosphates as substrates. Responsible for the transcription of
CC       early, intermediate and late genes. DNA-dependent RNA polymerase
CC       associates with the early transcription factor (ETF), itself composed
CC       of D6 and A7, thereby allowing the early genes transcription. Late
CC       transcription, and probably also intermediate transcription, require
CC       newly synthesized RNA polymerase.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: The DNA-dependent RNA polymerase used for intermediate and
CC       late genes expression consists of eight subunits 147 kDa, 133 kDa, 35
CC       kDa, 30 kDa, 22 kDa, 19 kDa, 18 kDa and 7 kDa totalling more than 500
CC       kDa in mass. The same holoenzyme, with the addition of the
CC       transcription-specificity factor RAP94, is used for early gene
CC       expression.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Note=All the enzymes and
CC       other proteins required to synthesize early mRNAs are packaged within
CC       the virion core along with the DNA genome. This is necessary because
CC       viral early mRNAs are synthesized within minutes after virus entry into
CC       the cell and are extruded through pores in the core particle.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000305}.
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DR   EMBL; U94848; AAB96526.1; -; Genomic_DNA.
DR   EMBL; AY603355; AAT10534.1; -; Genomic_DNA.
DR   PIR; T37411; T37411.
DR   SMR; O57230; -.
DR   Proteomes; UP000159908; Genome.
DR   Proteomes; UP000172909; Genome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR024390; RNA_pol_132_poxvirus.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007647; RNA_pol_Rpb2_5.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF04567; RNA_pol_Rpb2_5; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   Pfam; PF12415; rpo132; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Metal-binding; Nucleotidyltransferase;
KW   Transcription; Transferase; Virion.
FT   CHAIN           1..1156
FT                   /note="DNA-directed RNA polymerase 133 kDa polypeptide"
FT                   /id="PRO_0000048058"
SQ   SEQUENCE   1156 AA;  132426 MW;  F6657C8AF5E22EC3 CRC64;
     MDQRLGYKFL VPDPKAGVFY RPLHFQYVSY SNFILHRLHE ILTVKRPLLS FKNNTERIMI
     EISNVKVTPP DYSPIIASIK GKSYDALATF TVNIFKEVMT KEGISITKIS SYEGKDSHLI
     KIPLLIGYGN KNPLDTAKYL VPNVIGGVFI NKQSVEKVGI NLVEKITTWP KFRVVKPNSF
     TFSFSSVSPP NVLPTRYRHY KISLDISQLE ALNISSTKTF ITVNIVLLSQ YLSRVSLEFI
     RRSLSYDMPP EVVYLVNAII DSAKRITESI TDFNIDTYIN DLVEAEHIKQ KSQLTINEFK
     YEMLHNFLPH MNYTPDQLKG FYMISLLRKF LYCIYHTSRY PDRDSMVCHR ILTYGKYFET
     LAHDELENYI GNIRNDIMNN HKNRGTYAVN IHVLTTPGLN HAFSSLLSGK FKKSDGSYRT
     HPHYSWMQNI SIPRSVGFYP DQVKISKMFS VRKYHPSQYL YFCSSDVPER GPQVGLVSQL
     SVLSSITNIL TSEYLDLEKK ICEYIRSYYK DDISYFETGF PITIENALVA SLNPNMICDF
     VTDFRRRKRM GFFGNLEVGI TLVRDHMNEI RINIGAGRLV RPFLVVDNGE LMMDVCPELE
     SRLDDMTFSD IQKEFPHVIE MVDIEQFTFS NVCESVQKFR MMSKDERKQY DLCDFPAEFR
     DGYVASSLVG INHNSGPRAI LGCAQAKQAI SCLSSDIRNK IDNGIHLMYP ERPIVISKAL
     ETSKIAANCF GQHVTIALMS YKGINQEDGI IIKKQFIQRG GLDIVTAKKH QVEIPLENFN
     NKERDRSNAY SKLESNGLVR LNAFLESGDA IARNISSRTL EDDFARDNQI SFDVSEKYTD
     MYKSRVERVQ VELTDKVKVR VLTMKERRPI LGDKFTTRTS QKGTVAYIAD ETELPYDENG
     ITPDVIINST SIFSRKTISM LIEVILTAAY SAKPYNNKGE NRPVCFPSSN ETSIDTYMQF
     AKQCYEHSNP KLSDEELSDK IFCEKILYDP ETDKPYASKV FFGPIYYLRL RHLTQDKATV
     RCRGKKTKLI RQANEGRKRG GGIKFGEMER DCLIAHGAAN TITEVLKDSE EDYQDVYVCE
     NCGDIAAQIK GINTCLRCSK LNLSPLLTKI DTTHVSKVFL TQMNARGVKV KLDFERRPPS
     FYKPLDKVDL KPSFLV