ID   RP18_VACCA              Reviewed;         161 AA.
AC   Q76ZS0;
DT   21-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 55.
DE   RecName: Full=DNA-directed RNA polymerase 18 kDa subunit;
DE            EC=2.7.7.6;
GN   Name=RPO18; OrderedLocusNames=MVA104R, ACAM3000_MVA_104; ORFNames=D7R;
OS   Vaccinia virus (strain Ankara) (VACV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=126794;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=9601507; DOI=10.1006/viro.1998.9123;
RA   Antoine G., Scheiflinger F., Dorner F., Falkner F.G.;
RT   "The complete genomic sequence of the modified vaccinia Ankara strain:
RT   comparison with other orthopoxviruses.";
RL   Virology 244:365-396(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Isolate Acambis 3000;
RA   Esposito J.J., Frace M., Sammons S.A., Olsen-Rasmussen M.S., Osborne J.,
RA   Khristova M., Wohlhueter R.M.;
RL   Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   REVIEW.
RX   PubMed=12917449; DOI=10.1099/vir.0.18942-0;
RA   Broyles S.S.;
RT   "Vaccinia virus transcription.";
RL   J. Gen. Virol. 84:2293-2303(2003).
CC   -!- FUNCTION: Part of the DNA-dependent RNA polymerase which catalyzes the
CC       transcription of viral DNA into RNA using the four ribonucleoside
CC       triphosphates as substrates. Responsible for the transcription of
CC       early, intermediate and late genes. DNA-dependent RNA polymerase
CC       associates with the early transcription factor (ETF), itself composed
CC       of D6 and A7, thereby allowing the early genes transcription. Late
CC       transcription, and probably also intermediate transcription, require
CC       newly synthesized RNA polymerase.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: The DNA-dependent RNA polymerase used for intermediate and
CC       late genes expression consists of eight subunits 147 kDa, 133 kDa, 35
CC       kDa, 30 kDa, 22 kDa, 19 kDa, 18 kDa and 7 kDa totalling more than 500
CC       kDa in mass. The same holoenzyme, with the addition of the
CC       transcription-specificity factor RAP94, is used for early gene
CC       expression (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Note=All the enzymes and
CC       other proteins required to synthesize early mRNAs are packaged within
CC       the virion core along with the DNA genome. This is necessary because
CC       viral early mRNAs are synthesized within minutes after virus entry into
CC       the cell and are extruded through pores in the core particle.
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC   -!- SIMILARITY: Belongs to the poxviridae DNA-directed RNA polymerase 18
CC       kDa subunit family. {ECO:0000305}.
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DR   EMBL; U94848; AAB96516.1; -; Genomic_DNA.
DR   EMBL; AY603355; AAT10502.1; -; Genomic_DNA.
DR   RefSeq; YP_232994.1; NC_006998.1.
DR   PDB; 6RIE; EM; 3.10 A; G=1-161.
DR   PDBsum; 6RIE; -.
DR   SMR; Q76ZS0; -.
DR   DNASU; 3707568; -.
DR   GeneID; 3707568; -.
DR   KEGG; vg:3707568; -.
DR   Proteomes; UP000159908; Genome.
DR   Proteomes; UP000172909; Genome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019083; P:viral transcription; IEA:InterPro.
DR   InterPro; IPR004973; DNA-dir_RNA_pol_18kDa_poxviral.
DR   Pfam; PF03293; Pox_RNA_pol; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-directed RNA polymerase; Early protein;
KW   Nucleotidyltransferase; Transcription; Transferase; Virion.
FT   CHAIN           1..161
FT                   /note="DNA-directed RNA polymerase 18 kDa subunit"
FT                   /id="PRO_0000099150"
FT   STRAND          3..14
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   HELIX           16..18
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   HELIX           23..35
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   STRAND          43..50
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   STRAND          52..54
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   STRAND          59..61
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   STRAND          63..77
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   STRAND          84..90
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   STRAND          94..96
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   STRAND          98..101
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   STRAND          104..107
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   STRAND          137..144
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   STRAND          151..158
FT                   /evidence="ECO:0007829|PDB:6RIE"
SQ   SEQUENCE   161 AA;  17911 MW;  BA27905B8E507954 CRC64;
     MSSFVTNGYL PVTLEPHELT LDIKTNIRNA VYKTYLHREI SGKMAKKIEI REDVELPLGE
     IVNNSVVINV PCVITYAYYH VGDIVRGTLN IEDESNVTIQ CGDLICKLSR DSGTVSFSDS
     KYCFFRNGNA YDNGSEVTAV LMEAQQGIES SFVFLANIVD S