RP147_VACCA
ID RP147_VACCA Reviewed; 1286 AA.
AC O57204;
DT 07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=DNA-directed RNA polymerase 147 kDa polypeptide;
DE EC=2.7.7.6;
GN Name=RPO147; OrderedLocusNames=MVA090R, ACAM3000_MVA_090;
OS Vaccinia virus (strain Ankara) (VACV).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX NCBI_TaxID=126794;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=9601507; DOI=10.1006/viro.1998.9123;
RA Antoine G., Scheiflinger F., Dorner F., Falkner F.G.;
RT "The complete genomic sequence of the modified vaccinia Ankara strain:
RT comparison with other orthopoxviruses.";
RL Virology 244:365-396(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Isolate Acambis 3000;
RA Esposito J.J., Frace M., Sammons S.A., Olsen-Rasmussen M.S., Osborne J.,
RA Khristova M., Wohlhueter R.M.;
RL Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP REVIEW.
RX PubMed=12917449; DOI=10.1099/vir.0.18942-0;
RA Broyles S.S.;
RT "Vaccinia virus transcription.";
RL J. Gen. Virol. 84:2293-2303(2003).
CC -!- FUNCTION: Part of the DNA-dependent RNA polymerase which catalyzes the
CC transcription of viral DNA into RNA using the four ribonucleoside
CC triphosphates as substrates. Responsible for the transcription of
CC early, intermediate and late genes. DNA-dependent RNA polymerase
CC associates with the early transcription factor (ETF), itself composed
CC of D6 and A7, thereby allowing the early genes transcription. Late
CC transcription, and probably also intermediate transcription, require
CC newly synthesized RNA polymerase.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6;
CC -!- SUBUNIT: The DNA-dependent RNA polymerase used for intermediate and
CC late genes expression consists of eight subunits 147 kDa, 133 kDa, 35
CC kDa, 30 kDa, 22 kDa, 19 kDa, 18 kDa and 7 kDa totalling more than 500
CC kDa in mass. The same holoenzyme, with the addition of the
CC transcription-specificity factor RAP94, is used for early gene
CC expression.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Note=All the enzymes and
CC other proteins required to synthesize early mRNAs are packaged within
CC the virion core along with the DNA genome. This is necessary because
CC viral early mRNAs are synthesized within minutes after virus entry into
CC the cell and are extruded through pores in the core particle.
CC -!- SIMILARITY: Belongs to the poxviridae DNA-directed RNA polymerase 147
CC kDa subunit family. {ECO:0000305}.
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DR EMBL; U94848; AAB96506.1; -; Genomic_DNA.
DR EMBL; AY603355; AAT10488.1; -; Genomic_DNA.
DR PIR; T37366; T37366.
DR PDB; 6RIE; EM; 3.10 A; A=1-1286.
DR PDBsum; 6RIE; -.
DR SMR; O57204; -.
DR Proteomes; UP000159908; Genome.
DR Proteomes; UP000172909; Genome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 4.10.860.120; -; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR SMART; SM00663; RPOLA_N; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-directed RNA polymerase; Nucleotidyltransferase;
KW Transcription; Transferase; Virion.
FT CHAIN 1..1286
FT /note="DNA-directed RNA polymerase 147 kDa polypeptide"
FT /id="PRO_0000073918"
FT STRAND 3..11
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 14..17
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 30..32
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 34..37
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 50..52
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 56..58
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 64..66
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 72..74
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 76..78
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 79..85
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 86..89
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 91..94
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 100..102
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 108..110
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 114..117
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 121..124
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 132..134
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 142..144
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 146..153
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 154..156
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 162..169
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 174..176
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 177..181
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 186..189
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 190..193
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 195..197
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 206..210
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 213..217
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 219..229
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 232..234
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 237..240
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 241..248
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 250..252
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 262..265
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 272..276
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 278..292
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 294..296
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 300..303
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 305..308
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 312..316
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 318..321
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 322..329
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 330..332
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 334..339
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 340..343
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 344..347
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 350..352
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 365..369
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 375..379
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 386..388
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 389..396
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 398..404
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 406..408
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 409..413
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 416..418
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 420..424
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 429..437
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 441..444
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 448..450
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 459..470
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 472..474
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 476..483
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 484..486
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 500..508
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 515..517
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 525..527
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 530..532
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 541..548
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 551..571
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 579..581
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 583..585
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 586..614
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 623..655
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 660..667
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 673..680
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 697..699
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 700..702
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 713..716
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 722..724
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 728..763
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 766..768
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 774..776
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 779..784
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 785..787
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 792..794
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 796..799
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 810..820
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 824..826
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 827..830
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 835..840
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 842..844
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 851..853
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 857..874
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 875..878
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 883..892
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 895..897
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 902..918
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 926..943
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 961..969
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 976..985
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 986..995
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 1001..1003
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 1006..1012
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 1015..1023
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 1024..1029
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 1034..1046
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 1048..1060
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 1062..1076
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 1078..1092
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 1106..1115
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 1117..1129
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 1130..1135
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 1142..1144
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 1147..1153
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 1156..1169
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 1179..1189
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 1191..1194
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 1202..1204
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 1208..1214
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 1219..1226
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 1228..1231
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 1235..1240
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 1248..1251
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 1252..1256
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 1259..1262
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 1265..1281
FT /evidence="ECO:0007829|PDB:6RIE"
SQ SEQUENCE 1286 AA; 146886 MW; EE51E65E4BBCA2D7 CRC64;
MAVISKVTYS LYDQKEINAT DIIISHVKND DDIGTVKDGR LGAMDGALCK TCGKTELECF
GHWGKVSIYK THIVKPEFIS EIIRLLNYIC IHCGLLRSRE PYSDDINLKE LSGHALRRLK
DKILSKKKSC WNSECMQPYQ KITFSKKKVC FVNKLDDINV PNSLIYQKLI SIHEKFWPLL
EIHQYPANLF YTDYFPIPPL IIRPAISFWI DSIPKETNEL TYLLGMIVKN CNLNADEQVI
QKAVIEYDDI KIISNNTSSI NLSYITSGKN NMIRSYIVAR RKDQTARSVI GPSTSITVNE
VGMPAYIRNT LTEKIFVNAF TVDKVKQLLA SNQVKFYFNK RLNQLTRIRQ GKFIKNKIHL
LPGDWVEVAV QEYTSIIFGR QPSLHRYNVI ASSIRATEGD TIKISPGIVN SQNADFDGDE
EWMILEQNPK AVIEQSILMY PTTLLKHDIH GAPVYGSIQD EIVAAYSLFR IQDLCLDEVL
NILGKYGREF DPKGKCKFSG KDIYTYLIGE KINYPGLLKD GEIIANDVDS NFVVAMRHLS
LAGLLSDHKS NVEGINFIIK SSYVFKRYLS IYGFGVTFKD LRPNSTFTNK LEAINVEKIE
LIKEAYAKYL NDVRDGKIVP LSKALEADYV ESMLSNLTNL NIREIEEHMR QTLIDDPDNN
LLKMAKAGYK VNPTELMYIL GTYGQQRIDG EPAETRVLGR VLPYYLPDSK DPEGRGYILN
SLTKGLTGSQ YYFSMLVARS QSTDIVCETS RTGTLARKII KKMEDMVVDG YGQVVIGNTL
IKYAANYTKI LGSVCKPVDL IYPDESMTWY LEISALWNKI KQGFVYSQKQ KLAKKTLAPF
NFLVFVKPTT EDNAIKVKDL YDMIHNVIDD VREKYFFTVS NIDFMEYIFL THLNPSRIRI
TKETAITIFE KFYEKLNYTL GGGTPIGIIS AQVLSEKFTQ QALSSFHTTE KSGAVKQKLG
FNEFNNLTNL SKNKTEIITL VSDDISKLQS VKINFEFVCL GELNPNITLR KETDRYVVDI
IVNRLYIKRA EITELVVEYM IERFISFSVI VKEWGMETFI EDEDNIRFTV YLNFVEPEEL
NLSKFMMVLP GAANKGKISK FKIPISDYTG YDDFNQTKKL NKMTVELMNL KELGSFDLEN
VNVYPGVWNT YDIFGIEAAR EYLCEAMLNT YGEGFDYLYQ PCDLLASLLC ASYEPESVNK
FKFGAASTLK RATFGDNKAL LNAALHKKSE PINDNSSCHF FSKVPNIGTG YYKYFIDLGL
LMRMERKLSD KISSQKIKEM EETEDF
