RP19_VAR67
ID RP19_VAR67 Reviewed; 164 AA.
AC P33813;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=DNA-directed RNA polymerase 19 kDa subunit;
DE EC=2.7.7.6;
GN Name=RPO19; ORFNames=A5R;
OS Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX NCBI_TaxID=587200;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1666548;
RA Shchelkunov S.N., Marennikova S.S., Totmenin A.V., Blinov V.M.,
RA Chizhikov V.E., Gutorov V.V., Safronov P.F., Pozdnyakov S.G.,
RA Shelukhina E.M., Gashnikov P.V., Anjaparidze O.G., Sandakhchiev L.S.;
RT "Creation of a clone library of fragments from the natural variola virus
RT and study of the structural and functional organization of viral genes from
RT a circle of hosts.";
RL Dokl. Akad. Nauk SSSR 321:402-406(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=8384129; DOI=10.1016/0014-5793(93)80041-r;
RA Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.;
RT "Genes of variola and vaccinia viruses necessary to overcome the host
RT protective mechanisms.";
RL FEBS Lett. 319:80-83(1993).
CC -!- FUNCTION: Part of the DNA-dependent RNA polymerase which catalyzes the
CC transcription of viral DNA into RNA using the four ribonucleoside
CC triphosphates as substrates. Responsible for the transcription of
CC early, intermediate and late genes. DNA-dependent RNA polymerase
CC associates with the early transcription factor (ETF) thereby allowing
CC the early genes transcription. Late transcription, and probably also
CC intermediate transcription, require newly synthesized RNA polymerase
CC (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6;
CC -!- SUBUNIT: The DNA-dependent RNA polymerase used for intermediate and
CC late genes expression consists of eight subunits (147) kDa, 133 kDa, 35
CC kDa, 30 kDa, 22 kDa, 19 kDa, 18 kDa and 7 kDa totalling more than 500
CC kDa in mass. The same holoenzyme, with the addition of the
CC transcription-specificity factor RAP94, is used for early gene
CC expression (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Note=All the enzymes and
CC other proteins required to synthesize early mRNAs are packaged within
CC the virion core along with the DNA genome. This is necessary because
CC viral early mRNAs are synthesized within minutes after virus entry into
CC the cell and are extruded through pores in the core particle (By
CC similarity). {ECO:0000250}.
CC -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC Expression seems to continue throughout virus infection.
CC -!- SIMILARITY: Belongs to the poxviridae DNA-directed RNA polymerase 19
CC kDa subunit family. {ECO:0000305}.
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DR EMBL; X69198; CAA49050.1; -; Genomic_DNA.
DR EMBL; X67116; CAA47518.1; -; Genomic_DNA.
DR PIR; F36848; F36848.
DR RefSeq; NP_042153.1; NC_001611.1.
DR SMR; P33813; -.
DR GeneID; 1486535; -.
DR KEGG; vg:1486535; -.
DR Proteomes; UP000002060; Genome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 3.90.940.10; -; 1.
DR InterPro; IPR007984; DNA-dir_RNA_Pol_19kDa_poxvir.
DR InterPro; IPR036161; RPB6/omega-like_sf.
DR Pfam; PF05320; Pox_RNA_Pol_19; 1.
DR PIRSF; PIRSF000743; RPO19; 1.
PE 2: Evidence at transcript level;
KW DNA-directed RNA polymerase; Early protein; Nucleotidyltransferase;
KW Reference proteome; Transcription; Transferase; Virion.
FT CHAIN 1..164
FT /note="DNA-directed RNA polymerase 19 kDa subunit"
FT /id="PRO_0000099148"
FT REGION 1..39
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..32
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 164 AA; 18981 MW; BC7CF3E24740C327 CRC64;
MADTDDIIDY ESDDLTEYED DDEEEEDGES LETSDIDPKS SYKIVESAST HIEDAHSNLK
HIGNHISALK RRYTRRISLF EIAGIIAESY NLLQRGRLPL VSEFSNETMK QNMLHVIIQE
IEEGSCPIVI EKNGELLSVN DFDKDGLKFH LDYIIKIWKL QKRY