ID   RP147_VARV              Reviewed;        1286 AA.
AC   P0DOO2; P33053;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2019, sequence version 1.
DT   03-AUG-2022, entry version 13.
DE   RecName: Full=DNA-directed RNA polymerase 147 kDa polypeptide;
DE            EC=2.7.7.6;
GN   Name=RPO147; ORFNames=J6R, L6R;
OS   Variola virus.
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=10255;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Bangladesh-1975;
RX   PubMed=8264798; DOI=10.1038/366748a0;
RA   Massung R.F., Esposito J.J., Liu L.I., Qi J., Utterback T.R., Knight J.C.,
RA   Aubin L., Yuran T.E., Parsons J.M., Loparev V.N., Selivanov N.A.,
RA   Cavallaro K.F., Kerlavage A.R., Mahy B.W.J., Venter J.C.;
RT   "Potential virulence determinants in terminal regions of variola smallpox
RT   virus genome.";
RL   Nature 366:748-751(1993).
CC   -!- FUNCTION: Part of the DNA-dependent RNA polymerase which catalyzes the
CC       transcription of viral DNA into RNA using the four ribonucleoside
CC       triphosphates as substrates. Responsible for the transcription of
CC       early, intermediate and late genes. DNA-dependent RNA polymerase
CC       associates with the early transcription factor (ETF) thereby allowing
CC       the early genes transcription. Late transcription, and probably also
CC       intermediate transcription, require newly synthesized RNA polymerase
CC       (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: The DNA-dependent RNA polymerase used for intermediate and
CC       late genes expression consists of eight subunits (147) kDa, (133) kDa,
CC       (35) kDa, (30) kDa, (22) kDa, (19) kDa, (18) kDa and (7) kDa totalling
CC       more than 500 kDa in mass. The same holoenzyme, with the addition of
CC       the transcription-specificity factor RAP94, is used for early gene
CC       expression (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Note=All the enzymes and
CC       other proteins required to synthesize early mRNAs are packaged within
CC       the virion core along with the DNA genome. This is necessary because
CC       viral early mRNAs are synthesized within minutes after virus entry into
CC       the cell and are extruded through pores in the core particle (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae DNA-directed RNA polymerase 147
CC       kDa subunit family. {ECO:0000305}.
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DR   EMBL; L22579; AAA60831.1; -; Genomic_DNA.
DR   PIR; T28521; T28521.
DR   RefSeq; NP_042127.1; NC_001611.1.
DR   SMR; P0DOO2; -.
DR   GeneID; 1486469; -.
DR   KEGG; vg:1486469; -.
DR   Proteomes; UP000119805; Genome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.132.30; -; 1.
DR   Gene3D; 4.10.860.120; -; 1.
DR   InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR   InterPro; IPR000722; RNA_pol_asu.
DR   InterPro; IPR006592; RNA_pol_N.
DR   InterPro; IPR007080; RNA_pol_Rpb1_1.
DR   InterPro; IPR007066; RNA_pol_Rpb1_3.
DR   InterPro; IPR007083; RNA_pol_Rpb1_4.
DR   InterPro; IPR007081; RNA_pol_Rpb1_5.
DR   InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR   InterPro; IPR038120; Rpb1_funnel_sf.
DR   PANTHER; PTHR19376; PTHR19376; 1.
DR   Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR   Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR   Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR   Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR   Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR   SMART; SM00663; RPOLA_N; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Early protein; Nucleotidyltransferase;
KW   Transcription; Transferase; Virion.
FT   CHAIN           1..1286
FT                   /note="DNA-directed RNA polymerase 147 kDa polypeptide"
FT                   /id="PRO_0000448123"
SQ   SEQUENCE   1286 AA;  146783 MW;  EE9965ACB1DFA93B CRC64;
     MAVISKVTYS LYDQKEINAT DIIISHVKND DDIGTVKDGR LGAMDGALCK TCGKTELECF
     GHWGKVSIYK THIVKPEFIS EIIRLLNHIC IHCGLLRSRE PYSNDINLKE LSGHALRRLK
     DKILSKKKSC WNSECMQPYQ KISFSKKKVC FVNKLDDINV PNSLIYQKLI SIHEKFWPLL
     EIYQYPANLF YTDYFPIPPL IIRPAISFWI DSIPKETNEL TYLLGMIVKN CNLNADEQVI
     QKAVIEYDDI KIISNNTTSI NLSYITSGKN NMIRSYIVAR RKDQTARSVI GPSTSITVNE
     VGMPAYIRNT LTEKIFVNAF TVNKVKQLLA SNQVKFYFNK RLNQLTRIRQ GKFIKNKIHL
     LPGDWVEVAV QEYTSIIFGR QPSLHRYNVI ASSIRATEGD TIKISPGIAN SQNADFDGDE
     EWMILEQNPK AVVEQSILMY PTTLLKHDIH GAPVYGSIQD EIVAAYSLFR IQDLCLDEVL
     NILGKYGREF DPKGKCKFSG KDIYTYLIGE KINYPGLLKD GEIIANDVDS NFVVAMRHLS
     LAGLLSDHKS NVEGINFIIK SSYVFKRYLS IYGFGVTFKD LRPNSTFTNK LEAINVEKIE
     LIKEAYAKYL KDVRDGKIVP LSKALEADYV ESMLSNLTNL NIREIEEHMR QTLIDNPDNN
     LLKMAKAGYK VNPTELMYIL GTYGQQRIDG EPAETRVLGR VLPYYLPDSK DPEGRGYILN
     SLTKGLTGSQ YYFSMLVARS QSTDIVCETS RTGTLARKII KKMEDMVVDG YGQVVIGNTL
     IKYAANYTKI LGSVCKPVDL IYPDESMTWY LEISALWNKI KQGFVYSQKQ KLAKKTLAPF
     NFLVFVKPTT EDNAIKVKDL YDMIHNVIDD VREKYFFTVS NIDFMEYIFL THLNPSRIRI
     TKETAITIFE KFYEKLNYTL GGGTPIGIIS AQVLSEKFTQ QALSSFHTTE KSGAVKQKLG
     FNEFNNLTNL SKNKTEIITL VSDDISKLQS VKINFEFVCL GELNPNITLR KETDRYVVDI
     IVNRLYIKRA EITELVVEYM IERFISFSVI VKEWGMETFI EDEDNIRFTV YLNFVEPEEL
     NLSKFMMVLP GAANKGKISK FKIPISDYTG YDDFNQTKKL NKMTVELMNL KELGSFDLEN
     VNVYPGVWNT YDIFGIEAAR GYLCEAMLNT YGEGFDYLYQ PCDLLASLLC ASYEPESVNK
     FKFGAASTLK RATFGDNKAL LNAALHKKSE PINDNSSCHF FSKVPNIGTG YYKYFIDLGL
     LMRMERKLSD KISSQKIKEM EETEDF