ID   RP19_FOWPN              Reviewed;         167 AA.
AC   Q05569; O93019;
DT   29-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=DNA-directed RNA polymerase 19 kDa subunit;
DE            EC=2.7.7.6;
GN   Name=RPO19; OrderedLocusNames=FPV169; ORFNames=FP-A5, FPA5;
OS   Fowlpox virus (strain NVSL) (FPV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Avipoxvirus.
OX   NCBI_TaxID=928301;
OH   NCBI_TaxID=7742; Vertebrata.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2157318; DOI=10.1016/0168-1702(90)90007-x;
RA   Kumar S., Boyle D.B.;
RT   "Mapping of a major early/late gene of fowlpox virus.";
RL   Virus Res. 15:175-186(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=10729156; DOI=10.1128/jvi.74.8.3815-3831.2000;
RA   Afonso C.L., Tulman E.R., Lu Z., Zsak L., Kutish G.F., Rock D.L.;
RT   "The genome of fowlpox virus.";
RL   J. Virol. 74:3815-3831(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-97.
RC   STRAIN=FP-9 / Isolate HP-440;
RX   PubMed=9420213; DOI=10.1128/jvi.72.1.170-179.1998;
RA   Boulanger D., Green P., Smith T., Czerny C.P., Skinner M.A.;
RT   "The 131-amino-acid repeat region of the essential 39-kilodalton core
RT   protein of fowlpox virus FP9, equivalent to vaccinia virus A4L protein, is
RT   nonessential and highly immunogenic.";
RL   J. Virol. 72:170-179(1998).
CC   -!- FUNCTION: Part of the DNA-dependent RNA polymerase which catalyzes the
CC       transcription of viral DNA into RNA using the four ribonucleoside
CC       triphosphates as substrates. Responsible for the transcription of
CC       early, intermediate and late genes. DNA-dependent RNA polymerase
CC       associates with the early transcription factor (ETF) thereby allowing
CC       the early genes transcription. Late transcription, and probably also
CC       intermediate transcription, require newly synthesized RNA polymerase
CC       (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: The DNA-dependent RNA polymerase used for intermediate and
CC       late genes expression consists of eight subunits (147) kDa, 133 kDa, 35
CC       kDa, 30 kDa, 22 kDa, 19 kDa, 18 kDa and 7 kDa totalling more than 500
CC       kDa in mass. The same holoenzyme, with the addition of the
CC       transcription-specificity factor RAP94, is used for early gene
CC       expression (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Note=All the enzymes and
CC       other proteins required to synthesize early mRNAs are packaged within
CC       the virion core along with the DNA genome. This is necessary because
CC       viral early mRNAs are synthesized within minutes after virus entry into
CC       the cell and are extruded through pores in the core particle (By
CC       similarity). {ECO:0000250}.
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC       Expression seems to continue throughout virus infection.
CC   -!- SIMILARITY: Belongs to the poxviridae DNA-directed RNA polymerase 19
CC       kDa subunit family. {ECO:0000305}.
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DR   EMBL; X52461; CAA36694.1; -; Genomic_DNA.
DR   EMBL; AF198100; AAF44513.1; -; Genomic_DNA.
DR   EMBL; AJ005164; CAA06407.1; -; Genomic_DNA.
DR   PIR; A60013; A60013.
DR   RefSeq; NP_039132.1; NC_002188.1.
DR   SMR; Q05569; -.
DR   GeneID; 1486717; -.
DR   KEGG; vg:1486717; -.
DR   Proteomes; UP000008597; Genome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   InterPro; IPR007984; DNA-dir_RNA_Pol_19kDa_poxvir.
DR   Pfam; PF05320; Pox_RNA_Pol_19; 1.
DR   PIRSF; PIRSF000743; RPO19; 1.
PE   2: Evidence at transcript level;
KW   DNA-directed RNA polymerase; Early protein; Nucleotidyltransferase;
KW   Reference proteome; Transcription; Transferase; Virion.
FT   CHAIN           1..167
FT                   /note="DNA-directed RNA polymerase 19 kDa subunit"
FT                   /id="PRO_0000099149"
FT   REGION          14..41
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        18..32
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   167 AA;  19500 MW;  D67E10A724179C37 CRC64;
     MDNSMDINDI LLSDDNDYKS YDEDDDSISD IGETSDDCCT TKQSDSRIES FKFDETTQSP
     HPKQLSERIK AIKQRYTRRI SLFEITGILS ESYNLLQRGR IPLLNDLTEE TFKDSIINIM
     FKEIEQGNCP IVIQKNGELL SLTDFDKKGV QYHLDYIKTI WRNQRKL