RP132_VACCW
ID RP132_VACCW Reviewed; 1164 AA.
AC Q76ZP7; P19798;
DT 07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=DNA-directed RNA polymerase 133 kDa polypeptide;
DE EC=2.7.7.6;
GN Name=RPO132; OrderedLocusNames=VACWR144; ORFNames=A24R;
OS Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS WR)).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX NCBI_TaxID=10254;
OH NCBI_TaxID=9913; Bos taurus (Bovine).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1824607; DOI=10.1016/0042-6822(91)90012-z;
RA Amegadzie B.Y., Holmes M.H., Cole N.B., Jones E.V., Earl P.L., Moss B.;
RT "Identification, sequence, and expression of the gene encoding the second-
RT largest subunit of the vaccinia virus DNA-dependent RNA polymerase.";
RL Virology 180:88-98(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA Wohlhueter R.;
RT "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT redundancy and an error rate of 0.16/10kb.";
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=16509968; DOI=10.1186/1743-422x-3-10;
RA Yoder J.D., Chen T.S., Gagnier C.R., Vemulapalli S., Maier C.S.,
RA Hruby D.E.;
RT "Pox proteomics: mass spectrometry analysis and identification of Vaccinia
RT virion proteins.";
RL Virol. J. 3:10-10(2006).
RN [4]
RP REVIEW.
RX PubMed=12917449; DOI=10.1099/vir.0.18942-0;
RA Broyles S.S.;
RT "Vaccinia virus transcription.";
RL J. Gen. Virol. 84:2293-2303(2003).
CC -!- FUNCTION: Part of the DNA-dependent RNA polymerase which catalyzes the
CC transcription of viral DNA into RNA using the four ribonucleoside
CC triphosphates as substrates. Responsible for the transcription of
CC early, intermediate and late genes. DNA-dependent RNA polymerase
CC associates with the early transcription factor (ETF), itself composed
CC of D6 and A7, thereby allowing the early genes transcription. Late
CC transcription, and probably also intermediate transcription, require
CC newly synthesized RNA polymerase.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6;
CC -!- SUBUNIT: The DNA-dependent RNA polymerase used for intermediate and
CC late genes expression consists of eight subunits 147 kDa, 133 kDa, 35
CC kDa, 30 kDa, 22 kDa, 19 kDa, 18 kDa and 7 kDa totalling more than 500
CC kDa in mass. The same holoenzyme, with the addition of the
CC transcription-specificity factor RAP94, is used for early gene
CC expression.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Note=All the enzymes and
CC other proteins required to synthesize early mRNAs are packaged within
CC the virion core along with the DNA genome. This is necessary because
CC viral early mRNAs are synthesized within minutes after virus entry into
CC the cell and are extruded through pores in the core particle.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000305}.
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DR EMBL; M37415; AAA72882.1; -; Genomic_DNA.
DR EMBL; AY243312; AAO89423.1; -; Genomic_DNA.
DR PIR; H42519; RNVZ8T.
DR RefSeq; YP_233026.1; NC_006998.1.
DR PDB; 6RIE; EM; 3.10 A; B=1-1164.
DR PDBsum; 6RIE; -.
DR SMR; Q76ZP7; -.
DR DNASU; 3707674; -.
DR GeneID; 3707674; -.
DR KEGG; vg:3707674; -.
DR Proteomes; UP000000344; Genome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR024390; RNA_pol_132_poxvirus.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007647; RNA_pol_Rpb2_5.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF04567; RNA_pol_Rpb2_5; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR Pfam; PF12415; rpo132; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Reference proteome; Transcription; Transferase;
KW Virion.
FT CHAIN 1..1164
FT /note="DNA-directed RNA polymerase 133 kDa polypeptide"
FT /id="PRO_0000048061"
FT HELIX 9..18
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 22..25
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 26..29
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 32..41
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 42..44
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 45..50
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 55..59
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 60..63
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 64..76
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 82..87
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 93..106
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 113..121
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 128..131
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 164..167
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 169..171
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 179..184
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 187..195
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 200..202
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 205..215
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 225..228
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 233..241
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 245..252
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 258..275
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 277..280
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 283..297
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 304..312
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 313..316
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 317..319
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 324..343
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 353..356
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 362..391
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 400..402
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 407..412
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 413..417
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 420..422
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 426..428
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 436..439
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 440..442
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 443..445
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 464..466
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 480..484
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 485..488
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 499..515
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 518..520
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 525..532
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 535..540
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 542..544
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 546..556
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 557..561
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 567..571
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 573..575
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 577..581
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 587..595
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 600..603
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 605..609
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 610..613
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 616..622
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 626..631
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 632..636
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 640..642
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 644..648
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 652..656
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 659..661
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 665..668
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 673..675
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 679..681
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 684..694
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 705..707
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 712..717
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 722..724
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 727..730
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 731..735
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 740..747
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 750..756
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 757..761
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 762..766
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 767..770
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 772..783
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 784..787
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 805..807
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 818..820
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 852..875
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 882..884
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 891..897
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 899..901
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 912..915
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 918..922
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 927..940
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 947..949
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 962..976
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 982..989
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 998..1000
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 1008..1019
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 1027..1031
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 1036..1038
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 1045..1048
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 1051..1053
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 1055..1064
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 1067..1074
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 1084..1086
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 1088..1090
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 1095..1102
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 1104..1108
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 1116..1118
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 1122..1132
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 1133..1135
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 1136..1143
FT /evidence="ECO:0007829|PDB:6RIE"
SQ SEQUENCE 1164 AA; 133364 MW; B1F5C1484BA37D0D CRC64;
MKKNTDSEMD QRLGYKFLVP DPKAGVFYRP LHFQYVSYSN FILHRLHEIL TVKRPLLSFK
NNTERIMIEI SNVKVTPPDY SPIIASIKGK SYDALATFTV NIFKEVMTKE GISITKISSY
EGKDSHLIKI PLLIGYGNKN PLDTAKYLVP NVIGGVFINK QSVEKVGINL VEKITTWPKF
RVVKPNSFTF SFSSVSPPNV LPTRYRHYKI SLDISQLEAL NISSTKTFIT VNIVLLSQYL
SRVSLEFIRR SLSYDMPPEV VYLVNAIIDS AKRITESITD FNIDTYINDL VEAEHIKQKS
QLTINEFKYE MLHNFLPHMN YTPDQLKGFY MISLLRKFLY CIYHTSRYPD RDSMVCHRIL
TYGKYFETLA HDELENYIGN IRNDIMNNHK NRGTYAVNIH VLTTPGLNHA FSSLLSGKFK
KSDGSYRTHP HYSWMQNISI PRSVGFYPDQ VKISKMFSVR KYHPSQYLYF CSSDVPERGP
QVGLVSQLSV LSSITNILTS EYLDLEKKIC EYIRSYYKDD ISYFETGFPI TIENALVASL
NPNMICDFVT DFRRRKRMGF FGNLEVGITL VRDHMNEIRI NIGAGRLVRP FLVVDNGELM
MDVCPELESR LDDMTFSDIQ KEFPHVIEMV DIEQFTFSNV CESVQKFRMM SKDERKQYDL
CDFPAEFRDG YVASSLVGIN HNSGPRAILG CAQAKQAISC LSSDIRNKID NGIHLMYPER
PIVISKALET SKIAANCFGQ HVTIALMSYK GINQEDGIII KKQFIQRGGL DIVTAKKHQV
EIPLENFNNK ERDRSNAYSK LESNGLVRLN AFLESGDAMA RNISSRTLED DFARDNQISF
DVSEKYTDMY KSRVERVQVE LTDKVKVRVL TMKERRPILG DKFTTRTSQK GTVAYVADET
ELPYDENGIT PDVIINSTSI FSRKTISMLI EVILTAAYSA KPYNNKGENR PVCFPSSNET
SIDTYMQFAK QCYEHSNPKL SDEELSDKIF CEKILYDPET DKPYASKVFF GPIYYLRLRH
LTQDKATVRC RGKKTKLIRQ ANEGRKRGGG IKFGEMERDC LIAHGAANTI TEVLKDSEED
YQDVYVCENC GDIAAQIKGI NTCLRCSKLN LSPLLTKIDT THVSKVFLTQ MNARGVKVKL
DFERRPPSFY KPLDKVDLKP SFLV
