ID   RP147_VACCW             Reviewed;        1287 AA.
AC   P07392; P07619; Q80HW6;
DT   01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1988, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=DNA-directed RNA polymerase 147 kDa polypeptide;
DE            EC=2.7.7.6;
GN   Name=RPO147; OrderedLocusNames=VACWR098; ORFNames=J6R;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3517852; DOI=10.1073/pnas.83.10.3141;
RA   Broyles S.S., Moss B.;
RT   "Homology between RNA polymerases of poxviruses, prokaryotes, and
RT   eukaryotes: nucleotide sequence and transcriptional analysis of vaccinia
RT   virus genes encoding 147-kDa and 22-kDa subunits.";
RL   Proc. Natl. Acad. Sci. U.S.A. 83:3141-3145(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-111.
RX   PubMed=2987815; DOI=10.1093/nar/13.3.985;
RA   Plucienniczak A., Schroeder E., Zettlmeissl G., Streeck R.E.;
RT   "Nucleotide sequence of a cluster of early and late genes in a conserved
RT   segment of the vaccinia virus genome.";
RL   Nucleic Acids Res. 13:985-998(1985).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   REVIEW.
RX   PubMed=12917449; DOI=10.1099/vir.0.18942-0;
RA   Broyles S.S.;
RT   "Vaccinia virus transcription.";
RL   J. Gen. Virol. 84:2293-2303(2003).
CC   -!- FUNCTION: Part of the DNA-dependent RNA polymerase which catalyzes the
CC       transcription of viral DNA into RNA using the four ribonucleoside
CC       triphosphates as substrates. Responsible for the transcription of
CC       early, intermediate and late genes. DNA-dependent RNA polymerase
CC       associates with the early transcription factor (ETF), itself composed
CC       of D6 and A7, thereby allowing the early genes transcription. Late
CC       transcription, and probably also intermediate transcription, require
CC       newly synthesized RNA polymerase.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: The DNA-dependent RNA polymerase used for intermediate and
CC       late genes expression consists of eight subunits 147 kDa, 133 kDa, 35
CC       kDa, 30 kDa, 22 kDa, 19 kDa, 18 kDa and 7 kDa totalling more than 500
CC       kDa in mass. The same holoenzyme, with the addition of the
CC       transcription-specificity factor RAP94, is used for early gene
CC       expression.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Note=All the enzymes and
CC       other proteins required to synthesize early mRNAs are packaged within
CC       the virion core along with the DNA genome. This is necessary because
CC       viral early mRNAs are synthesized within minutes after virus entry into
CC       the cell and are extruded through pores in the core particle.
CC   -!- SIMILARITY: Belongs to the poxviridae DNA-directed RNA polymerase 147
CC       kDa subunit family. {ECO:0000305}.
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DR   EMBL; M13209; AAB59835.1; -; Genomic_DNA.
DR   EMBL; X01978; CAA26020.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89377.1; -; Genomic_DNA.
DR   PIR; B25734; RNVZ47.
DR   SMR; P07392; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0039695; P:DNA-templated viral transcription; NAS:UniProtKB.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.132.30; -; 1.
DR   Gene3D; 4.10.860.120; -; 1.
DR   InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR   InterPro; IPR000722; RNA_pol_asu.
DR   InterPro; IPR006592; RNA_pol_N.
DR   InterPro; IPR007080; RNA_pol_Rpb1_1.
DR   InterPro; IPR007066; RNA_pol_Rpb1_3.
DR   InterPro; IPR007083; RNA_pol_Rpb1_4.
DR   InterPro; IPR007081; RNA_pol_Rpb1_5.
DR   InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR   InterPro; IPR038120; Rpb1_funnel_sf.
DR   PANTHER; PTHR19376; PTHR19376; 1.
DR   Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR   Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR   Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR   Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR   Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR   SMART; SM00663; RPOLA_N; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase; Virion.
FT   CHAIN           1..1287
FT                   /note="DNA-directed RNA polymerase 147 kDa polypeptide"
FT                   /id="PRO_0000073920"
FT   CONFLICT        62
FT                   /note="H -> Q (in Ref. 2; CAA26020)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        83
FT                   /note="I -> T (in Ref. 2; CAA26020)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1007..1014
FT                   /note="HSLFEKKQ -> ITLRKET (in Ref. 3; AAO89377)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1025
FT                   /note="K -> R (in Ref. 3; AAO89377)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1114
FT                   /note="C -> D (in Ref. 3; AAO89377)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1287 AA;  146949 MW;  570BB7DA1D4EEB76 CRC64;
     MAVISKVTYS LYDQKEINAT DIIISHVKND DDIGTVKDGR LGAMDGALCK TCGKTELECF
     GHWGKVSIYK THIVKPEFIS EIIRLLNHIC IHCGLLRSRE PYSDDINLKE LSGHALRRLK
     DKILSKKKSC WNSECMQPYQ KITFSKKKVC FVNKLDDINV PNSLIYQKLI SIHEKFWPLL
     EIHQYPANLF YTDYFPIPPL IIRPAISFWI DSIPKETNEL TYLLGMIVKN CNLNADEQVI
     QKAVIEYDDI KIISNNTSSI NLSYITSGKN NMIRSYIVAR RKDQTARSVI GPSTSITVNE
     VGMPAYIRNT LTEKIFVNAF TVDKVKQLLA SNQVKFYFNK RLNQLTRIRQ GKFIKNKIHL
     LPGDWVEVAV QEYTSIIFGR QPSLHRYNVI ASSIRATEGD TIKISPGIAN SQNADFDGDE
     EWMILEQNPK AVIEQSILMY PTTLLKHDIH GAPVYGSIQD EIVAAYSLFR IQDLCLDEVL
     NILGKYGREF DPKGKCKFSG KDIYTYLIGE KINYPGLLKD GEIIANDVDS NFVVAMRHLS
     LAGLLSDHKS NVEGINFIIK SSYVFKRYLS IYGFGVTFKD LRPNSTFTNK LEAINVEKIE
     LIKEAYAKYL NDVRDGKIVP LSKALEADYV ESMLSNLTNL NIREIEEHMR QTLIDDPDNN
     LLKMAKAGYK VNPTELMYIL GTYGQQRIDG EPAETRVLGR VLPYYLPDSK DPEGRGYILN
     SLTKGLTGSQ YYFSMLVARS QSTDIVCETS RTGTLARKII KKMEDMVVDG YGQVVIGNTL
     IKYAANYTKI LGSVCKPVDL IYPDESMTWY LEISALWNKI KQGFVYSQKQ KLAKKTLAPF
     NFLVFVKPTT EDNAIKVKDL YDMIHNVIDD VREKYFFTVS NIDFMEYIFL THLNPSRIRI
     TKETAITIFE KFYEKLNYTL GGGTPIGIIS AQVLSEKFTQ QALSSFHTTE KSGAVKQKLG
     FNEFNNLTNL SKNKTEIITL VSDDISKLQS VKINFEFVCL GELNPDHSLF EKKQDRYVVD
     IIVNKLYIKR AEITELVVEY MIERFISFSV IVKEWGMETF IEDEDNIRFT VYLNFVEPEE
     LNLSKFMMVL PGAANKGKIS KFKIPISDYT GYDCFNQTKK LNKMTVELMN LKELGSFDLE
     NVNVYPGVWN TYDIFGIEAA REYLCEAMLN TYGEGFDYLY QPCDLLASLL CASYEPESVN
     KFKFGAASTL KRATFGDNKA LLNAALHKKS EPINDNSSCH FFSKVPNIGT GYYKYFIDLG
     LLMRMERKLS DKISSQKIKE MEETEDF