ID   RP147_FOWPN             Reviewed;        1287 AA.
AC   Q9J593;
DT   29-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=DNA-directed RNA polymerase 147 kDa polypeptide;
DE            EC=2.7.7.6;
GN   Name=RPO147; OrderedLocusNames=FPV137;
OS   Fowlpox virus (strain NVSL) (FPV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Avipoxvirus.
OX   NCBI_TaxID=928301;
OH   NCBI_TaxID=7742; Vertebrata.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=10729156; DOI=10.1128/jvi.74.8.3815-3831.2000;
RA   Afonso C.L., Tulman E.R., Lu Z., Zsak L., Kutish G.F., Rock D.L.;
RT   "The genome of fowlpox virus.";
RL   J. Virol. 74:3815-3831(2000).
CC   -!- FUNCTION: Part of the DNA-dependent RNA polymerase which catalyzes the
CC       transcription of viral DNA into RNA using the four ribonucleoside
CC       triphosphates as substrates. Responsible for the transcription of
CC       early, intermediate and late genes. DNA-dependent RNA polymerase
CC       associates with the early transcription factor (ETF) thereby allowing
CC       the early genes transcription. Late transcription, and probably also
CC       intermediate transcription, require newly synthesized RNA polymerase
CC       (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: The DNA-dependent RNA polymerase used for intermediate and
CC       late genes expression consists of eight subunits (147) kDa, (133) kDa,
CC       (35) kDa, (30) kDa, (22) kDa, (19) kDa, (18) kDa and (7) kDa totalling
CC       more than 500 kDa in mass. The same holoenzyme, with the addition of
CC       the transcription-specificity factor RAP94, is used for early gene
CC       expression (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Note=All the enzymes and
CC       other proteins required to synthesize early mRNAs are packaged within
CC       the virion core along with the DNA genome. This is necessary because
CC       viral early mRNAs are synthesized within minutes after virus entry into
CC       the cell and are extruded through pores in the core particle (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae DNA-directed RNA polymerase 147
CC       kDa subunit family. {ECO:0000305}.
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DR   EMBL; AF198100; AAF44481.1; -; Genomic_DNA.
DR   RefSeq; NP_039100.1; NC_002188.1.
DR   SMR; Q9J593; -.
DR   GeneID; 1486685; -.
DR   KEGG; vg:1486685; -.
DR   Proteomes; UP000008597; Genome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.132.30; -; 1.
DR   Gene3D; 1.10.274.100; -; 1.
DR   Gene3D; 4.10.860.120; -; 1.
DR   InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR   InterPro; IPR000722; RNA_pol_asu.
DR   InterPro; IPR006592; RNA_pol_N.
DR   InterPro; IPR007066; RNA_pol_Rpb1_3.
DR   InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR   InterPro; IPR007083; RNA_pol_Rpb1_4.
DR   InterPro; IPR007081; RNA_pol_Rpb1_5.
DR   InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR   InterPro; IPR038120; Rpb1_funnel_sf.
DR   PANTHER; PTHR19376; PTHR19376; 1.
DR   Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR   Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR   Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR   Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR   SMART; SM00663; RPOLA_N; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase; Virion.
FT   CHAIN           1..1287
FT                   /note="DNA-directed RNA polymerase 147 kDa polypeptide"
FT                   /id="PRO_0000073922"
SQ   SEQUENCE   1287 AA;  147998 MW;  B9DC191393999E62 CRC64;
     MSVISKVSYS LYSQNEINAT DININYVKDD DEVGTVKDSR LGATDGVLCR TCNRTELECF
     GHWGKVRIYE NIIIKPEYIS EVIRILGHIC LTCGLLRSRE PYTVNISSLT SGELKKLKDK
     ISSKKKSCWN SRCMQPYQKV NFSKKKVCLV NKTDEFCVPN ALVYEKITSI HHKFWPVLDI
     HQDPATLFYR GYFSIPPLLI RPVISFWIDN VPKDTNELTY LLGVIVKHCN ANADEPTIQK
     AIIEYDNIKL ISTNSTTNNL SYITSGKTNM LRSFVVARRK DQTARSVLGP DSSLDITEVG
     IPDYVRNTLT EKIFINAFTI DKVKDMFQRG EIKYYFNKQL HQLTKIKQNK FIKNKIHLLP
     GDWVETNIQE FTNIIFGRQP SLHRYNVISS SVRKTEEDTI KIPPGIANSQ NADFDGDEEW
     TIVEQNPKSV IEQSILMYPT TLLKHDVHGM PVYGSIQDEI LAAYNLFREY DLTQDEVLNI
     LGKYGLEFLT DYERKDKYTG KDIFKFLINE PEINYPGIIC NGEIIAENID SNFIVSMKHM
     SISGLITDYK SSVEGIKFIN KASYVFKRYL KIYGFSITFR NLCPDFEFTK KLREQNIKKI
     NDIKHSYVKY LYDVANGDII PLSRSDEMDA VDSILSGLTN FNIQEIEKYM KEVISKDPDN
     SLMKMSCAGY KVNPTELMYI LGTYGQQRID GEPIDTKIYG RVLPYFLPDS KDPEGKGYIL
     NSLIQGLTGS QYYYAMLIAR SQSTDIVCET SRTGTLARKI IKKMEDMVVD SYGQIVYGNT
     LVKYAANYTK IQGSVCKSVE LIYPDESLTW FLEISALWDK LKNGFIYNQG QKIAKYILAP
     FNFKVFMKLD ETNPMKSKDL YDKIQLVIKD VRENYFFDVT SIDFIEYVFL THLNPSRVKV
     SEDTANLIFE KLYEKLNYTL GGGTPIGIIS AQVLSEKFTQ QALSSFHTTE KSGGIKRKLG
     FNEFNQLTNL SKNKTEIITL ISDDITKLQT IKMNFEFVYL GELFPEITIE EDKNYYRIDI
     NVNRLYIKRN QLTELIVEYM LEKFVSYSVL VKNWGMETNI INEHIIRFSL FIVFTEPVNL
     NKNKFMMMLP GAANKGKISK YKIPISEYQS YTDYNKTVKL YRLTVELMGL KELGTFDLVN
     VNVIPGVWNT YEIFGIESAK SYLCEALLST YGEGLDYLYQ PCDLLASLIC LNYEPESINK
     FKFGPVSALK RATFGDNKAI INAALYKKTE PVNDNSSCHF FSKVPKIGTG YYKYFIDLEK
     FLRIKKTISE KLIDKKLVDI GDNITDF